解剖学报 ›› 2021, Vol. 52 ›› Issue (5): 720-727.doi: 10.16098/j.issn.0529-1356.2021.05.008

• 细胞和分子生物学 • 上一篇    下一篇

鼻内针刺对兔变态反应性鼻炎模型鼻黏膜病理学的作用及瞬时感受器电位香草酸受体1-P物质轴相关的调控机制#br#

刘莉莉1 巩政1 矫璐璐1 刘思溟1 边芳子2 张琳婧1 刘巧平1 闫占峰1*
  

  1. 1.北京中医药大学东直门医院耳鼻喉科,北京 100700;  2.西安市中医医院耳鼻喉科,西安 710021
  • 收稿日期:2020-12-15 修回日期:2021-02-22 出版日期:2021-10-06 发布日期:2021-10-06
  • 通讯作者: 闫占峰 E-mail:15210682430@163.com
  • 基金资助:
    中枢参与下的鼻内针刺治疗过敏性鼻炎神经免疫调控机制研究;CCR3/TRPV1/NP介导的鼻内针刺对过敏性鼻炎模型兔神经源性炎症调控机制;鼻内针法通过TRPV1途径对变应性鼻炎中CD4+T细胞增殖分化的调控机制研究

Effect of intranasal acupuncture on the pathology of nasal mucosa in rabbits with allergic rhinitis and related regulatory mechanism of transient receptor potential vanillic acid receptor 1-substance P axis#br#

LIU Li-li1 GONG Zheng1 JIAO Lu-lu1 LIU Si-ming1 BIAN Fang-zi2 ZHANG Lin-jing1 LIU Qiao-ping1 YAN Zhan-feng1*#br#   

  1. 1.Department of Otolaryngology, Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100700,China; 2.Department of Otolaryngology, Xi’an Hospital of Traditional Chinese Medicine, Xi’an 710021,China
  • Received:2020-12-15 Revised:2021-02-22 Online:2021-10-06 Published:2021-10-06
  • Contact: YAN Zhan-feng* E-mail:15210682430@163.com

摘要:

目的  通过鼻内针刺明确鼻黏膜的病理学变化并通过瞬时感受器电位香草酸受体1(TRPV1)-P物质(SP)轴在实验性变应性鼻炎鼻黏膜中分布及表达情况,探讨其可能的信号传导机制。 方法  采用卵清蛋白及氢氧化铝凝胶方法建立变应性鼻炎动物模型。25只新西兰大耳兔随机分成正常组、模型组、假针刺组、针刺外迎香组和鼻内针刺组,每组各5只。观察鼻内针刺对动物行为学、鼻黏膜病理学及动脉血中嗜酸性粒细胞(EOS)计数和IgE含量影响,采用HE染色法明确鼻黏膜EOS的分布,采用免疫组织化学方法观察鼻黏膜及TRPV1及SP的分布和表达情况。
  结果  行为学评分结果发现,与模型组相比,假针刺组行为学计分下降不明显,针刺外迎香组和鼻内针刺组行为学计分有下降趋势,鼻内针刺组下降程度更显著。血常规中EOS计数结果显示,模型组与正常组相比,EOS计数稍增多但差别无显著性(P>0.05);鼻内针刺组与模型组相比,EOS计数有减少趋势,差别也无显著性(P>0.05)。ELISA法检测IgE发现,与正常组相比,模型组血清中IgE含量明显增多(P<0.05);与模型组相比,假针刺组血清中IgE含量未见明显变化(P>0.05),针刺外迎香组和鼻内针刺组血清IgE含量有下降趋势(P<0.05),但鼻内针刺组血清IgE含量较针刺外迎香组下降更为明显,两组差异无统计学意义(P>0.05)。HE染色结果显示,与正常组相比,模型组和假针刺组EOS浸润明显增多, 差异有统计学意义(P<0.05);与模型组相比,鼻内针刺组和外迎香组EOS浸润程度减轻,差异有统计学意义(P<0.05)。免疫组织化学结果显示,与正常组相比,模型组和假针刺组鼻黏膜TRPV1及SP表达增多,差异有统计学意义(P<0.05);与模型组相比,鼻内针刺组和外迎香组TRPV1、SP表达明显减弱,差异有统计学意义(P<0.05)。   结论  鼻内针刺可以通过减少嗜酸性粒细胞在鼻黏膜的趋化及血清中IgE含量来缓解变应性鼻炎模型的鼻部症状。

关键词: 鼻内针刺, 瞬时感受器电位香草酸受体1, P物质, 变应性鼻炎, 免疫组织化学, 兔

Abstract:

Objective  To investigate the pathological changes of nasal mucosa by intranasal acupuncture,and to explore the possible signal transduction mechanism〓through the distribution and expression of transient receptor potential vanillic acid receptor 1 (TRPV1)-substance P (SP) axis in nasal mucosa of experimental allergic rhinitis.     Methods  An animal model of allergic rhinitis was constructed by ovalbumin and aluminium hydroxide gel treatment. Twenty five New Zealand rabbits were randomly divided into normal group, model group, sham acupuncture group, acupuncture Wai Ying Xiang group and intranasal acupuncture group, with 5 rabbits in each group. To observe the effect of intranasal acupuncture on animal behavior, nasal mucosa pathology, eosinophils (EOS) count and IgE content in arterial blood. In addition, HE staining was used to determine the distribution of EOS in nasal mucosa and immunohistochemistry was used to observe the distribution and expression of TRPV1 and SP in nasal mucosa membrane.     Results  Compared with the model group, the behavioral score of the sham acupuncture group did not decrease significantly, while the behavioral score of the Wai Ying Xiang acupuncture group and the intranasal acupuncture group showed a downward trend, and the decrease degree of the intranasal acupuncture group was more significant. Compared with the normal group, the eosinophil count in the model group increased slightly(P>0.05); Compared with the model group, the number of eosinophils in the intranasal acupuncture group decreased (P>0.05). Compared with the normal group, the serum IgE content in the model group increased  significantly (P<0.05). Compared with the model group, there was no significant change in the sham acupuncture group (P>0.05). Compared with the model group, serum IgE content in the Wai Ying Xiang acupuncture group and the internal acupuncture group decreased significantly (P<0.05), but there was no statistical difference between the internal acupuncture group and the Wai Ying Xiang acpuncture group (P>0.05). HE staining found that eosinophilic distribution increased significantly in the model group and the sham acupuncture group compared with the normal group, and the difference was statistically significant (P<0.05). Compared with the model group, the distribution of eosinophil reduced in the nasal acupuncture group and the Wai Ying Xiang group. The difference was statistically significant (P<0.05). Immunohistochemical found that TRPV1 and SP expressed significantly in the nasal mucosa of the model group and the sham acupuncture group compared with the normal group, the difference was statistically significant (P<0.05). Compared with the model group, TRPV1 and SP expression decreased significantly in the intranasal acupuncture group and the external yingxiang group, the difference was statistically significant (P<0.05).     Conclusion  Intranasal acupuncture can alleviate the nasal symptoms of allergic rhinitis model by reducing the chemotaxis of eosinophils in nasal mucosa and the content of IgE in serum. 

Key words: Intranasal acupuncture, Transient receptor potential vanillic acid receptor 1, Substance P, Allergic rhinitis, Immunohistochemistry, Rabbit

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