丝裂原活化蛋白激酶激酶1在胃癌中的表达及其临床意义

doi:10.16098/j.issn.0529-1356.2022.03.006

解剖学报 ›› 2022, Vol. 53 ›› Issue (3): 309-316.doi: 10.16098/j.issn.0529-1356.2022.03.006

丝裂原活化蛋白激酶激酶1在胃癌中的表达及其临床意义

赵媛¹陶月佳¹ 余广福² 郭嘉欣¹ 李梦琪¹ 徐远义^1* 黄允宁^2*

1.宁夏医科大学基础医学院病理学系，银川750000 ; 2.宁夏回族自治区人民医院胃肠外科，银川 750000

收稿日期:2021-04-22 修回日期:2021-08-20 出版日期:2022-06-06 发布日期:2019-06-06
通讯作者: 徐远义;黄允宁 E-mail:nxxyy@hotmail.com
基金资助:
宁夏回族自治区重点研发项目;宁夏回族自治区重点研发项目;宁夏自然科学基金项目

Expression of mitogen-activated protein kinase kinase 1 in gastric cancer and its clinical significance

ZHAO Yuan¹ TAO Yue-jia¹ YU Guang-fu² GUO Jia-xin¹ LI Meng-qi¹ XU Yuan-yi^1* HUANG Yun-ning^2*#br#

1.Department of Pathology, Basic Medical College, Ningxia Medical University, Yinchuan 750000, China; 2.Department of Gastrointestinal Surgery, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan 750000, China

Received:2021-04-22 Revised:2021-08-20 Online:2022-06-06 Published:2019-06-06
Contact: XU Yuan-yi;HUANG Yun-ning E-mail:nxxyy@hotmail.com
Supported by:
the Key research and development projects of Ningxia Hui Autonomous Region; the Key research and development projects of Ningxia Hui Autonomous Region;Ningxia Natural Science Foundation Project

摘要/Abstract

摘要：

目的探讨丝裂原活化蛋白激酶激酶1（MAP2K1）在胃癌中的表达及其临床意义。方法免疫组织化学及Western blotting检测MAP2K1在胃癌组织和细胞中蛋白水平的表达；免疫荧光染色观察细胞形态及MAP2K1的表达位置；StarBase及Oncomine 数据库对MAP2K1进行数据挖掘，分析MAP2K1 mRNA在胃癌组织中的表达情况；UALCAN数据库分析MAP2K1 mRNA的表达与临床病理特征的相关性；Kaplan Meier-Plotter 在线分析工具进行生存分析; GEPIA2数据库挖掘MAP2K1与胃癌干细胞相关因子和耐药相关因子的关系。结果免疫组织化学、免疫荧光和Western blotting结果表明，在胃癌组织和细胞中MAP2K1蛋白为高表达，且MAP2K1表达在胃癌细胞质中。由多种数据库分析得出，MAP2K1 mRNA在胃癌组织的表达量高于正常胃组织，其表达量与胃癌分期、分级、淋巴结转移和患者性别密切相关，MAP2K1高表达组的胃癌患者总体生存率明显低于低表达组,其原因可能与胃癌干细胞特性和耐药性相关。结论 MAP2K1在胃癌中高表达，其表达水平可能通过调控干细胞相关因子与耐药相关因子影响患者不良预后。MAP2K1或可成为判断胃癌患者预后的新型诊断标志物。

关键词: 丝裂原活化蛋白激酶激酶1, 胃癌, 胃癌细胞系AGS和HGC-27, 免疫印迹法, 人

Abstract:

Objective To investigate the expression of mitogen-activated protein kinase kinase 1 (MAP2K1) in gastric cancer and its clinical significance. Methods Immunohistochemistry and Western blotting were used to detect the protein expression of MAP2K1 in gastric cancertissues and cells. The morphology and the expression position of MAP2K1 were observed by immunofluorescence. MAP2K1 mRNA expression in gastric cancer tissues was analyzed by data mining of Starbase database and Oncomine database. The correlation between MAP2K1 mRNA expression and clinicopathological features was analyzed by UALCAN database. Survival analysis was performed using Kaplan Meier-Plotter online analysis tools. GEPIA2 database mining the relationship between MAP2K1 and gastric cancer stem cell related factors and drug resistance related factors. Results Immunohistochemistry, immunofluorescence and Western blotting showed that MAP2K1 protein was highly expressed in gastric cancer tissues and cells, and MAP2K1 was expressed in the cytoplasm of gastric cancer. According to the analysis of various databases, the expression of MAP2K1 mRNA in gastric cancer tissue was higher than that in normal gastric tissue, and the expression of MAP2K1 mRNA was closely related to gastric cancer stage, grade, lymph node metastasis and patient gender, and the overall survival rate of gastric cancer patients in the group with high MAP2K1 mRNA expression was significantly lower than that in the group with low MAP2K1 mRNA expression, which may be related to the characteristics of gastric cancer stem cells and drug resistance. Conclusion MAP2K1 is highly expressed in gastric cancer, and its expression level may affect the poor prognosis of patients by regulating stem cell related factors and drug resistance related factors. MAP2K1 may be a new diagnostic marker to determine the prognosis of gastric cancer patients.

Key words: Mitogen-activated protein kinase kinase 1, Gastric cancer, Gastric cancer cell line AGS and HGC-27, Western blotting, Human

中图分类号:

R735.2

赵媛陶月佳余广福郭嘉欣李梦琪徐远义黄允宁. 丝裂原活化蛋白激酶激酶1在胃癌中的表达及其临床意义[J]. 解剖学报, 2022, 53(3): 309-316.

ZHAO Yuan TAO Yue-jia YU Guang-fu GUO Jia-xin LI Meng-qi XU Yuan-yi HUANG Yun-ning. Expression of mitogen-activated protein kinase kinase 1 in gastric cancer and its clinical significance[J]. Acta Anatomica Sinica, 2022, 53(3): 309-316.

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丝裂原活化蛋白激酶激酶1在胃癌中的表达及其临床意义

Expression of mitogen-activated protein kinase kinase 1 in gastric cancer and its clinical significance

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