石菖蒲挥发油对炎性痛大鼠脊髓背角中胶质纤维酸性蛋白、c-Jun氨基末端蛋白激酶和肿瘤坏死因子α表达的影响

doi:10.16098/j.issn.0529-1356.2023.01.003

摘要/Abstract

摘要：

目的探讨石菖蒲挥发油（VOA）对炎性痛大鼠脊髓背角中胶质纤维酸性蛋白（GFAP）、c-Jun氨基末端蛋白激酶（JNK）和肿瘤坏死因子α（TNF-α）表达的影响。方法 36只雄性SD大鼠随机分为：对照组（control）、假手术组（sham）、完全弗氏佐剂组（CFA）、5 g/（kg·d）低剂量VOA + CFA组（VOA-L+CFA）、10 g/（kg·d）中剂量VOA + CFA组（VOA-M+CFA)和20 g/（kg·d）高剂量VOA + CFA组（VOA-H+CFA)，共6组，每组6只，于连续灌胃给药的第22天取材。利用免疫荧光染色和Western blotting技术检测各组大鼠脊髓背角中GFAP、JNK和TNF-α的表达情况。结果免疫荧光染色及Western blotting结果表明，与control和sham组相比，CFA组中大鼠脊髓背角中GFAP、JNK和TNF-α阳性表达均显著增多（P<0.01）；与CFA组相比，不同剂量VOA处理组大鼠脊髓背角中GFAP、JNK和TNF-α阳性表达均减少，且VOA-H+CFA组大鼠脊髓背角中GFAP、JNK和TNF-α阳性表达减少更明显（P<0.05，P<0.01）。结论 VOA可降低CFA诱导的炎性痛大鼠脊髓背角中GFAP、JNK和TNF-α的表达。

关键词: 石菖蒲挥发油, 炎性痛, 胶质纤维酸性蛋白, c-Jun氨基末端蛋白激酶, 肿瘤坏死因子α, 免疫荧光, 免疫印迹法, 大鼠

Abstract:

Objective To investigate the influence of volatile oil from Acori graminei Rhizoma（VOA）on expressions of glial fibrillary acidic protein (GFAP), c-Jun N-terminal protein kainse (JNK) and tumour necrosis factor-α (TNF-α) in the spinal cord dorsal horn of imflammatory pain rats. Methods Totally 36 male SD rats were randomly divided into control group (control), sham-operated group (sham), complete Freund’s adjuvant group (CFA), 5 g/(kg·d) low dose VOA+CFA group (VOA-L+CFA), 10 g/(kg·d) medium dose VOA + CFA group (VOA-M+CFA) and 20 g/(kg·d) high dose VOA + CFA group (VOA-H+CFA). All animals were sacrificed immediately after continuous gavage administration for 22 days. The expressions of GFAP, JNK and TNF-α in the spinal cord dorsal horn of rats in each group were detected by immunofluorescence and Western blotting methods. Results The present results showed that the positive expressions of GFAP, JNK and TNF-α in the spinal cord dorsal horn of rats increased significantly in the CFA group, when compared to the control and sham groups (P< 0.01). The expressions of GFAP, JNK and TNF-α in the spinal cord dorsal horn of rats with VOA treatment reduced in the dose-dependent manner, when compared to the CFA group, the positive expressions of GFAP, JNK and TNF-α reduced significantly in the dorsal horn of the spinal cord of the VOA-H+CFA group (P<0.05, P<0.01). Conclusion VOA reduces the expressions of GFAP, JNK and TNF-α in the spinal cord dorsal horn of rats of CFA-induced inflammatory pain.

Key words:

Volatile oil from Acori graminei Rhizoma, Inflammatory pain, Glial fibrillary acidic protein, c-Jun N-terminal kinase, Tumour necrosis factor-α, , Immunofluorescence, Western blotting, Rat

中图分类号:

R338.2⁺2

张润恒杨翠珠李诗琪王姝涵王欣刘靖马宇昕. 石菖蒲挥发油对炎性痛大鼠脊髓背角中胶质纤维酸性蛋白、c-Jun氨基末端蛋白激酶和肿瘤坏死因子α表达的影响[J]. 解剖学报, 2023, 54(1): 23-29.

ZHANG Run-heng YANG Cui-zhu LI Shi-qi WANG Shu-han WANG Xin LIU Jing MA Yu-xin.

Effects of volatile oil from Acori graminei Rhizoma on glial fibrillary acidic protein, c-Jun N-terminal protein kinase and tumor necrosis factor-α expressions in the spinal cord dorsal horn of the inflammatory pain rats

[J]. Acta Anatomica Sinica, 2023, 54(1): 23-29.

参考文献

［1］Raja SN, Carr DB, Cohen M, et al. The revised International association for the study of pain definition of pain: concepts, challenges, and compromises ［J］. Pain，2020, 161(9): 1976-1982.

［2］Vos T, Abajobir AA, Abate KH, et al. Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990-2016: a systematic analysis for the global burden of disease study 2016 ［J］. Lancet，2017, 390(10100): 1211-1259.

［3］Cramer JD, Barnett ML, Anne S, et al. Nonopioid, Multimodal analgesia as first-line therapy after otolaryngology operations: primer on nonsteroidal anti-inflammatory drugs (NSAIDs) ［J］. Otolaryngol Head Neck Surg,2021, 164(4): 712-719.

［4］Herzberg DL, Sukumaran HP, Viscusi E. NSAIDs for analgesia in the era of COVID-19 ［J］. Reg Anesth Pain Med, 2020, 45(9): 677-678.

［5］Ye F, Xu Y, Lin F, et al. TNF-α suppresses SHOX2 expression via NF-κB signaling pathway and promotes intervertebral disc degeneration and related pain in a rat model ［J］. J Orthop Res, 2021, 39(8): 1745-1754.

［6］Liu X, He J, Gao J, et al. Fluorocitrate and neurotropin confer analgesic effects on neuropathic pain in diabetic rats via inhibition of astrocyte activation in the periaqueductal gray ［J］. Neurosci Lett, 2022, 768: 136378.

［7］Oh SH, Kim SW, Kim DJ, et al. Sec-O-glucosylhamaudol mitigates inflammatory processes and autophagy via p38/JNK MAPK signaling in a rat neuropathic pain model ［J］. Korean J Pain, 2021, 34(4): 405-416.

［8］Hu Y, Fang X, Wang J, et al. Astragalin attenuates AlCl3/D-galactose-induced aging-like disorders by inhibiting oxidative stress and neuroinflammation［J］. Neurotoxicology, 2022, 91:60-68.

［9］Wang N, Wang H, Li L, et al. β-asarone inhibits amyloid-β by promoting autophagy in a cell model of Alzheimer’s disease ［J］. Front Pharmacol, 2020, 10:1529.

［10］Bushnell MC, Ceko M, Low LA. Cognitive and emotional control of pain and its disruption in chronic pain［J］. Nat Rev Neurosci, 2013, 14(7): 502-511.

［11］Wu L, Zhuo M. Targeting the NMDA receptor subunit NR2B for the treatment of neuropathic pain ［J］. Neurotherapeutics, 2009, 6(4): 693-702.

［12］Gao Y, Ji R. Targeting astrocyte signaling for chronic pain ［J］. Neurotherapeutics, 2010, 7(4): 482-493.

［13］Li ShQ, Yang CZh, Liu HQ, et al. Effect of volatile oil of acorus calamus on glial fibrillary acidic protein and immediate early gene expression in the basolateral amygdala of rats with inflammatory pain［J］. Acta Anatomica Sinica, 2021, 52(2): 189-195.(in Chinese)

李诗琪，杨翠珠，刘鸿庆，等. 石菖蒲挥发油对炎症痛大鼠基底外侧杏仁核中胶质纤维酸性蛋白和即刻早期基因表达的影响［J］. 解剖学报. 2021, 52(2): 189-195.

［14］Tian J, Song T, Wang H, et al. Thalidomide alleviates bone cancer pain by down-regulating expressions of NF-kappaB and GFAP in spinal astrocytes in a mouse model ［J］. Int J Neurosci, 2019, 129(9): 896-903.

［15］Cai J, Yan Y, Zhang D, et al. Silencing oflncRNA Gm14461 alleviates pain in trigeminal neuralgia through inhibiting astrocyte activation ［J］. IUBMB Life, 2020, 72(12): 2663-2671.

［16］Kwiatkowski K, Piotrowska A, Rojewska E, et al. The RS504393 influences the level of nociceptive factors and enhances opioid analgesic potency in neuropathic rats ［J］. J Neuroimmune Pharmacol, 2017, 12(3): 402-419.

［17］Ni H, Yao M, Huang B, et al. Glial activation in the periaqueductal gray promotes descending facilitation of neuropathic pain through the p38 MAPK signaling pathway ［J］. J Neurosci Res, 2016, 94(1): 50-61.

［18］Hu C, Lu K, Liu W. Exendin-4 attenuates inflammation-mediated endothelial cell apoptosis in varicose veins through inhibiting the MAPK-JNK signaling pathway ［J］. J Recept Signal Transduct Res, 2020, 40(5): 464-470.

［19］Wang Y, Xu H, Tao M, et al. Ligustilide relieves complete freund’s adjuvant-induced mechanical hyperalgesia through inhibiting the activation of spinal c-jun n-terminal kinase/c-Jun pathway in rats ［J］. Pharmacogn Mag, 2017, 13(52): 634-638.

［20］Zheng J, Dai Q, Han K, et al. JNK-IN-8, a c-Jun N-terminal kinase inhibitor, improves functional recovery through suppressing neuroinflammation in ischemic stroke［J］. J Cell Physiol, 2020, 235(3): 2792-2799.

［21］Huang X, Peng J, Pang J, et al. Peli1 contributions in microglial activation, neuroinflammatory responses and neurological deficits following experimental subarachnoid hemorrhage ［J］. Front Mol Neurosci, 2017,10:398.

［22］Liu GX, Sun YY, Chen ZL, et al. Effects of inflammatory pain on inflammatory response and expression of TNF-α and MCP-1 in peripheral tissues of mice ［J］. Journal of Xi’an Jiaotong University (Medical Edition), 2019, 40(1): 65-70.(in Chinese)

刘改霞，孙雨瑶，陈子璐，等. 炎性疼痛对小鼠外周组织炎症反应及TNF-α和MCP-1表达的影响［J］. 西安交通大学学报(医学版), 2019, 40(1): 65-70.

［23］Song X, Huang Z, Song WB, et al. Attenuation effect of spinal manipulation on neuropathic and postoperative pain through activating endogenous anti-inflammatory cytokine interleukin 10 in rat spinal cord ［J］. J Manipulative Physiol Ther, 2016, 39(1): 42-53.

［24］Lamacchia ZM, Spengler RN, Jaffari M, et al. Perispinal injection of a TNF blocker directed to the brain of rats alleviates the sensory and affective components of chronic constriction injury-induced neuropathic pain ［J］. Brain Behav Immun, 2019, 82: 93-105.

[1]	洪晓敏李三强崔钦奕郑润月杨孟利罗仁利李前辉 . 酒精性肝纤维化核因子κB信号通路与性别的差异 [J]. 解剖学报, 2024, 55(1): 55-61.
[2]	谢惠兰方芳林毅. Chir99021调控Wnt/β-catenin 信号通路抑制大鼠牙髓干细胞成骨诱导分化的机制[J]. 解剖学报, 2024, 55(1): 67-72.
[3]	魏茜茜李超红赵晨露唐家萍刘玉珍. 大鼠颈上神经节中Ⅰ组代谢型谷氨酸受体表达及慢性间歇性低氧对其的影响 [J]. 解剖学报, 2024, 55(1): 3-9.
[4]	袁蕾杨智伟杨惠刘政海何洁万炜. 三七总皂苷抑制小鼠星形胶质细胞活化缓解完全弗氏佐剂所致炎性痛 [J]. 解剖学报, 2024, 55(1): 25-31.
[5]	马婷婷陈建红刘爱翠李海宁. 抑制lncRNA TUG1下调核苷酸结合寡聚结构域样受体蛋白1炎症小体在延缓阿尔茨海默病进展的作用 [J]. 解剖学报, 2024, 55(1): 32-42.
[6]	邹成功冯浩陈兵唐辉邵川孙谋杨荣何家全. 创伤性颅脑损伤中神经功能障碍与认知功能损伤的动态变化 [J]. 解剖学报, 2024, 55(1): 43-48.
[7]	吕海燕沈西雅赵富生朱梅. 松茸多糖对 1-甲基-4-苯基-吡啶离子诱导 PC12 细胞损伤的影响 [J]. 解剖学报, 2024, 55(1): 49-54.
[8]	郑丽平刘霞杜晓华吴亚娟刘珊珊 . 脑源性神经营养因子在牦牛与黄牛端脑不同区域的表达与分布 [J]. 解剖学报, 2024, 55(1): 10-16.
[9]	苏芃王兴仪梁景岩熊天庆. 一种低密度及高纯度胎鼠原代海马神经元培养方法的优化及鉴定[J]. 解剖学报, 2024, 55(1): 113-119.
[10]	郝永明郭磊周程辉裴汉军李莉赵哲 . 改良腹主动脉缩窄法建立心肌肥厚模型[J]. 解剖学报, 2024, 55(1): 120-124.
[11]	许亚平余悦欣陈金福王柯柯郭志坤 . 高龄大鼠心室肌间质弹性纤维和胶原纤维的结构分布特征及其机制 [J]. 解剖学报, 2023, 54(6): 716-721.
[12]	刘丽平朱梦妮徐慧 . 白细胞介素6对脂多糖诱导所致类子痫前期大鼠有核红细胞的影响 [J]. 解剖学报, 2023, 54(6): 722-729.
[13]	赵卫明李晓余国营王改平常翠芳 . 丝氨酸肽酶抑制因子Kazal型1对肝癌细胞增殖的影响及其机制 [J]. 解剖学报, 2023, 54(6): 695-702.
[14]	王文娟丁雨桐苏昊任捷孙艳荣王涵菲陆嘉莉张林倩白钰秦丽华. 低雌激素状态下大鼠海马及纹状体Nogo-A的表达变化[J]. 解剖学报, 2023, 54(6): 620-627.
[15]	张琴杨俊霞蒋青松. 福辛普利对糖尿病视网膜病变小鼠血管紧张素转化酶2和血管内皮生长因子的影响[J]. 解剖学报, 2023, 54(6): 628-634.