青海汉族妊娠期高血压疾病白细胞介素10基因启动子区-592A/C多态性分子特征

doi:10.16098/j.issn.0529-1356.2023.01.009

摘要/Abstract

摘要：

目的探讨白细胞介素10（IL-10）基因启动子区-592A/C（rs1800872）多态性与青海汉族妇女妊娠期高血压疾病（HDP）的相关性，并确定该基因在HDP组和健康对照组中的表达情况。方法选择青海省HDP患者（HDP组）和正常妊娠妇女（对照组）各140例，以血液DNA为模板，应用限制性内切酶片段长度多态性聚合酶链反应（PCR-RFLP）方法检测HDP组和对照组 IL-10-592A/C多态性分型并测序验证。应用Real-time PCR检测两组胎盘组织中IL-10 mRNA的表达。应用ELISA检测两组血浆IL-10水平。结果 HDP组和对照组IL-10基因AA、AC、CC基因型频率分别为24.29%、44.29%、31.42% 和13.57%、41.43%、45.00% ，两组基因型分布差异有统计学意义（P<0.05）；AA基因型频率HDP组（24.29%）高于对照组（13.57 %）（P<0.05），CC基因型频率HDP组（31.42%）低于对照组（45.00%)（P<0.05），而AC基因型频率两组间差异无统计学意义（P>0.05）；IL-10-592A/C多态性A、C等位基因频率两组间分布有差异，HDP组A等位基因频率高于对照组（χ²=8.079，P<0.05）；HDP组胎盘组织中IL-10 mRNA表达量低于对照组（P<0.01）；HDP组血浆IL-10水平（1.53 ± 0.68）ng/L低于对照组（1.79 ± 0.72）ng/L（P<0.01）。结论 IL-10-592A/C多态性与青海汉族HDP相关；IL-10-592A/C多态性中A等位基因可能通过调节IL-10的表达参与HDP的遗传易感性。

关键词:

妊娠期高血压疾病, 白细胞介素10, 基因多态性, 细胞因子, 实时定量聚合酶链反应, 限制性内切酶片段长度多态性聚合酶链反应, 孕妇

Abstract:

Objective To investigate the association between the interleukin 10 (IL-10) gene promoter region-592A/C (rs1800872) polymorphism and hypertensive disorders of pregnancy (HDP) in Han women of Qinghai province and to determine the expression of this gene in two groups (HDP group and healthy control group) preliminarily. Methods A total of 140 HDP patients (HDP group) and 140 normal pregnant women (control group) in Qinghai Province were selected. Using blood DNA as template, the IL-10-592A/C polymorphism typing of HDP group and control group was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and verified by sequencing. The expression of IL-10mRNA in the placental tissues of the two groups was detected by Real-time PCR. Plasma IL-10 levels of the two groups were detected by ELISA. Results The frequencies of AA, AC and CC genotypes of IL-10 gene in HDP group and control group were 24.29%, 44.29%, 31.42% and 13.57%, 41.43%, 45.00% respectively, the difference in genotype distribution between the two groups was statistically significant (P<0.05);AA genotype frequency in HDP group(24.29%)was higher than that of control group(13.57%)( P<0.05), CC genotype frequency in HDP group (31.42%) was lower than that in control group (45.00%) (P<0.05), while there was no significant difference in genotype frequency of AC between the two groups (P<0.05); The distribution of A and C allele frequencies of IL-10-592A/C polymorphism was different between the two groups, and the A allele frequency of HDP group was higher than that of control group (χ²=8.079, P<0.05); The expression of IL-10mRNA in the placental tissue of the HDP group was lower than that of the control group (P<0.01); The level of plasma IL-10 in HDP group (1.53 ± 0.68) ng/L was lower than that in control group (1.79 ± 0.72)ng/L (P<0.01). Conclusion IL-10-592A/C polymorphism is associated with HDP in Qinghai Han nationality. The A allele in IL-10-592A/C polymorphism may be involved in the genetic susceptibility to HDP by regulating the expression of IL-10.

Key words:

Hypertensive disorders of pregnancy, Interleukin 10, Genetic polymorphism, Cytokine, Real-time PCR, Polymerase chain reaction-restriction fragment length polymorphism, Pregnant woman

中图分类号:

段倩李建华李文佳王茹武震袁明. 青海汉族妊娠期高血压疾病白细胞介素10基因启动子区-592A/C多态性分子特征[J]. 解剖学报, 2023, 54(1): 63-69.

DUAN Qian LI Jian-hua LI Wen-jia WANG Ru WU Zhen YUAN Ming.

Molecular analysis of interleukin 10 promoter region-592A/C polymorphism in hypertensive disorders of pregnancy in Qinghai Han nationality

[J]. Acta Anatomica Sinica, 2023, 54(1): 63-69.

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