羟基类固醇脱氢酶基因家族单核苷酸多态性与指长比的关联性

doi:10.16098/j.issn.0529-1356.2023.02.012

解剖学报 ›› 2023, Vol. 54 ›› Issue (2): 208-215.doi: 10.16098/j.issn.0529-1356.2023.02.012

羟基类固醇脱氢酶基因家族单核苷酸多态性与指长比的关联性

李帆 ^1,2 马占兵 ^1,2 张静^1,2 杨梦怡^1,2 王媛^1,2 马茜^1,2 陆宏 ^1,2 霍正浩 ^1,2 党洁^1,2*

1.宁夏医科大学基础医学院，银川 750004; 2.宁夏医科大学生育力保持教育部重点实验室，银川 750004

收稿日期:2021-10-31 修回日期:2022-02-08 出版日期:2023-04-06 发布日期:2023-04-06
通讯作者: 党洁 E-mail:djjune1978@126.com
基金资助:
宁夏自然科学基金项目;国家自然科学基金项目

Association between hydroxysteroid dehydrogenases gene family single-nucleotide polymorphisms and digit ratio

LI Fan^1,2 MA Zhan-bing ^1,2 ZHANG Jing ^1,2 YANG Meng-yi ^1,2 WANG Yuan ^1,2 MA Qian ^1,2 LU Hong ^1,2 HUO Zheng-hao^1,2 DANG Jie ^1,2*

1.School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China; 2.Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, Ningxia Medical University, Yinchuan 750004, China

Received:2021-10-31 Revised:2022-02-08 Online:2023-04-06 Published:2023-04-06
Contact: DANG Jie E-mail:djjune1978@126.com

摘要/Abstract

摘要：

目的探讨羟基类固醇脱氢酶基因家族8个单核苷酸多态性（SNPs）与人类指长比（2D∶4D）的关联性。方法随机选取808名在校大学生（男性400名，女性408名）为研究对象，拍摄双手掌面照片后利用电脑图像软件测量并计算左、右手各指长比；多重PCR法对11β-羟基类固醇脱氢酶（HSD11B）和17β-羟基类固醇脱氢酶（HSD17B）基因家族8个SNPs位点（rs1000283，rs2236903，rs5479，rs56303414，rs676387，rs4445895，rs2066474，rs8190478）进行基因分型；单因素方差分析法分析不同基因型与2D∶4D的关联。结果宁夏大学生女性左手（L）2D ∶3D，L2D ∶4D、L3D ∶4D、右手（R）2D ∶4D、R2D ∶5D均显著高于男性（P<0.05）；rs2236903（HSD11B1）的基因型频率在男女间的差异具有统计学意义（P<0.05）；HSD11B1基因的rs1000283-rs2236903及HSD11B2基因rs5479-rs56303414存在强关联，但其频率在男女两性间差异均无统计学意义（P >0.05）；不论男性还是女性，8个SNPs位点基因型频率与指长比（2D ∶4D）无显著关联（P>0.05）。结论宁夏汉族大学生群体的指长比性别差异存在显著性，然而，其与HSD11B和HSD17B基因家族8个SNPs位点基因多态性无关联性，提示其可能与人类指长比的形成无关。

关键词: 羟基类固醇脱氢酶, 单核苷酸多态性, 指长比, 宁夏地区, 多重聚合酶链反应, 人

Abstract:

Objective investigate the association between 8 single-nucleotide polymorphisms (SNPs) of hydroxysteroid dehydrogenase (HSD) gene family and human digit ratio (2D ∶4D). Methods Randomly selected 808 college students (400 males and 408 females) as subjects, the digit ratio of left and right fingers were measured and calculated using computer image software. Eight SNPs (rs1000283, rs2236903, rs5479, rs56303414, rs676387, rs4445895, rs2066474, rs8190478) in HSD11B and HSD17B gene families were genotyped by multiplex PCR. The association between 2D∶4D and different genotypes was analyzed by One-Way ANOVA. Results Female left hand(L)2D ∶3D, L2D ∶4D, L3D ∶4D, right hand(R)2D ∶4D, R2D ∶5D were significantly higher than male (P<0.05). The genotype frequency of rs2236903 (HSD11B1) was significantly different between male and female (P <0.05). A good linkage disequilibrium was found between rs1000283and rs2236903 of HSD11B1 gene, rs5479 and rs56303414 of HSD11B2 gene, but there was no statistical difference in frequency between male and female students(P>0.05). The genotypes frequency of the 8 SNPs were not significantly associated with digit ratio (2D ∶4D) in both males and females (P >0.05). Conclusion There are significant gender differences in digit ratio in Ningxia Han college students, but there is no correlation between digit ratio and 8 SNPs in HSD11B and HSD17B gene families, suggesting that HSD11B and HSD17B gene families may have nothing to do with the formation of human digit ratio.

Key words: Hydroxysteroid dehydrogenase, Single-nucleotide polymorphism, Digit ratio, Ningxia region, Multiplex PCR, Human

中图分类号:

Q983

李帆马占兵张静杨梦怡王媛马茜陆宏霍正浩党洁 . 羟基类固醇脱氢酶基因家族单核苷酸多态性与指长比的关联性[J]. 解剖学报, 2023, 54(2): 208-215.

LI Fan MA Zhan-bing ZHANG Jing YANG Meng-yi WANG Yuan MA Qian LU Hong HUO Zheng-hao DANG Jie . Association between hydroxysteroid dehydrogenases gene family single-nucleotide polymorphisms and digit ratio[J]. Acta Anatomica Sinica, 2023, 54(2): 208-215.

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羟基类固醇脱氢酶基因家族单核苷酸多态性与指长比的关联性

Association between hydroxysteroid dehydrogenases gene family single-nucleotide polymorphisms and digit ratio

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