中年男性精子DNA碎片率对体外受精-胚胎移植妊娠结局的影响

doi:10.16098/j.issn.0529-1356.2024.03.013

解剖学报 ›› 2024, Vol. 55 ›› Issue (3): 345-348.doi: 10.16098/j.issn.0529-1356.2024.03.013

• 组织学胚胎学发育生物学 • 上一篇下一篇

中年男性精子DNA碎片率对体外受精-胚胎移植妊娠结局的影响

王平王静陈先兵陈曦*

北京大学人民医院妇产科生殖医学中心，北京 100044

收稿日期:2024-02-28 修回日期:2024-03-17 出版日期:2024-06-06 发布日期:2024-06-11
通讯作者: 陈曦 E-mail:chenxi@pkuph.edu.cn

Effect of sperm DNA fragmentation on in vitro fertilization-embryo transfer outcome among middle-aged men

WANG Ping WANG Jing CHEN Xian-bing CHEN Xi*

Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing 100044, China

Received:2024-02-28 Revised:2024-03-17 Online:2024-06-06 Published:2024-06-11
Contact: CHEN Xi E-mail:chenxi@pkuph.edu.cn

摘要/Abstract

摘要：

目的探讨中年男性精子DNA碎片率（DFI）与精液质量和体外受精胚胎移植妊娠结局的关系。方法共收集180例接受常规体外受精胚胎移植治疗且男性年龄>38岁的精液标本。根据DFI的阈值分成（<30%和≥30%）两组。主要测量指标包括：常规精液参数、激素水平、DFI、受精率、优质胚胎率、临床妊娠率等。结果通过比较分析发现，DFI与男性卵泡刺激素(FSH)、精子活力、精子形态密切相关，且精子活力随着DFI水平的升高而下降（P<0.05）；当DFI≥30%时，优质胚胎率下降（P<0.05），但两组的临床妊娠率差异无显著性（P>0.05）。结论 DFI可以作为中年男性精液常规分析的重要参考指标，虽然DFI影响优质胚胎率，但与辅助生殖治疗的临床妊娠结局无关。

关键词: 精子DNA碎片率, 精子活力, 精子染色质扩散, 形态学观察, 人

Abstract:

Objective To explore the relationship between sperm DNA fragmentation index(DFI) and sperm quality, as well as in vitro fertilization-embryo transfer (IVF-ET) preganancy outcomes in middle-aged men. Methods A total of 180 semen specimens from males aged over 38 years who received comventional IVF-ET treatment were collected and divided into two groups(<30% and ≥30%) based on the DIF threshold. Main measures result including: normal semen parameters, hormone levels, DFI, fertilization rate, good quality embryo rate and pregnancy rate. Results DFI was closely related to male follicle-stimulating hormone（FSH）, sperm motility，morphology, and the sperm vitality decreased with increasing DFI level (P<0.05). When DFI≥30%, the rate of high-quality embryos decreased (P<0.05), but there was no significant difference in the pregnancy rate (P>0.05). Conclusion DFI can serve as an important indicator for routine analysis of semen in middle-aged men. DFI affects the rate of high-quality embryos, but it is not related to the clinical pregnancy outcome of assistant reproduction technology (ART).

Key words: DNA fragmentation index, Sperm motility, Sperm chromatin dispersion, Morphological observation, Human

中图分类号:

R714.8

王平王静陈先兵陈曦. 中年男性精子DNA碎片率对体外受精-胚胎移植妊娠结局的影响[J]. 解剖学报, 2024, 55(3): 345-348.

WANG Ping WANG Jing CHEN Xian-bing CHEN Xi. Effect of sperm DNA fragmentation on in vitro fertilization-embryo transfer outcome among middle-aged men[J]. Acta Anatomica Sinica, 2024, 55(3): 345-348.

参考文献

［1］ Agarwal A, Parekh N, Selvam MK, et al. Male oxidative stress infertility (MOSI): proposed terminology and clinical practice guidelines for management of idiopathic male infertility［J］.World J Mens Health, 2019, 37(3):296-312.

［2］ Hozyen MM, Hassanen EM, Sayed Azzouz Y, et al. Do different sperm selection techniques have an impact on embryological findings and clinical outcomes of abnormal sperm DNA fragmentation patients compared to normal ones, a retrospective cohort study［J］. Fertil Steril, 2019,112(3):e279.

［3］ Jerre E, Bungum M, Evenson D, et al. Sperm chromatin structure assay high DNA stainability sperm as a marker of early miscarriage after intracytoplasmic sperm injection［J］. Fertil Steril, 2019, 112(1):46-53.e2.

［4］ Tello-Mora P, Hernndez-Cadena L, Pedraza J, et al. Acrosome reaction and chromatin integrity as additional parameters of semen analysis to predict fertilization and blastocyst rates［J］. Reprod Biol Endocrinol, 2018, 16(1):102.

［5］ Ni WH, Agarwal A, Sun YP, et al. Clinical expert consensus on sperm DNA fragment detection［J］. Journal of Reproductive Medicine, 2023, 32(2):170-180.(in Chinese)倪吴花, Agarwal A, 孙莹璞, 等. 精子DNA碎片检测的临床专家共识［J］. 生殖医学杂志, 2023, 32(2):170-180.

［6］ Fernández JL, Muriel L, Goyanes V, et al., Halosperm is an easy, available, and cost-effective alternative for determining sperm DNA fragmentation［J］. Fertil Steril, 2005, 84(4):860.

［7］ Li X, Zhu Y, Zhang C, et al. BDE-209 induces male reproductive toxicity via cell cycle arrest and apoptosis mediated by DNA damage response signaling pathways［J］. Environ Pollut, 2019, 255(Pt 1):113097.

［8］ Nicopoullos J, Vicens-Morton A, Lewis SEM, et al. Novel use of COMET parameters of sperm DNA damage may increase its utility to diagnose male infertility and predict live births following both IVF and ICSI［J］. Hum Reprod, 2019, 34(10):1915-1923.

［9］ Evenson, DP. The Sperm Chromatin Structure Assay (SCSA((R))) and other sperm DNA fragmentation tests for evaluation of sperm nuclear DNA integrity as related to fertility［J］. Anim Reprod Sci, 2016, 169:56-75.

［10］ Simon L, Emery B, Carrell DT. Sperm DNA fragmentation: consequences for reproduction［J］. Adv Exp Med Biol,2019,1166:87-105.

［11］ Petersen CG, Mauri AL, Vagnini LD, et al. The effects of male age on sperm DNA damage: an evaluation of 2,178 semen samples［J］. JBRA Assisted Reproduction, 2018,22 (4):323-330.

［12］ Simon L, Aston KI, Emery BR, et al. Sperm DNA damage output parameters measured by the alkaline Comet assay and their importance［J］. Andrologia,2017,49(2):e12608.

［13］ Minhas S, Bettocchi C, Boeri L, et al. European association of urology guidelines on male sexual and reproductive health:2021 update on male infertility［J］. Eur Urol, 2021, 80(5):603-620.

［14］ Simon L, Zini A, Dyachenko A, et al. A systematic review and metaanalysis to determine the effect of sperm DNA damage on in vitro fertilization and intracytoplasmic sperm injection outcome［J］. Asian J Androl, 2017,19(1):80-90.

［15］ Carlini T, Paoli D, Pelloni M, et al. Sperm DNA fragmentation in Italian couples with recurrent pregnancy loss［J］. Reprod Biomed Online, 2017,34(1):58-65.

［16］ Uppangala S, Pudakalakatti F, D’Souza F, et al. Influence of sperm DNA damage on human preimplantation embryo metabolism［J］. Reprod Biol, 2016, 16(3): 234-241.

［17］ Fernandez-Diez C, Gonzalez-Rojo S, Lombo M, et al. Impact of sperm DNA damage and oocyte-repairing capacity on trout development［J］. Reproduction, 2016, 152(1):57-67.

［18］ Sastre D, da Costa NN, de Sa AL, et al. Expression of PLIN2 and PLIN3 during oocyte maturation and early embryo development in cattle［J］. Theriogenology, 2014, 81(2):326-331.

［19］ Nasr-Esfahani MH, Salehi M, Razavi S, et al. Effect of sperm DNA damage and sperm protamine deficiency on fertilization and embryo development post-ICSI［J］. Reprod Biomed Online, 2005,11(2):198-205.

［20］ Tesarik J, Greco E, Mendoza C. Late, but not early, paternal effect on human embryo development is related to sperm DNA fragmentation［J］. Hum Reprod, 2004, 19(3):611-615.

[1]	爼高钰李凤娇咸伟伟过旸洋赵百成李文生尤琳雅. 人正常和帕金森病基底神经节的转录差异分析[J]. 解剖学报, 2024, 55(4): 482-492.
[2]	杜鹃米世雄靳玉川杨倩马敏赵雪汝刘风藏赵长义张展翅樊平崔慧先. 河北医科大学人脑组织库样本分析[J]. 解剖学报, 2024, 55(4): 437-444.
[3]	孙晨曦刘天赐尹常青刘树伟. 磁共振三维伪连续动脉自旋标记技术分析初诊早发型抑郁症患者脑血流灌注[J]. 解剖学报, 2024, 55(4): 493-500.
[4]	黄辰烨李祥君谢道俊. 衰老过程中沿血管周围间隙弥散张量成像分析指数的改变[J]. 解剖学报, 2024, 55(4): 501-507.
[5]	黄辰烨李祥君谢道俊. 脑结构协变网络鲁棒性在不同昼夜节律偏好人群中的差异[J]. 解剖学报, 2024, 55(4): 508-514.
[6]	孙思雨李咏兰郑连斌宇克莉 . 甘南藏族人体组成成分分析[J]. 解剖学报, 2024, 55(3): 349-355.
[7]	希露格于会新廉祎伊拉娜韩瑗李咏兰. 鄂温克族、达斡尔族及蒙古族布里亚特部成人的体成分特征与肥胖[J]. 解剖学报, 2024, 55(3): 356-362.
[8]	钟伟兴王志宏李俊桦廖立青谌祖江李义凯. 强直性脊柱炎征象的脊柱标本观察及影像学分析[J]. 解剖学报, 2024, 55(3): 329-333.
[9]	李明慧熊吉祥周鑫张磊. 数字优化设计结合3D打印技术在下胫腓联合损伤中骨隧道建立的应用[J]. 解剖学报, 2024, 55(3): 334-338.
[10]	苏宝科王永清康志杰王海燕金凤李筱贺曹振华霍佳萱祝勇李峰 . 强直性脊柱炎椎骨去松质骨化截骨术后钛棒有限元分析[J]. 解剖学报, 2024, 55(3): 339-344.
[11]	程全成丁慧如王子元方金玉张晓丽张卫光. 水通道蛋白9商品化抗体和自制抗体识别位点的分析和应用[J]. 解剖学报, 2024, 55(2): 237-240.
[12]	郝经伟潘婷李月朱文斌段文博刘立琨岳丽玲刘云龙高秀丽. 乳腺癌核仁磷蛋白乙酰化及其修饰位点突变体的构建和表达[J]. 解剖学报, 2024, 55(2): 196-202.
[13]	任健陈晓燕. 慢病毒载体介导的类固醇生成因子-1沉默调控子宫内膜基质细胞蜕膜化与自噬[J]. 解剖学报, 2024, 55(2): 188-195.
[14]	司丽娜罗金伟吴迪乔跃兵吕永明徐丛. 关节镜下肩袖缝线桥缝合术后疼痛的原因分析[J]. 解剖学报, 2024, 55(2): 210-214.
[15]	刘晓丽许琳吴爱雪闻彩云李如画吴安婷陈黛茜陈成春. 多发性硬化和视神经脊髓炎的脑灰质结构分析 [J]. 解剖学报, 2024, 55(1): 17-24.

中年男性精子DNA碎片率对体外受精-胚胎移植妊娠结局的影响

Effect of sperm DNA fragmentation on in vitro fertilization-embryo transfer outcome among middle-aged men

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价