解剖学报

解剖学报 ›› 2014, Vol. 45 ›› Issue (5): 585-590.doi: 10.3969/j.issn.0529-1356.2014.05.001

• 神经生物学 •    下一篇

Jagged1对海马放射状胶质细胞增殖和向神经元分化的影响

秦建兵 成敏 金国华* 李浩明 施金洪 邹琳清 田美玲   

  1. 南通大学医学院人体解剖学系, 江苏省神经再生重点实验室，江苏 南通 226001
  • 收稿日期:2014-02-10 修回日期:2014-05-19 出版日期:2014-10-06 发布日期:2013-10-06
  • 通讯作者: 金国华 E-mail:lihaoming@ntu.edu.cn
  • 基金资助:

    国家自然科学基金资助项目;江苏省高校自然科学研究项目;江苏高校优势学科建设工程资助项目

Effects of Jagged1 on hippocampal radial glial cells’proliferation and neuronal differentiation

QIN Jian-bing CHENG Min JIN Guo-hua* LI Hao-ming SHI Jin-hong ZOU Lin-qing TIAN Mei-ling   

  1. Department of Anatomy and Institute of Neurobiology, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Jiangsu Nantong 226001, China
  • Received:2014-02-10 Revised:2014-05-19 Online:2014-10-06 Published:2013-10-06
  • Contact: IN Guo-hua E-mail:lihaoming@ntu.edu.cn

摘要：

目的 探讨Jagged1在体外培养的海马放射状胶质细胞增殖和向神经元分化中的作用。方法 体外培养海马放射状胶质细胞，在培养液中加入Notch信号通路的激动剂Jagged1和(或)抑制剂{氮-［氮-(3,5-二氟苯乙酰)-L-丙氨酰］-S-苯基甘氨酸丁酯，DAPT}，将细胞分为空白对照组、Jagged1组、Jagged1联合DAPT组、DAPT组；细胞计数试剂盒8 (CCK-8)检测各组细胞活力；免疫荧光法检测脑脂结合蛋白(BLBP)/Ki67双标阳性细胞数及分化所得的微管相关蛋白-2(MAP-2)阳性细胞数。
结果 Jagged1组中细胞活力明显高于其他各组，并且Jagged1组中BLBP/Ki67双标阳性细胞及分化所得的MAP-2阳性细胞数也多于其他各组。结论 Jagged1能够促进体外培养的海马放射状胶质细胞增殖，并且能够促进其更多地向神经元分化。

关键词: 放射状胶质细胞, 增殖, 分化, 海马, 神经元, 免疫荧光, 大鼠

Abstract:

Objective To investigate the effect of Jagged1 on hippocampal radial glial cells (RGCs) proliferation and neuronal differentiation in vitro.Methods Hippocampal RGCs were cultured in vitro, the agonist Jagged1 and(or) inhibitor DAPT of Notch signaling were added into the culture medium, and then the cells were divided into control group, Jagged1 group, Jagged1 combined with DAPT group and DAPT group. CCK-8 regent was used to detect cells’ vitality; immunofluorescent was used to detect the number of BLBP/Ki67 double labeled cells and differentiated microtubule associated protein-2(MAP-2) positive cells. Results Cell vitality in Jagged1 group was obviously higher than that of the other groups. The number of BLBP/Ki67 double labeled cells and differentiated MAP-2 positive cells were more than other groups. Conclusion Jagged1 promotes the proliferation and neuronal differentiation of hippocampal RGCs in vitro.

Key words: Radial glial cell, Proliferation, Differentiation, Hippocampus, Neurons, Immunofluorescent, Rat