›› 2009, Vol. 40 ›› Issue (4): 599-603.doi: 10.3969/j.issn.0529-1356.2009.04.016

• 论著 • 上一篇    下一篇

液压转基因技术应用于大鼠再生肝转基因实验

徐存拴1,2*;邢雪琨2,3; 杨献光1,2; 朱秋实2 ; 窦磊2 ; 刘帅帅2 ; 李幼2 ;张富春3   

  1. 1. 河南师范大学生命科学学院,河南新乡 453007;2.河南省科技部共建细胞分化调控重点实验室,新乡 453007;3.新疆大学生命科学与技术学院,乌鲁木齐 830046
  • 收稿日期:2008-08-25 修回日期:2008-10-17 出版日期:2009-08-06
  • 通讯作者: 徐存拴

Hydrodynamics-based transgene directively into rat regenerating liver EM>in vivo/EM>

  1. 1.College of Life Science,He′nan Normal University,He′nan Xinxiang 453007, China; 2.Co-construction Key Laboratory for Cell Differentiation and Regulation, He′nan Xinxiang 453007, China; 3. College of Life Science and Technology,Xin Jiang University,Wulumuqi 830046,China
  • Received:2008-08-25 Revised:2008-10-17 Online:2009-08-06
  • Contact: XU Cun-shuan

关键词: 肝再生, 液压转基因技术, 基因表达, 大鼠

Abstract: Objective To study the conditions and methods of hydrodynamicsbased transgene into rat regenerating liver EM>in vivo/EM>. Methods The solution with concentration 30mg/L genecontaining plasmid was injected into rat tail veins at a speed of 2ml/s, then partial hepatectomy (PH) was performed at different times before/after injection, finally the rat (g) and regenerating liver (g) were weighed, and the liver coefficient (LSUP>c/SUP>) was calculated. Out of 15 groups which are LSUP>c/SUP>±LSUP>c/SUP>*0%, *5%, *10%, *15%, *20%, *25%, *30%, *35%, the most suitable group was chosen as correction coefficient to calculate the most appropriate volume of plasmid solution which was injected into the regenerating liver at different recovery times, and at the same time, right lobe of liver was gathered to make frozen section, then observe and quantify the positive green fluorescent protein (GFP) rate at 488 nm excitation wavelength. Results Injection of either physiological saline or empty plasmid has no significant difference compared with control (only PH performance). The appropriate time of hydrodynamicsbased transgene is more than 12 hours before PH or anytime after PH. The solution volume of hydrodynamicsbased transgene into liver regenerating rat after PH is rat weight (g) ×9%×1/3×corresponding correction coefficient (Trc). Both vector and target gene have effect on the time and abundance of

Key words: Liver regeneration, Hydrodynamicsbased transgene, Gene expression, Rat

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