关键词: 局灶性脑缺血再灌注, 当归补血汤, 免疫组织化学, 大鼠

Abstract: Objective To investigate the protective effect of the cerebral cortex neurons with the decoction of angelicae sinensis for enriching blood on the focal cerebral ischemia-reperfusion rats. Methods Forty-eight male SD rats were randomly divided into sham operation group, middle cerebral artery occlusion and reperfusion group,normal saline control group, decoction-treatment group. Before being operated, treatment-group rats were lavaged for 6 days, twice a day(taking medicine once in the morning and once in the evening ). Four hours after being operated，the rats were lavaged twice each 2.5ml. Rats will be sacrificed the following day.The defects of neurological behavior of the rats and the changes of cerebral infarct volume with TTC staining were also observed; 2% the Evans blue was injected into the femoral vein of rats to observe permeability changes of blood-brain barrier; The numbers and morphological change of neurons in the cerebral cortex were counted through Nissl staining; The p53 and Caspase-3 expression of the neurons in the cerebral cortex were detected with immunohistochemical method; Meanwhile, The apoptotic neurons in the cerebral cortex were observed through terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (TUNEL) staining. Results When compared with the middle cerebral artery occlusion/reperfusion (MCAO) and control saline group, the infarct size in the decoction-treatment group was markedly smaller than that observed in TTC-staining (EM>P/EM><0.05).When brain slices were observed under a fluorescence microscope, less EB spillover was observed in the decoction-treatment group(EM>P/EM><0.05).The number of neurons in the cerebral cortex increased as observed by Nissl-staining(EM>P/EM><0.05). The number of positive immunohistochemistry staining of Caspase-3 and p53 was reduced (P<0.05). The number of apoptosis cell in the cerebral cortex was also reduced (P<0.05). Conclusion The decoction-treatment group could reduce death of neuron in the cerebral cortex. It protects neurons through the reduction of its target genes such as Caspase-3 and p53.

Key words: Focal cerebral ischemia-reperfusion, Decoction of angelicae sinensis for enriching blood, Immunohistochemistry, Rat