解剖学报

›› 2010, Vol. 41 ›› Issue (2): 276-280.doi: 10.3969/j.issn.0529.1356.2010.02.02２

• 论著 • 上一篇    下一篇

低剂量棉酚和甾体激素联合用药对精母细胞凋亡和支持细胞吞噬作用的影响

常青; 钱晓菁 ;许增禄*; 张成侠   

  1. 中国医学科学院基础医学研究所,北京协和医学院基础学院,人体解剖与组织胚胎学系，北京 100005
  • 收稿日期:2009-04-29 修回日期:2009-05-22 出版日期:2010-04-06
  • 通讯作者: 许增禄

Effects of combined administration of low dose gossypol with steroid hormones on spermatocyte apoptosis and phagocytosis of sertoli cells

  1. Department of Anatomy, Histology and Embryology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences， School of Basic Medicine, Peking Union Medical College, Beijing 100005, China
  • Received:2009-04-29 Revised:2009-05-22 Online:2010-04-06
  • Contact: XU Zeng-lu

关键词: 棉酚, 甾体激素, 精母细胞, 吞噬, 体视, 大鼠

Abstract: Objective To investigate effects of combined regimen of low dose gossypol with steroid hormones on apoptosis of spermatocyte and phagocytosis of sertoli cells. Methods Adult male rats were divided into four groups randomly, group GH: rats were fed orally with gossypol(GA) ［12.5mg / (kgI>&#/I>8226;d) ］ and desogestrel(DSG)［125μg/(kgI>&#/I>8226;d) ］/ethinyloestradil(E)［25μg/ (kgI>&#/I>8226;d) ］/testosterone undecanoate(TU)［100mg/(kgI>&#/I>8226;d) ］; group G: a single does of GA［12.5mg/(kgI>&#/I>8226;d ) ］ was given; group H: DSG［125μg/(kgI>&#/I>8226;d) ］/E［25μg/(kgI>&#/I>8226;d) ］/TU［100mg/(kgI>&#/I>8226;d) ］ were administered; group C: rats only received vehicle(1% methyl cellulose). Testes from all the rats were removed at 4, 6 and 8 weeks after treatment for counting of spermatocyte and round spermatid using stereological assay, and for detecting apoptosis of spermatocyte and phagocytosis of sertoli cell by TUNEL and oil red O staining respectively. Results In GH group, the number of spermatocyte and round sperm

Key words: Gossypol, Steroid hormone, Spermatocyte, Phagocytosis, Stereology, Rat 

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