解剖学报

›› 2010, Vol. 41 ›› Issue (6): 779-784.doi: 10.3969/j.issn.0529-1356.2010.06.001

• 论著 •    下一篇

大鼠脊髓源神经干细胞与运动神经元的差异蛋白质组学

王春芳1*; 李鹏飞1 ;蔚洪恩1，2;王菲1 ;韩树峰3 ;王树党1;窦岩1;赵嘉惠1   

  1. 1.山西医科大学生物技术教研室，太原 030001； 2.第二军医大学解剖学教研室， 上海 200433；3.山西医科大学第一附属医院，太原 030001
  • 收稿日期:2009-12-04 修回日期:2010-02-12 出版日期:2010-12-06
  • 通讯作者: 王春芳

Comparison of differential proteomics between neural stem cells and motor neurons in embryonic spinal cord in rats

  1. 1. Department of Biotechnology, Shanxi Medical University, Taiyuan 030001, China; 2. Department of Anatomy, the Second Military Medical University, Shanghai 200433, China; 3. the First Affiliated Hospital, Shanxi Medical University, Taiyuan 030001, China
  • Received:2009-12-04 Revised:2010-02-12 Online:2010-12-06
  • Contact: WANG Chun-fang

关键词: 神经干细胞, 运动神经元, 脊髓, 蛋白质组学, 大鼠

Abstract: Objective To analysis and compare the proteomic differences between neural stem cells and motor neurons in embryonic spinal cord in rat and discover the key different proteins. Methods Separating the protein of cells by the 2-D fluorescence difference gel electrophoresis, and to analyse the differences of protein expression with DeCyder software, and to identify with high performance liquid chromatography-electrospray tandem mass spectrometry. Results About 1 300 protein spots from the cells were gained after gel analysis. Eighty-seven protein spots were selected for mass spectrometry analysis. Fourty-four differently expressed proteins (24 in neural stem cells and 20 in motor neurons) were identified by mass spectrometry analysis. Conclusion Differently expressed proteins between neural stem cells and motor neurons were identified and it is helpful to find the new targets in neural stem cells differentiation into motor neurons.

Key words: Neural stem cell, Motor neuron, Spinal cord, Proteomics, Rat

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