›› 2010, Vol. 41 ›› Issue (6): 847-851.doi: 10.3969/j.issn.0529-1356.2010.06.014

• 论著 • 上一篇    下一篇

特异性下调葡萄糖调节蛋白78降低人涎腺腺样囊性癌侵袭和转移的能力

王宏1;李宏丹 2; 苏荣健2 ;黄克强1*   

  1. 1.辽宁医学院附属第二医院口腔科,辽宁 锦州 121000; 2.辽宁医学院科学实验中心,辽宁 锦州 121001
  • 收稿日期:2010-01-20 修回日期:2010-04-14 出版日期:2010-12-06
  • 通讯作者: 黄克强

P>Specific down-regulation of glucose-regulated protein 78 decreases the invasion and metastasis capability of salivary adenoid cystic carcinoma/P>

  1. 1.Department of Stomatology, the Second Affiliated Hospital of Liaoning Medical University, Liaoning Jinzhou 121000, China;2.Science Laboratory Center, Liaoning Medical University, Liaoning Jinzhou 121001, China
  • Received:2010-01-20 Revised:2010-04-14 Online:2010-12-06
  • Contact: HUANG Ke-qiang1

关键词: 涎腺腺样囊性癌, 葡萄糖调节蛋白78, 转移, 基质金属蛋白酶, 金属蛋白酶组织抑制剂, 小干扰RNA, 免疫印迹法,

Abstract: Objective To explore the effect of specifically down-regulating glucose-regulated protein 78 on invasion and metastasis capability of adenoid cystic carcinoma. Methods Applying small interfering RNA (siRNA) technology to specifically downregulate the expression of GRP78 and analyzing the difference between ACC-2 and si-GRP78 treated ACC-2 by wound healing, Transwell assay, MTT and cytoskeleton staining. Then we detected the effect of down-regulating GRP78 in ACC-2 on matrix metalloproteinases(MMP)-2,MMP-9,tissue inhibitors of metalloproteinase(TIMP)1 and TIMP-2 by Western blotting technology to compare the ACC-2, control siRNA treated ACC-2 and siGRP78 treated ACC-2. Results Wound healing assay revealed down-regulating GRP78 in ACC-2 could inhibit the wound healing. From Transwell assay, we found that the penetrated cells number decreased significantly by down-regulating GRP78. Cytoskeleton staining showed the fibers of si-GRP78 treated ACC-2 were thickening and distinct obviously, and the fibers of ACC-2 and control siRNA treated ACC-2 were thin and blurred. MTT assay showed that down-regulating GRP78 could inhibit 1/3 reproductive ability of ACC-2. Western blotting manifested that down-regulating the expression of GRP78 in ACC-2 decreased the expression of MMP-2, MMP-9 and increased the expression of TIMP-1, TIMP-2. Conclusion Specific down-regulation of GRP78 could inhibit ACC invasi

Key words: Adenoid cystic carcinoma, Glucose-regulated protein 78, Metastasis, Matrix metalloproteinase, Tissue inhibitors of metalloproteinase, siRNA, Western blotting, Human

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