解剖学报

›› 2010, Vol. 41 ›› Issue (6): 909-911.doi: 10.3969/j.issn.0529-1356.2010.06.027

• 论著 • 上一篇    下一篇

大黄素对人胃癌BGC-823细胞体外增殖和迁移的影响

覃红斌1,3; 竹梦2,3; 杜田锴2,3; 佘乾艳3,4; 杨吉安3 ;朱晓玲2，3;魏蕾3 ;张京伟2*   

  1. 1.湖北民族学院医学院组织学胚胎学教研室，湖北 恩施 445000;2.武汉大学中南医院肿瘤科;3.武汉大学基础医学院病理教研室;4.武汉大学人民医院肿瘤科，武汉 430071
  • 收稿日期:2010-03-24 修回日期:2010-05-19 出版日期:2010-12-06
  • 通讯作者: 张京伟

Effects of aloe-emodin on proliferation and migration of human gastric cancer cell line BGC-823

  1. 1.Department of Histology and Embryology College of Medicine,Hubei Institute for Nationalities,Hubei Enshi 445000, China；2.Department of Oncology Zhongnan Hospital of Wuhan University; 3. Department of Pathology College of Medicine, Wuhan University; 4. Department of Oncology Renmin Hospital of Wuhan University, Wuhan 430071, China
  • Received:2010-03-24 Revised:2010-05-19 Online:2010-12-06
  • Contact: ZHANG Jing-wei

关键词: BGC-823细胞, 大黄素, 增殖, 迁移, 四甲基偶氮唑盐检测, 人

Abstract: Objective To investigate the effect of aloe-emodin on proliferation and migration of human gastric cancer cell line BGC-823. Methods The effects of aloe-emodin on cell proliferation,cell cycle stage and migration ability of BGC-823 cells were observed.The proliferation level was measured by MTT method, cell cycle stage was detected with flow cytometry and migration ability was tested by wound healing assay. Results MTT assay showed the inhibitory effect of aloe-emodin on proliferation of BGC-823 cells was dose-dependent; Treatment of BGC-823 cells with aloe-emodin resulted in cell cycle arrest at GSUB>0/SUB>/GSUB>1/SUB> phase by flow cytometry analysis; The migration velocity of BGC-823 cells was obviously inhibited as shown in wound healing assay. Conclusion The inhibitory effect of aloe-emodin on proliferation and migration of BGC-823 cells was dose-dependent, as well as the cell cycle was arrested at GSUB>0/SUB>/GSUB>1/SUB> phase, which offers the clue for further understanding the inhibition mechanism of aloe-emodin on human gastric cancer cell line BGC-823.

Key words: BGC-823 cell, Aloe-emodin, Proliferation, Migration, MTT assay, Human

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