解剖学报

›› 2012, Vol. 43 ›› Issue (6): 807-812.doi: 10.3969/j.issn.0529-1356.2012.06.017

• 组织学胚胎学发育生物学 • 上一篇    下一篇

MAPK/ERK1与STAT3在脂多糖诱导的腺性膀胱炎动物模型中的表达 

黄瑛1* ; 柳达2; 姜罗1; 温昱3; 石玉秀3   

  1. 1.中国医科大学附属盛京医院超声科; 2.中国医科大学附属盛京医院骨科; 3.中国医科大学组织胚胎学教研室， 沈阳 110000
  • 收稿日期:2011-12-26 修回日期:2012-03-02 出版日期:2012-12-06
  • 通讯作者: 黄瑛

Expression of MAPK/ERK1 and STAT3 in a cystitis glandularis modelof rats induced by exposure to lipopolysaccharide 

  1. 1.Department of Ultrasound, Shengjing Hospital of China Medical University, Shenyang 110000, China; 2.Department of Orthopaedic Surgery, Shengjing Hospital of China Medical University, Shenyang 110000, China; 3.Department of Embryology, China Medical University, Liaoning Shenyang 110000, China
  • Received:2011-12-26 Revised:2012-03-02 Online:2012-12-06
  • Contact: HUANG Ying

摘要： 目的 研究丝裂原激活蛋白激酶(MAPK)成员之一ERK1与信号转导子与转录激活子3（STAT3）在内毒素脂多糖(LPS)诱导的大鼠腺性膀胱炎动物模型中的表达。方法 成年雌性SD大鼠24只，建立腺性膀胱炎动物模型，免疫组织化学染色检测上皮组织中ERK1及STAT3的表达，用RT-PCR技术及Western blotting检测ERK1及STAT3基因及蛋白表达量的变化

关键词: 丝裂原激活蛋白激酶, 信号转导子与转录激活子, 脂多糖, 免疫组织化学, 反转录-聚合酶链反应, 大鼠

Abstract: Objective To determine the expression of mitogen activated protein kinase /extracellular signal regulated kinase 1(MAPK/ERK1) and signal transducer and activator of transcription 3(STAT3) in cystitis glandularis (CG) models of rats induced by exposure to lipopolysaccharide (LPS). Methods Twenty-four female Sprague-Dawley rats were used, 10 of which were catheterized and intravesically exposed to LPS for 8 weeks. Immunohistochemistry, RT-PCR and Western blotting methods were used to demonstrate the expression of ERK1 and STAT3 in sections of cystitis glandularis. Results Immunohistochemistry demonstrated that ERK1 and STAT3 were more intensive induced by LPS than those in the control. The relative expression of ERK1 and STAT3 of transcription were 1.99±0.12 and 1.71±0.08, respectively. 〖WTHZ〗Conclusion The increased ERK1 and STAT3 suggest that MAPK/ERK1-STAT signaling pathway may participate the pathological process of CG induced by LPS in r

Key words: Mitogen activated protein kinase, Signal transducer and activator of transcription, Lipopolysaccharide, Immunohistochemistry, RT-PCR, Rat

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