解剖学报

解剖学报 ›› 2014, Vol. 45 ›› Issue (4): 545-549.doi: 10.3969/j.issn.0529-1356.2014.04.019

• 组织学胚胎学发育生物学 • 上一篇    下一篇

神经纤维丝蛋白、突触素在人胚胎脊髓发育阶段的表达

张泳 刘学红*   

  1. 绍兴文理学院医学院，浙江 绍兴 312000
  • 收稿日期:2014-03-06 修回日期:2014-04-19 出版日期:2014-08-06 发布日期:2014-08-06
  • 通讯作者: 刘学红 E-mail:lxh6588@aliyun.com
  • 基金资助:

    浙江省公益性技术应用研究计划项目

Expression of neurofilament protein and synaptophysin in the developing spinal cord of human embryo

ZHANG Yong  LIU Xue-hong*   

  1. School of Medicine, Shaoxing University, Zhejiang Shaoxing 312000, China
  • Received:2014-03-06 Revised:2014-04-19 Online:2014-08-06 Published:2014-08-06
  • Contact: LIU Xue-hong E-mail:lxh6588@aliyun.com

摘要：

目的 探讨神经纤维丝蛋白（NF）、突触素（SYN）在人胚胎脊髓发育阶段的分布规律及其表达意义。方法 应用免疫组织化学法检测第2、3、4个月龄段，共16例人胚胎脊髓中NF、SYN的表达、分布状况。结果 第2个月胚龄时，NF仅在人胚脊髓的前正中裂和后正中隔两侧的边缘层呈弱阳性表达。第3~4月龄时，NF在脊髓的前正中裂、后正中隔及皮质区域均呈阳性或强阳性表达，在室管膜层外侧的部分灰质内可见NF表达阳性的神经纤维。第2～4个月龄段，人胚胎脊髓内均可见SYN阳性表达，随着胎龄的增大，脊髓内SYN阳性表达强度值呈先升高再降低，阳性数量值呈逐月升高。 结论 NF和SYN参与调控人胚胎脊髓组织的生长发育。

关键词: 胚胎, 脊髓, 神经纤维丝蛋白, 突触素, 免疫组织化学, 人

Abstract:

Objective To investigate the neurofilament protein (NF) and synaptophysin(SYN) distribution in the developing stage of human embryonic spinal cord and their clinical significance. Methods Totally 16 of human embryos, aged from 2 to 4 months were used in this study. Immunohistochemical staining was uesd to detect the expression and distribution of NF and SYN in the spinal cord. Results At the second month stage, NF expression was weakly positive in only the marginal layer on both sides of the spinal cord. At 3-4 months, NF expression was positive or strong positive in the spinal cord center crack before and after the middle of partition and cortex. In the ependymal layer of the lateral part of the expression of NF positive nerve fibers were seen in the gray matter. From 2 to 4 month-age, all visible SYN positive expressions distributed in the spinal cord. With the increase of gestational age, SYN positive expression in the spinal cord staining intensity value was increased first, and then decreased. The number of positive values increased month by month. Conclusion NF and SYN both take part in the regulation of human embryonic spinal cord tissue growth.

Key words: Embryo, Spinal cord, Neurofilament protein, Synaptophysin, Immunohistochemistry, Human