Bmi-1在不同发育阶段食管上皮的表达及其意义

›› 2016, Vol. 47 ›› Issue (1): 95-101.

• 组织学胚胎学发育生物学 • 上一篇下一篇

Bmi-1在不同发育阶段食管上皮的表达及其意义

周文斌¹,刘洋²,史苏云¹,陈小雪¹,裴学莲¹,慕晓玲³

1. 新疆石河子大学医学院组织胚胎学教研室
2. 新疆石河子市石河子大学南区医学院人体解剖与组织胚胎教研室
3. 石河子大学医学院组织学胚胎学教研室

收稿日期:2015-08-14 修回日期:2015-11-01 出版日期:2016-02-06 发布日期:2016-02-06
通讯作者: 周文斌 E-mail:dr.zhouwenbin@foxmail.com
基金资助:
中国国家自然科学基金;新疆生产建设兵团科技支疆计划;石河子大学国家级大学生创新创业训练计划

Expression of Bmi-1 in different developmental stage of esophageal epithelium tissues

Received:2015-08-14 Revised:2015-11-01 Online:2016-02-06 Published:2016-02-06
Supported by:
National Natural Science Foundation of China

摘要/Abstract

摘要： 目的：观察PcG 家族蛋白Bmi-1在食管上皮组织中的表达情况，探讨Bmi-1在食管上皮组织发育过程中的表达规律及意义。方法：采用免疫组化法检测120例小鼠胚胎、20例成年小鼠、15例人体胚胎、18例正常成年人食管上皮组织及17例食管癌组织中Bmi-1的表达，并利用图像分析软件对Bmi-1的表达强度进行定量分析，运用统计软件对各组数据进行统计学分析。结果： Bmi-1在各组食管上皮组织及食管癌组织中均存在表达，但各组表达强度不同，差异有统计学意义(P＜0.05)。 Bmi-1在小鼠与人体胚胎时期食管上皮组织中的表达量均高于生后食管上皮组织。 Bmi-1在人体胚胎、正常成年人食管上皮及食管癌组织中表达强度不同，差异有统计学意义(P＜0.01)，并且在食管癌组织中呈强阳性表达。结论：Bmi-1在不同发育阶段食管上皮组织的表达强度不同，与食管上皮组织的发育密切相关。另外，检测Bmi-1的表达对研究食管上皮组织的发育生物学与食管癌的发生发展具有一定意义。

关键词: Bmi-1, 食管上皮组织, 发育, 免疫组织化学

Abstract: Objective To observe the Bmi-1 protein expression in esophageal epithelial tissues ,and to explore the relationship between the expression pattern of Bmi-1 and the development of esophageal epithelial tissues. Methods Immunohistochemistry was used to measure the expression of Bmi-1 in esophageal epithelial tissues of 120 cases mouse in different development time,15 cases human embryos, 18 cases normal adult human and 17 cases human esophageal squamous cell carcinoma (ESCC), then the level of Bmi-1 protein expression in these samples were analyzed by image analysis software, and the statistical analyses were applied to test the relationship between the expression of Bmi-1 and each group. Results Bmi-1 was found to expression in every time of development, but the differences were present in the protein expression level (P＜0.05). Expression of Bmi-1 protein was higher at embryo stage than that after birth both in mice and human. Compared with esophageal tissue of normal adult human, Bmi-1 overexpressed both in human esophageal squamous cell carcinoma and human embryo(P＜0.01). Conclusions The expression of Bmi-1 closely was related with the development of esophageal epithelium.Furthermore,there were a potential significance to detection of the Bmi-1 expression for the research in the developmental biology of esophageal epithelium and the cytobiology of esophageal cancer.

Key words: Bmi-1, esophageal epithelium , development, Immunohistochemistry

周文斌刘洋史苏云陈小雪裴学莲慕晓玲. Bmi-1在不同发育阶段食管上皮的表达及其意义[J]. , 2016, 47(1): 95-101.

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Bmi-1在不同发育阶段食管上皮的表达及其意义

Expression of Bmi-1 in different developmental stage of esophageal epithelium tissues

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