解剖学报

解剖学报 ›› 2017, Vol. 48 ›› Issue (1): 19-24.doi: 10.16098/j.issn.0529-1356.2017.01.004

• 神经生物学 • 上一篇    下一篇

线粒体转录终止因子蛋白家族的表达以及在MPTP / MPP+ 诱导下的表达变化

张林冰 韩艳艳 仇石 许珊 左伋 刘雯*   

  1. 复旦大学上海医学院细胞与遗传医学系, 上海 200032
  • 收稿日期:2016-07-28 修回日期:2016-09-27 出版日期:2017-02-06 发布日期:2017-02-06
  • 通讯作者: 刘雯 E-mail:liuwen@shmu.edu.cn
  • 基金资助:

    上海市科委基础研究重大重点项目

Expression and alteration induced by MPTP / MPP+of mitochondrial transcription termination factors family

ZHANG Lin-bing HAN Yan-yan QIU Shi XU Shan ZUO Ji LIU Wen*   

  1. Department of Cellular and Genetic Medicine, Fudan University,　Shanghai 200032,　China
  • Received:2016-07-28 Revised:2016-09-27 Online:2017-02-06 Published:2017-02-06
  • Contact: LIU Wen* E-mail:liuwen@shmu.edu.cn

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摘要：

目的 探讨线粒体转录终止因子（MTERFs）蛋白家族在组织和细胞中的表达以及在神经毒素MPTP/MPP+诱导下的表达变化。 方法 取正常C57BL小黑鼠的脑、肝、肾组织，以及人神经母细胞瘤细胞SH-SY5Y、人心肌细胞HCM、人肝细胞L-02，检测MTERFs的蛋白表达情况；通过MPTP/MPP+构建帕金森病（PD）动物/细胞模型，检测MTERFs的蛋白表达变化情况。 结果 MTERFs在小鼠脑中和SH-SY5Y细胞中蛋白表达水平较高；MPTP/MPP+ 诱导的PD模型中，MTERF3蛋白表达水平明显降低。 结论 在小鼠脑和SH-SY5Y细胞中，MTERFs表达较高；MPTP/MPP+诱导下MTERF3蛋白表达明显降低，影响了线粒体转录水平，这可能参与了帕金森病发生的过程。

关键词: 线粒体转录终止因子, 1-甲基-4-苯基-1, 2, 3, 6-四氢吡啶/1-甲基-4-苯基吡啶, 帕金森病, 免疫印迹法, 小鼠

Abstract:

Objective To investigate the expression of mitochondrial transcription termination factors (MTERFs) in various tissues and cells, and the changes of MTERFs induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)/1-methyl-4-pheny lpyridine(MPP+ ). Methods Western blotting was used to measure the expression of the MTERFs in brain, liver, kidney of C57BL mice and in human SH-SY5Y, HCM, L-02 cells. MPTP/MPP+ was injected to imitate PD animal/cell models. The changes of MTERFs protein were detected through western blotting. Results The expression of MTERFs was higher in mice brain and SH-SY5Y cells. In PD mouse model induced by MPTP, MTERF3 reduced significantly. Conclusion The expression of MTERFs was higher in mouse brain and SH-SY5Y cells. After induced by MPTP/MPP+ , MTERF3 decreased obviously, which increased transcription level and might involve in a process of Parkinson's disease.

Key words: Mitochondrial transcription termination factor, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine/1-methyl-4-phenylpyridine,  , Parkinson’s disease, Western blotting, Mouse