远端同源异型盒2和叉头盒O3a在大鼠脑皮质发育过程中的分布与表达

doi:10.16098/j.issn.0529-1356.2017.06.004

解剖学报 ›› 2017, Vol. 48 ›› Issue (6): 658-662.doi: 10.16098/j.issn.0529-1356.2017.06.004

远端同源异型盒2和叉头盒O3a在大鼠脑皮质发育过程中的分布与表达

慕雅婷¹李艳² 郭琼^2* 王磊^3*

1.新疆医科大学临床医学院本硕2013级4班，乌鲁木齐 830011;2.基础医学院组织学与胚胎学教研室;3.新疆乌鲁木齐兵团医院创伤科，乌鲁木齐 830011

收稿日期:2017-01-03 修回日期:2017-02-26 出版日期:2017-12-06 发布日期:2017-12-06
通讯作者: 郭琼;王磊 E-mail:2249798964@qq.com
基金资助:
新疆维吾尔自治区自然科学基金医学联合基金

Distal-less homeobox 2/ forkhead box O3a distribution and expression in the development of rat cerebral cortex

MU Ya-ting¹LI Yan²GUO Qiong ^2* WANG Lei ^3*

1. Graode 2013 Class 4 of Master Degree Candidates in Clinical Medicine;2.Department of Histology and Embryology,Xinjiang Medical University, Urumqi 830011, China;3.Department of Traumatology,Bing Tuan Hospital,Urumqi 830011,China

Received:2017-01-03 Revised:2017-02-26 Online:2017-12-06 Published:2017-12-06
Contact: GUO Qiong; WANG Lei E-mail:2249798964@qq.com

摘要/Abstract

摘要：

目的了目的探讨远端同源异型盒2（Dlx2）和叉头盒O3a（FoxO3a）在SD大鼠脑皮质发育过程中的表达特点。方法应用Real-time PCR法检测Dlx2 mRNA和FoxO3a mRNA分别在受孕11d（E11）、E13、E15、E17、E19及生后1d（P1）的表达情况；应用免疫组织化学技术显示Dlx2和FoxO3a在E11、E13、E15、E17、E19及P1蛋白的表达情况。结果 FoxO3a与Dlx2 mRNA在E11～P1均有表达，而FoxO3a mRNA表达高峰明显早于Dlx2 mRNA；Dlx2 mRNA表达在E15～E17时大幅度上升，而在此之后始终保持这一高水平的表达；Dlx2 mRNA与FoxO3a mRNA的表达在E15～P1呈现出一致的变化趋势；Dlx2基因在E19时出现mRNA水平的高表达而未见蛋白表达。结论 Dlx2和FoxO3a基因在胚胎后期鼠脑皮质中均有表达，且其表达趋势具有一定的一致性；两基因的分布随皮质分层而改变。

关键词:

远端同源异型盒2, 叉头盒O3a, 大脑皮质, 胚胎发育, 实时定量聚合酶链反应, 免疫组织化学, 大鼠

Abstract:

Objective To investigate the expression of distal-less homeobox 2(Dlx2) and forkhead box O3a(FoxO3a) in developing cortex of SD rats. Methods Real-time PCR and immunohistochemical staining were performed to study the changes in Dlx2/FoxO3a expression. Results Dlx2/FoxO3a mRNA were expressed in embryonic day 11(E11)-postnatal day 1(P1), while the expression peak of FoxO3a mRNA was significantly earlier than Dlx2 mRNA. The expression of Dlx2 mRNA was significantly increased at E15-E17, and this high level expression was maintained until P1. The expression of Dlx2/FoxO3a mRNA showed a consistent trend in E15-P1. At E19,Dlx2 gene showed high expression of mRNA but no protein expression. Conclusion Dlx2 and Fox03a genes are expressed in the cerebral cortex of the late embryos,and their expression trend is consistent.The distributions of the two genes are changed with the cortical stratification.

Key words:

Distal-less homeobox 2, Forkhead box O3a, Cerebral cortex, Embryo development, Real-time PCR, Immunohistochemistry, Rat

慕雅婷李艳郭琼王磊. 远端同源异型盒2和叉头盒O3a在大鼠脑皮质发育过程中的分布与表达[J]. 解剖学报, 2017, 48(6): 658-662.

MU Ya-ting LI Yan GUO Qiong WANG Lei. Distal-less homeobox 2/ forkhead box O3a distribution and expression in the development of rat cerebral cortex[J]. Acta Anatomica Sinica, 2017, 48(6): 658-662.

参考文献

［1］Merlo GR,Zerega B,Paleari L,et al. Multiple functions of Dlx genes［J］. Int J Dev Biol, 2000, 44(6): 619-626.

［2］Plavicki JS,Squirrell JM,Eliceiri KW,et al. Expression of the Drosophila homeobox gene, Distal-less, supports an ancestral role in neural development［J］. Dev Dyn, 2016, 245(1): 87-95.

［3］Sun H,Liu Z,Li B,et al. Effects of DLX2 overexpression on the osteogenic differentiation of MC3T3-E1 cells［J］. Exp Ther Med, 2015, 9(6): 2173-2179.

［4］Thomas BL,Liu JK,Rubenstein JL,et al. Independent regulation of Dlx2 expression in the epithelium and mesenchyme of the first branchial arch［J］. Development, 2000, 127(2): 217-224.

［5］Mu YT,Gao J,Guo Q,et al. Research on Dlx1 expression in the development of rat cerebral cortex［J］. Chinese Journal of Histochemistry and Cytochemistry, 2016, 25 (1): 49-53.（in Chinese）

慕雅婷，高剑，郭琼，等.大鼠脑皮质发育中Dlx1表达特点研究［J］.中国组织化学与细胞化学杂志，2016,25（1）：49-53.

[6］Nho RS,Hergert P. FoxO3a and disease progression［J］. World J Biol Chem, 2014, 5(3): 346-354.

［7］McClelland Descalzo DL, Satoorian TS, Walker LM, et al. Glucose-induced oxidative stress reduces proliferation in embryonic stem cells via FOXO3A/β-catenin-dependent transcription of p21(cip1) ［J］. Stem Cell Reports, 2016, 7(1): 55-68.

［8］Ikeda JI,Wada N,Nojima S,et al. ID1 upregulation and FoxO3a downregulation by Epstein-Barr virus-encoded LMP1 in Hodgkin’s lymphoma［J］. Mol Clin Oncol, 2016, 5(5):562-566.

［9 Sun G, Hou YB, Jia HY , et al.MiR-370 promotes cell death of liver cancer cells by Akt/FoxO3a signalling pathway［J］.Eur Rev Med Pharmacol Sci, 2016,20(10):2011-2019.

［10］Xu K,Pei H,Zhang Z,et al. FoxO3a mediates glioma cell invasion by regulating MMP9 expression［J］. Oncol Rep, 2016, 36(5):3044-3050.

［11］Padmaja Divya S,Pratheeshkumar P,Son YO,et al. Arsenic induces insulin resistance in mouse adipocytes and myotubes via oxidative stress-regulated mitochondrial Sirt3-FOXO3a signaling pathway［J］. Toxicol Sci, 2015, 146(2):290-300.

［12］Jia Y, Mo SJ, Feng QQ, et al. EPO-dependent activation of PI3K/Akt/FoxO3a signalling mediates neuroprotection in in vitro and in vivo models of Parkinson's disease［J］. J Mol Neurosci, 2014, 53(1):117-124.

［13］Berry M, Hollingworth T.Development of isolated neocortex ［J］.Experientia, 1973, 29 (2):204-207.

［14］Molnar Z,Blakemore C. How do thalamic axon find their way to the cortex?［J］.TINS, 1995, 18(9):389.

［15］Miyagawa K,Saito A,Miyagishi H,et al. Prenatal stress induces vulnerability to stress together with the disruption of central serotonin neurons in mice［J］. Nihon Yakurigaku Zasshi, 2016, 147(4):212-218.

［16］Sun Z,Yan B,Yu WY,et al. Vitexin attenuates acute doxorubicin cardiotoxicity in rats via the suppression of oxidative stress, inflammation and apoptosis and the activation of FOXO3a［J］. Exp Ther Med, 2016, 12(3):1879-1884.

［17］Bruent A,Sweeney LB,Sturgill JF,et al. Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase［J］. Science, 2004, 303(5666):2011-2015.

［18］Kim AD,Kang KA,Piao MJ,et al. Cytoprotective effect of eckol against oxidative stress-induced mitochondrial dysfunction:involvement of the FoxO3a/AMPK pathway［J］. J Cell Biochem, 2014,115(8):1403-1411.

［19］Lu D,Liu J,Jiao J,et al. Transcription factor Foxo3a prevents apoptosis by regulating calcium through the apoptosis repressor with caspase recruitment domain［J］. J Biol Chem, 2013, 288(12): 8491-8504.

［20］Panto MR,Zappala A,Tuorto F,et al. Role ofthe OTXl gene in cell differentiation of manunaliart cortex［J］.Eur J Neurosci, 2004, 19(10):2893-2902.

［21］Sakurai Y,Kurokawa D, Kiyonari H. Otx2 and Otx1 protect diencephalon and mesencephalon from caudalization into metencephalon during early brain regionalization［J］. Dev Biol, 2010, 347(2):392-403.

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远端同源异型盒2和叉头盒O3a在大鼠脑皮质发育过程中的分布与表达

Distal-less homeobox 2/ forkhead box O3a distribution and expression in the development of rat cerebral cortex

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