循环间歇性低氧对大鼠肾上腺β淀粉样前体蛋白裂解酶1表达的影响

doi:10.16098/j.issn.0529-1356.2020.02.002

解剖学报 ›› 2020, Vol. 51 ›› Issue (2): 162-166.doi: 10.16098/j.issn.0529-1356.2020.02.002

循环间歇性低氧对大鼠肾上腺β淀粉样前体蛋白裂解酶1表达的影响

范娅楠李超红贾祥磊刘玉珍*

新乡医学院第一附属医院，河南省神经修复重点实验室，河南省神经病学研究所，河南卫辉 453100

收稿日期:2019-06-26 修回日期:2019-10-08 出版日期:2020-04-06 发布日期:2020-04-06
通讯作者: 刘玉珍 E-mail:yuzhenliu@xxmu.edu.com
基金资助:
国家自然科学基金;新乡医学院研究生科研创新支持计划

Effect of cyclic intermittent hypoxia on the expression of β-site amyloid precursor protein cleaving enzyme 1 in rat adrenal gland

FAN Ya-nan LI Chao-hong JIA Xiang-lei LIU Yu-zhen*

The First Affiliated Hospital of Xinxiang Medical University, He’nan Key Laboratory of Neural Regeneration, He’nan Neurology Institute, He’nan Weihui 453100, China

Received:2019-06-26 Revised:2019-10-08 Online:2020-04-06 Published:2020-04-06
Contact: LIU Yu-zhen E-mail:yuzhenliu@xxmu.edu.com
Supported by:
National Natural Science Foundation of China;Xinxiang Medical University Graduate Research Innovation Support Program

摘要/Abstract

摘要：

目的观察β淀粉样前体蛋白裂解酶1（BACE1）在大鼠肾上腺的表达和定位，检测循环间歇性低氧（CIH）对BACE1表达的影响。方法采用Western blotting和免疫组织化学法检测BACE1在大鼠肾上腺的表达及定位； 16只雄性SD大鼠随机分为2组，常氧对照组（control组）和CIH模型组，每组8只。模型制作2周，Western blotting检测各组大鼠肾上腺髓质BACE1和酪氨酸羟化酶（TH）蛋白的表达量。结果 BACE1主要定位于大鼠肾上腺髓质神经纤维；与control组相比， CIH 组大鼠肾上腺髓质BACE1蛋白水平下降，TH蛋白水平升高（P<0.05）。结论 BACE1定位于大鼠肾上腺髓质神经纤维；BACE1水平下调可能参与减缓CIH引起的交感神经活性过度增强。

关键词: β淀粉样前体蛋白裂解酶1, 肾上腺, 酪氨酸羟化酶, 循环间歇性低氧, 免疫印迹法, 大鼠

Abstract:

Objective To observe the expression and localization of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) in rat adrenal gland and to detect the effect of cyclic intermittent hypoxia (CIH) on the expression of BACE1. Methods The expression and localization of BACE1 in rat adrenal gland were detected by Western blotting and immunohistochemistry. Sixteen male Sprague-Dawley (SD) rats were randomly divided into two groups: control group and CIH group, 8 rats in each group. The protein levels of BACE1 and tyrosine hydroxylase (TH) in rat adrenal medulla were detected by Western blotting after CIH 2 weeks treatment. Results BACE1 was mainly localized in rat adrenal medullary nerve fibers. Compared with the control group, BACE1 protein level decreased and TH protein level increased in the adrenal medulla in the CIH group.

Conclusion BACE1 is located in rat adrenal medullary nerve fibers. The decreased level of BACE1 may participate in slowing down the excessive enhancement of sympathetic activity induced by CIH.

Key words: β-site amyloid precursor protein cleaving enzyme 1, Adrenal gland, Tyrosine hydroxylase, Cyclic intermittent hypoxia, Western blotting, Rat

中图分类号:

范娅楠李超红贾祥磊刘玉珍. 循环间歇性低氧对大鼠肾上腺β淀粉样前体蛋白裂解酶1表达的影响[J]. 解剖学报, 2020, 51(2): 162-166.

FAN Ya-nan LI Chao-hong JIA Xiang-lei LIU Yu-zhen. Effect of cyclic intermittent hypoxia on the expression of β-site amyloid precursor protein cleaving enzyme 1 in rat adrenal gland[J]. Acta Anatomica Sinica, 2020, 51(2): 162-166.

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循环间歇性低氧对大鼠肾上腺β淀粉样前体蛋白裂解酶1表达的影响

Effect of cyclic intermittent hypoxia on the expression of β-site amyloid precursor protein cleaving enzyme 1 in rat adrenal gland

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