广西人群miRNA-107基因多态性分布特征及其与血脂的关系

doi:10.16098/j.issn.0529-1356.2020.02.022

解剖学报 ›› 2020, Vol. 51 ›› Issue (2): 284-288.doi: 10.16098/j.issn.0529-1356.2020.02.022

广西人群miRNA-107基因多态性分布特征及其与血脂的关系

黄艳云^1,2黄华佗¹ 陆玉兰¹ 雷茗¹ 谭昙¹韦叶生^1*

1.右江民族医学院附属医院检验科，广西百色 533000； 2.百色市人民医院检验科，广西百色 533000

收稿日期:2019-01-17 修回日期:2019-03-21 出版日期:2020-04-06 发布日期:2020-04-06
通讯作者: 韦叶生 E-mail:yeshengwei22@163.com
作者简介:2019-06-15
基金资助:
缺血性脑卒中相关的mir-17-92家族基因的筛选和分子机制研究;缺血性脑卒中一个新的microRNA-181家族成员基因的筛选和分子机制研究

Distribution characteristics of polymorphism of miRNA-107 gene in Guangxi population and their association with serum lipid levels

HUANG Yan-yun^1,2 HUANG Hua-tuo¹ LU Yu-lan¹ LEI Ming¹ TAN Tan¹ WEI Ye-sheng^1*

Department of Clinical Laboratory, Affiliated Hospital of Youjiang Medical University for Nationalities, Guangxi Baise 533000,China; 2. Department of Clinical Laboratory, People’s Hospital of Baise, Guangxi Baise 533000, China

Received:2019-01-17 Revised:2019-03-21 Online:2020-04-06 Published:2020-04-06
Contact: WEI Ye-sheng E-mail:yeshengwei22@163.com

摘要/Abstract

摘要：

目的探讨miR-107基因单核苷酸多态性（SNP）位点rs2296616 C/T在广西地区健康人群中的分布特点，对比其在不同种族间基因型及等位基因频率分布的差异，并进一步探讨rs2296616 C/T位点单核苷酸多态性（SNP）与血脂水平的相关性。方法采用多重单碱基延伸SNP分型技术（multiplex SNaPshot）和DNA测序法，检测372例广西健康人rs2296616 C/T位点的多态性，用7600生化仪检测其血脂相关指标，并用统计学方法分别比较rs2296616C/T位点多态性在各种族人群间的分布差异及不同基因型间的血脂水平差异。结果广西人群miR-107基因rs2296616 C/T位点存在TT（91.1%）和CT（8.9%）两种基因型及T（95.6%）和C（4.4%）两种等位基因。该位点的基因型和等位基因型频率在广西人群不同性别间的比较，差异无统计学意义（P>0.05）。其基因型和等位基因频率与人类基因组单体型图（HapMap）所公布的欧洲人、日本人、非洲人、印第安人和墨西哥人分型数据相比较，差异均有统计学意义（P<0.05），但与北京汉族人群比较，差异无统计学意义（P>0.05）。rs2296616 C/T位点两种基因型人群血脂之间比较，携带TT基因型人群的高密度脂蛋白胆固醇（HDL-C）与CT组比较，差异具有统计学意义（P<0.05）。结论广西人群miR-107基因rs2296616 C/T位点多态性与其他种族人群之间比较存在不同程度的差异；rs2296616 C/T位点多态性与HDLC水平高低有关。

关键词: miR-107基因, 单核苷酸多态性, rs2296616 C/T位点, 种族, 血脂, 多重单碱基延伸SNP分型技术, DNA测序法, 广西人群

Abstract:

Objective To study the distribution characteristics of single nucleotide polymorphisms (SNP) of miR-107 gene rs2296616 C/T in Guangxi healthy population and comparison with that in different ethnic populations, and further to explore the correlation between rs2296616 C/T SNP and blood lipid level. Methods The polymorphisms of miR-107 gene rs2296616C/T among 372 Chinese healthy individuals of Guangxi were detected by multiplex SNaPshot and DNA sequencing method , and the blood lipidrelated indexes were detected by 7600 biochemical analyzer. The distribution of rs2296616 C/T polymorphism among different ethnic groups and the differences of blood lipid levels among different genotypes were compared by statistical method . Results MiR-107 gene rs2296616 C/T SNP contained TT（9.1%）, CT（8.9%）genotypes and T（95.6%）, C（4.4%）alleles in Guangxi healthy population. The frequencies of genotype and allele distribution of rs2296616 C/T were not significantly different among genders in Guangxi population（P>0.05）. However, there were significant differences in the genotype and allele frequency of miR-107 gene rs2296616 C/T in Guangxi healthy population compared with those of Europeans, Japanese, Africans, Mexicans and Indians published in HapMap(P<0.05), no significant difference compared with HapMap-HGB(P>0.05). When compared the blood lipid level among two genotypes in rs2296616 C/T, we found that the level of high density lipoprotein cholesterol(HDL-C) with TT genotype was significantly different from that of CT group(P<0.05). Conclusion There are different degrees of variation in the polymorphisms of rs2296616 C/T of miR-107 gene between Guangxi people and other ethnic populations. The polymorphism of rs2296616 C/T locus is related to the level of HDL-C.

Key words: MiR-107 gene, Single nucleotide polymorphism, rs2296616 C/T, Ethnic, Lipoprotein, Multiplex SNaPshot, DNA sequencing, Guangxi population

中图分类号:

R394.3

黄艳云黄华佗陆玉兰雷茗谭昙韦叶生. 广西人群miRNA-107基因多态性分布特征及其与血脂的关系[J]. 解剖学报, 2020, 51(2): 284-288.

HUANG Yan-yun HUANG Hua-tuo LU Yu-lan LEI Ming TAN Tan WEI Ye-sheng. Distribution characteristics of polymorphism of miRNA-107 gene in Guangxi population and their association with serum lipid levels[J]. Acta Anatomica Sinica, 2020, 51(2): 284-288.

参考文献

［1］ Luo Z, Zheng Y, Zhang W. Pleiotropic functions of miR107 in cancer networks［J］. Onco Targets Ther, 2018, 11: 4113-4124.

［2］ Bhatia H, Pattnaik BR, Datta M. Inhibition of mitochondrial beta-oxidation by miR-107 promotes hepatic lipid accumulation and impairs glucose tolerance in vivo［J］. Int J Obes (Lond), 2016, 40(5): 861-869.

［3］ Gao ZF, Ji XL, Gu J, et al. microRNA-107 protects against inflammation and endoplasmic reticulum stress of vascular endothelial cells via KRT1-dependent Notch signaling pathway in a mouse model of coronary atherosclerosis［J］. J Cell Physiol, 2019, 234(7): 12029-12041.

［4］ Altintas O, Ozgen Altintas M, Kumas M, et al. Neuroprotective effect of ischemic preconditioning via modulating the expression of cerebral miRNAs against transient cerebral ischemia in diabetic rats［J］. Neurol Res, 2016, 38(11): 1003-1011.

［5］ Yang ZB, Zhang Z, Li TB, et al. Up-regulation of brain-enriched miR-107 promotes excitatory neurotoxicity through down-regulation of glutamate transporter1 expression following ischaemic stroke［J］. Clin Sci (Lond), 2014, 127(12): 679-689.

［6］ Wang XL, Zhao DX, Zhuang WT, et al. Association between genetic polymorphism and pregnancy induced hypertension in Qinghai ［J］. Acta Anatomica Sinica, 2018, 49 (3): 379-386.(in chinese)

王香林, 赵得雄, 庄文婷, 等. 基因多态性与青海妊娠高血压疾病的相关性［J］. 解剖学报, 2018, 49(3): 379-386.

［7］ Zhu ShSh, Li SF. Association between D6S296, D8S264 polymorphism and schizophrenia of Han/Zhuang population in Guangxi ［J］. Acta Anatomica Sinica, 2014,45 (2): 286-290(in chinese)

朱莎莎, 李松峰. D6S296、D8S264基因座多态性与广西地区汉/壮族人群精神分裂症的关联性［J］. 解剖学报, 2014, 45(2): 286-290.

［8］ Wang S, Lü C, Jin H, et al. A common genetic variation in the promoter of miR-107 is associated with gastric adenocarcinoma susceptibility and survival［J］. Mutat Res, 2014, 769: 35-41.

［9］ Kroliczewski J, Sobolewska A, Lejnowski D, et al. microRNA single polynucleotide polymorphism influences on microRNA biogenesis and mRNA target specificity［J］. Gene, 2018, 640: 66-72.

［10］ Wu J, Jiang R. Prediction of Deleterious Nonsynonymous SingleNucleotide Polymorphism for Human Diseases［J］. Sci World J, 2013, 2013: 675851.

［11］ Locke JM, Lango Allen H, Harries LW. A rare SNP in pre-miR-34a is associated with increased levels of miR-34a in pancreatic beta cells［J］. Acta Diabetol, 2014, 51(2): 325-329.

［12］ Han BW, Li ZH, Liu SF, et al. A comprehensive review of microRNA-related polymorphisms in gastric cancer［J］. Genet Mol Res, 2016, 15(2). doi: 10.4238/gmr.15028289.

［13］ Ghanbari M, Ikram MA, de Looper HW, et al. Genome-wide identification of microRNA-related variants associated with risk of Alzheimer’s disease［J］. Sci Rep, 2016, 6: 28387.

［14］ Wang W, Jiang B, Sun H, et al. Prevalence, incidence, and mortality of stroke in China: results from a nationwide population-based survey of 480 687 adults［J］. Circulation, 2017, 135(8): 759-771.

［15］ Rahman R, Asombang AW, Ibdah JA. Characteristics of gastric cancer in Asia［J］. World J Gastroenterol, 2014, 20(16): 4483-4490.

［16］ Meng S, Cao J, Wang L, et al. MicroRNA 107 partly inhibits endothelial progenitor cells differentiation via HIF-1beta［J］. PLoS One, 2012, 7(7): e40323.

［17］ Li Y, Chang S, Li W, et al. cxcl12-engineered endothelial progenitor cells enhance neurogenesis and angiogenesis after ischemic brain injury in mice［J］. Stem Cell Res Ther, 2018, 9(1):139.

［18］ Ruan C, Shen Y, Chen R, et al. Endothelial progenitor cells and atherosclerosis［J］. Front Biosci (Landmark Ed), 2013, 18: 1194-1201.

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广西人群miRNA-107基因多态性分布特征及其与血脂的关系

Distribution characteristics of polymorphism of miRNA-107 gene in Guangxi population and their association with serum lipid levels

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