神经肌肉电刺激通过调控白细胞介素6/信号转导子及转录激活子3信号通路促进脊髓损伤后小鼠运动功能恢复#br#

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doi:10.16098/j.issn.0529-1356.2024.03.002

解剖学报 ›› 2024, Vol. 55 ›› Issue (3): 260-267.doi: 10.16098/j.issn.0529-1356.2024.03.002

神经肌肉电刺激通过调控白细胞介素6/信号转导子及转录激活子3信号通路促进脊髓损伤后小鼠运动功能恢复#br#
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覃建锋¹ 宋海旺² 孙宝飞² 吉杨丹¹ 龙思方¹ 杨丹^2*

1.黔南民族医学高等专科学校基础医学部，贵州都匀 558003; 2.贵州医科大学基础医学院人体解剖学教研室，贵州贵安新区 561113

收稿日期:2023-05-06 修回日期:2023-07-17 出版日期:2024-06-06 发布日期:2024-06-11
通讯作者: 杨丹 E-mail:stefanie111@126.com
基金资助:
贵州省科技厅计划项目;黔南民族医学高等专科学校科研基金项目;黔南民族医学高等专科学校科研基金项目

Neuromuscular electrical stimulation promoting the recovery of motor function in mice after spinal cord injury by regulating interleukin-6/signal transducer and activator of transcription-3 signaling pathway

QIN Jian-feng¹ SONG Hai-wang² SUN Bao-fei² JI Yang-dan¹ LONG Si-fang¹ YANG Dan^2*

1.School of Basic Medicine, Qiannan Medical College for Nationalities, Guizhou Duyun 558003, China; 2.Department of Human Anatomy, School of Basic Medicine, Guizhou Medical University, Guizhou Gui’an New District 561113, China

Received:2023-05-06 Revised:2023-07-17 Online:2024-06-06 Published:2024-06-11
Contact: YANG Dan E-mail:stefanie111@126.com

摘要/Abstract

摘要：

目的观察神经肌肉电刺激（NMES）对脊髓损伤后小鼠白细胞介素6（IL-6）/STAT3信号通路的影响，探讨其对运动功能恢复的机制。方法选取SPF级小鼠72只随机分成假手术（sham）组、脊髓损伤组（SCI）和NMES组。利用BMS评分、斜坡实验与电生理（EMG）评估小鼠脊髓损伤后肢体运动恢复情况。Western blotting和Real-time PCR检测各组小鼠脊髓组织中相关炎症因子、IL-6/STAT3信号通路与胶质纤维酸性蛋白（GFAP）和脑源性神经营养因子（BDNF）的表达。HE染色观察各组小鼠脊髓形态结构。结果 NMES组BMS评分和小鼠斜坡实验均高于SCI组（P<0.05）；NMES组小鼠运动诱发电位（MEP）最大振幅高于SCI组（P<0.05）；NMES组脊髓组织TNF-α、IL-12A与GFAP表达量均低于SCI组（P<0.05），TGF-β、IL-10与BDNF表达量均高于SCI组（P<0.05）；与SCI组相比，NMES组小鼠脊髓空洞较少，脊髓形态修复较好；与SCI组相比，NMES组IL-6/STAT3信号通路蛋白表达均低于SCI组（P<0.05）。结论神经肌肉电刺激通过抑制IL-6/STAT3信号通路发挥抗炎作用，从而促进脊髓损伤后小鼠后肢运动功能的恢复。

关键词: 脊髓损伤, 炎症反应, 白细胞介素6/信号转导子及转录激活子3信号通路, 神经肌肉电刺激, 免疫印迹法, 小鼠

Abstract:

Objective To observe the effect of neuromuscular electrical stimulation (NMES) on interleukin-6 (IL-6)/STAT3 signaling pathway in mice after spinal cord injury, and to explore the mechanism of its effect on motor function recovery. Methods Seventy-two SPF grade mice were randomly divided into sham operation group, spinal cord injury (SCI) group and NMES group. BMS score, inclined plane test and neuromuscular electrophysiology (EMG) were used to evaluate the recovery of spinal cord injury in mice. Western blotting and Real-time PCR were used to detect the expression of inflammatory factors，IL-6/STAT3, glial fibrillary acidic protein (GFAP)and brain-derived neurotrophic factor (BDNF) in spinal cord tissues of three groups of mice. HE staining was used to observe the pathological changes of spinal cord injury. Results BMS scores and the inclined plane test of mice in the NMES group were higher than those in SCI group (P<0.05). The maximum amplitude of motor evoked potential in NMES group was higher than that in SCI group (P<0.05). The expressions of TNF-α, IL-12A and GFAP in the spinal cord of NMES group were lower than that of SCI group (P<0.05), while the expressions of TGF-β, IL-10 and BDNF were higher than that of SCI group (P<0.05). The protein expressions of IL-6/STAT3 signaling pathway of NMES group were lower than that of SCI group(P<0.05). Conclusion Neuromuscular electrical stimulation plays an anti-inflammatory role by inhibiting the IL-6/STAT3 signaling pathway, thereby promoting the recovery of hind limb motor function in mice after spinal cord injury.

Key words: Spinal cord injury, Inflammation, Interleukin-6/signal transducer and activator of transcription-3 signaling pathway, Neuromuscular electrical stimulation, Western blotting, Mouse

中图分类号:

R322.81

覃建锋宋海旺孙宝飞吉杨丹龙思方杨丹 . 神经肌肉电刺激通过调控白细胞介素6/信号转导子及转录激活子3信号通路促进脊髓损伤后小鼠运动功能恢复#br#

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[J]. 解剖学报, 2024, 55(3): 260-267.

QIN Jian-feng SONG Hai-wang SUN Bao-fei JI Yang-dan LONG Si-fang YANG Dan. Neuromuscular electrical stimulation promoting the recovery of motor function in mice after spinal cord injury by regulating interleukin-6/signal transducer and activator of transcription-3 signaling pathway[J]. Acta Anatomica Sinica, 2024, 55(3): 260-267.

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神经肌肉电刺激通过调控白细胞介素6/信号转导子及转录激活子3信号通路促进脊髓损伤后小鼠运动功能恢复#br#
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Neuromuscular electrical stimulation promoting the recovery of motor function in mice after spinal cord injury by regulating interleukin-6/signal transducer and activator of transcription-3 signaling pathway

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