星形胶质细胞敲除铜蓝蛋白对小鼠脑铁代谢的影响

doi:10.16098/j.issn.0529-1356.2024.04.014

解剖学报 ›› 2024, Vol. 55 ›› Issue (4): 475-481.doi: 10.16098/j.issn.0529-1356.2024.04.014

星形胶质细胞敲除铜蓝蛋白对小鼠脑铁代谢的影响

李海艳^1,2*李忠达²何立娟²

1.承德医学院人体解剖学教研室，河北承德 067000; 2.河北师范大学生命科学学院河北省动物生理生化与分子生物学重点实验室，石家庄 050024

收稿日期:2023-11-13 修回日期:2024-01-24 出版日期:2024-08-06 发布日期:2024-08-06
通讯作者: 李海艳 E-mail:523672654@qq.com
基金资助:
股骨颈数字化模型构建;承德医学院人体解剖与组织胚胎学优势学科资助项目

Effects of conditional knockout ceruloplasmin in astrocytes on brain iron metabolism of mice

LI Hai-yan^1,2* LI Zhong-da² HE Li-juan²

1.Department of Human Anatomy, Chengde Medical College, Hebei Chengde 067000, China； 2.The Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024,China

Received:2023-11-13 Revised:2024-01-24 Online:2024-08-06 Published:2024-08-06
Contact: LI Hai-yan E-mail:523672654@qq.com

摘要/Abstract

摘要：

目的探讨铜蓝蛋白（CP）在脑铁代谢中的作用机制。方法将CP^flox/flox小鼠与GFAP-cre小鼠杂交，得到胶质纤维酸性蛋白（GFAP）-cre介导的CP基因敲除小鼠，即CP^GFAP cKO小鼠。6月龄88只雄性小鼠被分为WT、GFAP-cre、CP^flox/flox和CP^GFAP cKO 4组。通过基因型鉴定、免疫荧光染色和Western blotting方法检测星形胶质细胞是否特异性敲除CP；Morris水迷宫实验检测星形胶质细胞敲除CP的小鼠学习和记忆功能；通过电感耦合等离子体质谱法（ICP-MS）和同步辐射微束X线荧光光谱法（μ-XRF）检测皮层、海马区铁水平；血清和组织铁试剂盒测定血清和肝铁含量。结果基因型鉴定、免疫荧光染色和Western blotting结果确认星形胶质细胞特异性敲除CP基因小鼠建立模型成功；Moris水迷宫实验结果观察到星形胶质细胞特异性敲除CP基因，小鼠的学习记忆能力明显降低；ICP-MS、μ-XRF结果显示，皮层区和海马区铁含量显著下降；而血清铁和肝铁含量没有明显变化。结论星形胶质细胞特异性敲除CP基因的小鼠的学习记忆能力下降与脑铁降低密切相关；CP的主要功能可能是协助脑细胞摄取铁。

关键词: 铜蓝蛋白, 星形胶质细胞, 铁, 认知, 免疫印迹法, 小鼠

Abstract:

Objective To explore the mechanism of ceruloplasmin(CP) in brain iron metabolism. Methods We obtained glial fibrillary acidic protein(GFAP)-cre-mediated ceruloplasmin knockout mice (CP^GFAPcKO mice) by crossing CP^flox/floxmice with transgenic GFAP-cre mice. Eighty-eight male mice aged 6 months were divided into four groups, WT, GFAP-cre, CP^flox/floxand CP^GFAP cKO. To observe whether astrocytic CP gene knockout specifically through genotype identification and immunofluorescent staining, and Western blotting method. The learning and memory function was detected by Morris water maze, brain iron levels in the cortex and hippocampus of mice were measured by inductively coupled plas mamass spectrometry(ICP-MS) and μ-probe X-ray fluorescence(μ-XRF), and iron contents in serum and liver were detected by iron kit. Results The mice of conditional knockout ceruloplasmin in astrocytes were obtained successfully by genotype identification, immunofluorescent staining and Western blotting method. The learning and memory ability was reduced in the water maze experiment, and iron contents decreased in the cortex and hippocampal regions of 6-month-old CP^GFAP cKO group. While the level of serum iron and liver iron in the four groups did not change significantly. Conclusion The decline of learning and memory ability of mice with astrocytic CP gene knockout specifically is closely related to the decrease in brain iron. The main function of CP may be to help brain cells to absorb iron.

Key words: Ceruloplasmin, Astrocyte, Iron, Cognition, Western blotting, Mouse

中图分类号:

Q421

李海艳李忠达何立娟. 星形胶质细胞敲除铜蓝蛋白对小鼠脑铁代谢的影响[J]. 解剖学报, 2024, 55(4): 475-481.

LI Hai-yan LI Zhong-da HE Li-juan. Effects of conditional knockout ceruloplasmin in astrocytes on brain iron metabolism of mice[J]. Acta Anatomica Sinica, 2024, 55(4): 475-481.

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星形胶质细胞敲除铜蓝蛋白对小鼠脑铁代谢的影响

Effects of conditional knockout ceruloplasmin in astrocytes on brain iron metabolism of mice

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