不同发育时期鸡胚脊髓内背侧抑制性轴突导向蛋白的表达和神经嵴细胞迁移之间的相关性

doi:10.3969/j.issn.0529-1356.2013.03.002

解剖学报 ›› 2013, Vol. 44 ›› Issue (3 ): 304-307.doi: 10.3969/j.issn.0529-1356.2013.03.002

不同发育时期鸡胚脊髓内背侧抑制性轴突导向蛋白的表达和神经嵴细胞迁移之间的相关性

苏玉红^1* 张三兵² 崔慧先¹ 康林¹ 王磊¹ 杜鹃¹ 袁世君³

1. 河北医科大学人体解剖学教研室，石家庄 050017; 2.石家庄市第三医院手足外科, 石家庄 050017; 3.邯郸市第一医院神经外二科，河北邯郸 056002

收稿日期:2012-06-05 修回日期:2012-07-10 出版日期:2013-06-06 发布日期:2013-07-16
通讯作者: 苏玉红 E-mail:suyuh1227@hotmail.com
基金资助:
C2011206032；31100789;省自然科学基金资助项目;国家自然科学基金资助项目

Correlation of draxin expression and neural crest migration in chick embryonic spinal cord at different developmental stage

SU Yu-hong ^1* ZHANG San-bing²CUI Hui-xian¹KANG Lin¹ WANG Lei¹ DU Juan¹ YUAN Shi-jun³

1. Department of Anatomy, Hebei Medical University, Shijiazhuang 050017, China; 2. Department of Hand and Foot Surgery，
the Third Hospital of Shijiazhuang City, Shijiazhuang 050017, China; 3. The Second Department of Neurosurgery, the First Hospital of Handan City, Hebei Handan 056002, China

Received:2012-06-05 Revised:2012-07-10 Online:2013-06-06 Published:2013-07-16

摘要/Abstract

摘要：

目的探讨不同发育时期的鸡胚脊髓内背侧抑制性轴突导向蛋白（draxin）表达与神经嵴迁移之间在空间分布上的相关性。方法选
取HH 15-16、HH19-20阶段鸡胚各10只，应用原位杂交、免疫组织化学等方法，观察各阶段鸡胚脊髓内draxin的表达部位及神经嵴细胞迁移路
径，同时比较两者在空间分布上的相关性；应用活组织切片与碱性磷酸酶标记的draxin融合蛋白（draxin-AP）体外孵育的方法，检测迁移路
径内的神经嵴细胞是否具有与draxin蛋白直接结合的能力。结果随着发育时间的推移，鸡胚脊髓内draxin的表达和神经嵴迁移都具有明显的
由颅侧向尾侧渐进性分布的特性，且draxin的表达部位位于神经管背侧、顶板和背侧生皮肌节之间，这些部位恰均位于神经嵴迁移路径的周围；
同时迁移路径内的神经嵴细胞具有与draxin蛋白体外直接结合的能力。结论 draxin表达在神经嵴迁移路径周围且部分神经嵴具有与draxin蛋白
体外直接结合的能力，推测draxin可能在鸡胚脊髓神经嵴迁移过程中发挥重要的调节作用。

关键词: 背侧抑制性轴突导向蛋白, 脊髓, 神经嵴, 迁移, 原位杂交, 免疫组织化学, 鸡胚

Abstract:

Objective To examine the correlation of draxin expression and neural crest migration in chick embryonic spinal
cord at different developmental stages.Methods Ten chick embryos at different stages were used. In situ hybridization and
immunohistochemistry were used to observe the localization of draxin expression, the migration pathway of neural crest cell-the
spatial correlation of draxin expression and the neural crest migration. Living tissue incubation with draxin alkaline phosphatase
(draxin-AP) recombinant protein was used to check the binding ability of migrating neural crest with draxin protein. Results With
development, the draxin expression and neural crest migration had a very similar anterior-posterior gradient in chick embryonic
spinal cord. Draxin was expressed in the dorsal neural tube, roof plate and dorsal tip of dermomyotome which surrounded the
migration pathway of neural crest cells. We also found that portion of migrating neural crest cells bound to draxin-AP protein
directly. Conclusion Draxin is expressed in the tissues surrounding the neural crest migration pathway and portion of migrating
neural crest cells binds to draxin protein directly. We conclude therefore that draxin may be involved in the regulation of neural
crest cell migration in chick embryonic spinal cord.

Key words: Draxin, Spinal cord, Neural crest, Migration, In situ hybridization, Immumohistochemistry, Chick embryo

苏玉红* 张三兵崔慧先康林王磊杜鹃袁世君. 不同发育时期鸡胚脊髓内背侧抑制性轴突导向蛋白的表达和神经嵴细胞迁移之间的相关性[J]. 解剖学报, 2013, 44(3 ): 304-307.

SU Yu-hong* ZHANG San-bing CUI Hui-xian KANG Lin WANG Lei DU Juan YUAN Shi-jun. Correlation of draxin expression and neural crest migration in chick embryonic spinal cord at different developmental stage[J]. AAS, 2013, 44(3 ): 304-307.

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不同发育时期鸡胚脊髓内背侧抑制性轴突导向蛋白的表达和神经嵴细胞迁移之间的相关性

Correlation of draxin expression and neural crest migration in chick embryonic spinal cord at different developmental stage

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