解剖学报 ›› 2013, Vol. 44 ›› Issue (4 ): 509-513.doi: 10.3969/j.issn.0529-1356.2013.04.012

• 肿瘤生物学 • 上一篇    下一篇

14-3-3zeta与p-Bad在T1期非小细胞肺癌中的表达及意义

臧东钰1* 李福智1 杨春雨2   

  1. 1.辽宁医学院附属第三医院胸外科; 2.附属第一医院病理科, 辽宁 锦州 121001
  • 收稿日期:2012-12-04 修回日期:2013-02-07 出版日期:2013-08-06 发布日期:2013-09-04
  • 通讯作者: 臧东钰 E-mail:zdy2351263@sina.com
  • 基金资助:

    省自然科学基金资助项目

Expression of 14-3-3zeta and p-Bad proteins in stage T1 non-small cell lung cancer and the significance

ZANG Dong-yu 1* LI Fu-zhi1 YANG Chun-yu2   

  1. 1.Department of Thoraxes Surgery the Third Affiliated Hospital; 2.Department of Pathology, the First Affiliated Hospital, Liaoning Medical University, Liaoning Jinzhou 121001, China
  • Received:2012-12-04 Revised:2013-02-07 Online:2013-08-06 Published:2013-09-04

摘要:

目的 观察14-3-3zeta 和p-Bad在T1 期非小细胞肺癌 (NSCLC) 中的表达,探讨两者在非小细胞肺癌发展中的作用及相互关系。方法 取110例非小细胞肺癌及癌旁正常肺组织存档蜡块和50例新鲜NSCLC及癌旁正常肺组织标本,采用免疫组织化学和免疫印迹法(Western blotting)
检测14-3-3zeta 和p-Bad 蛋白的表达。结果 与正常肺组织相比,14-3-3zeta 在NSCLC 中的表达显著增强,与肺癌的分化程度和淋巴结转移有关,而与患者的年龄、性别及病理学分型无关。p-Bad在正常肺组织中轻微表达,而在NSCLC 中表达显著增强。且其表达与患者的年龄、性别及
病理学分型无关,但与癌组织分化程度、淋巴结转移相关。结论 14-3-3zeta蛋白与p-Bad与T1期NSCLC发展、侵袭和转移密切相关。

关键词: 非小细胞肺癌, 14-3-3zeta, p-Bad, 免疫印迹法,

Abstract:

Objective To observe the expression of 14-3-3zeta and p-Bad in stage T1 non-small cell lung cancer(NSCLC), and to investigate the relationship between the expression of 14-3-3zeta and p-Bad in NSCLC. Methods One hundred and ten paraffin
blocks and 50 fresh samples from NSCLC patients and normal lung were observed. Expression of 14-3-3zeta and p-Bad were detected by immunohistochemistry and Western blotting. Results Up-regulation of 14-3-3zeta was observed significantly in stage T1 NSCLC
in contrast to that in the normal lung. The increased 14-3-3zeta protein level was correlated with differentiation degree and lymph node metastasis, but it was disassociated with age, gender and histological type. Decreased expression of p-Bad was observed
in NSCLC in contrast to that in the normal lung. Expression of p-Bad was related to differentiation degree and lymph node metastasis, but it was disassociated with age, gender and histological type. Conclusion 14-3-3zeta and p-Bad play an important role in
development and progression of NSCLC.

Key words: Non-small cell lung cancer, 14-3-3zeta, p-Bad, Western blotting, Human

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