孤雌胚胎干细胞来源的诱导多能干细胞的建立及对印记基因表达的影响

doi:10.16098/j.issn.0529-1356.2015.04.019

解剖学报 ›› 2015, Vol. 46 ›› Issue (4): 553-557.doi: 10.16098/j.issn.0529-1356.2015.04.019

• 组织学胚胎学发育生物学 • 上一篇下一篇

孤雌胚胎干细胞来源的诱导多能干细胞的建立及对印记基因表达的影响

单智焱¹ 武玢¹张玥¹ 薛媛¹ 吴嫣爽¹ 沈星辉¹ 雷蕾¹ 刘忠华^2*

1. 哈尔滨医科大学组织学与胚胎学教研室，哈尔滨 150081; 2. 东北农业大学生命科学院，哈尔滨 150081

收稿日期:2014-11-13 修回日期:2014-12-29 出版日期:2015-08-06 发布日期:2015-08-06
通讯作者: 刘忠华 E-mail:liu086@126.com
基金资助:
RNA甲基化修饰对体细胞重编程的调控作用及机制研究

Generation of induced pluripotent stem cells derived from parthenogenetic embryonic stem cells and reprogramming of imprinted genes

SHAN Zhi-yan¹ WU Bin¹ ZHANG Yue¹ XUE Yuan¹ WU Yan-shuang¹ SHEN Xing-hui¹ LEI Lei¹ LIU Zhong-hua ^2*

1. Department of Histology and Embryology, Harbin Medical University, Harbin 150081, China; 2. Life Science, Northeast Agricultural University College, Harbin 150081, China

Received:2014-11-13 Revised:2014-12-29 Online:2015-08-06 Published:2015-08-06
Contact: LIU Zhong-hua E-mail:liu086@126.com

摘要/Abstract

摘要：

目的通过诱导多能干细胞（iPSCs）技术重编程孤雌胚胎干细胞，探讨iPSCs技术对孤雌胚胎干细胞的多能性及印记基因的影响。方法从孤雌激活的囊胚中建立了孤雌胚胎干细胞；利用反转录病毒将多能性转录因子转入孤雌胚胎干细胞中，建立孤雌iPS细胞。结果建立的孤雌来源的iPS细胞体内外分化能力与孤雌胚胎干细胞的差别无显著性；Real-time PCR结果显示，孤雌iPS细胞母源印记基因的表达明显高于孤雌胚胎干细胞，父源印记基因表达下降，多能性基因表达升高。
结论 iPSCs技术能影响基因的表达，尤其是印记基因，印记使其更接近于正常受精来源的胚胎干细胞中印记基因水平。

关键词: 孤雌胚胎干细胞, 诱导多能干细胞, 印记基因, 实时定量聚合酶链反应, 小鼠

Abstract:

Objective To investigate the effect of pluripotency and the expression of imprinted genes on the parthenogenetic embryonic stem cells (PgESCs) by induced pluripotent stem cells (iPSCs) reprogrmming. Methods In our study, we firstly generated the PgESCs from parthenogenetic blastocyst, induced the pluripotent factors into the PgESCs by retrovirus transfection and generated the iPS cells derived from PgESCs. Results It showed no difference in pluripotency and differentiation between PgESCs and PgESC-iPS cells, however, the expression of maternal imprinted genes was significantly increased and the expression of paternal imprinted genes was decreased in PgESC-iPS cells compared to that in PgESCs. Conclusion These results demonstrated the expression of imprinted genes could be modified by iPSCs reprogramming, which may be close to the expression level of imprinted genes in fertilized embronic stem cells.

Key words: arthenogenetic stem cell, Induced pluripotent stem cell, Imprinted gene, Real-time PCR, Mouse

单智焱武玢张玥薛媛吴嫣爽沈星辉雷蕾刘忠华*. 孤雌胚胎干细胞来源的诱导多能干细胞的建立及对印记基因表达的影响[J]. 解剖学报, 2015, 46(4): 553-557.

SHAN Zhi-yan WU Bin ZHANG Yue XUE Yuan WU Yan-shuang SHEN Xing-hui LEI Lei LIU Zhong-hua*. Generation of induced pluripotent stem cells derived from parthenogenetic embryonic stem cells and reprogramming of imprinted genes[J]. AAS, 2015, 46(4): 553-557.

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孤雌胚胎干细胞来源的诱导多能干细胞的建立及对印记基因表达的影响

Generation of induced pluripotent stem cells derived from parthenogenetic embryonic stem cells and reprogramming of imprinted genes

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