膜联蛋白A7低表达对胃癌MGC-803细胞生物学特性的影响

doi:10.16098/j.issn.0529-1356.2015.02.012

解剖学报 ›› 2015, Vol. 46 ›› Issue (2): 216-220.doi: 10.16098/j.issn.0529-1356.2015.02.012

膜联蛋白A7低表达对胃癌MGC-803细胞生物学特性的影响

田焕娜¹王小杰¹陈龙¹梁秀军¹刘兰芳²李欣^1*

1.承德医学院基础医学研究所, 河北承德 067000; 2. 承德医学院附属医院肿瘤内科，河北承德 067000

收稿日期:2014-09-16 修回日期:2014-11-14 出版日期:2015-04-06 发布日期:2015-04-06
通讯作者: 李欣 E-mail:cd-lixin@163.com

Effect of annexin A7 knockdown on biological behaviour of MGC-803 cells

TIAN Huan-na¹ WANG Xiao-jie¹ CHEN Long¹ LIANG Xiu-jun¹LIU Lan-fang²LI Xin 1^*

1. Basic Medical Institute, Chengde Medical College， Hebei Chengde 067000, China;2. Medical Oncology, Affiliated Hospital of Chengde Medical College，Hebei Chengde 067000, China

Received:2014-09-16 Revised:2014-11-14 Online:2015-04-06 Published:2015-04-06
Contact: LI Xin E-mail:cd-lixin@163.com

摘要/Abstract

摘要：

目的探讨膜联蛋白A7(ANXA7)低表达对人胃癌低分化细胞MGC-803的影响。方法将细胞分为实验组、阴性对照组和空白对照组。采用RNA干扰技术将靶向ANXA7的siRNA和阴性对照siRNA以脂质体转染法分别转染胃癌细胞系MGC-803细胞，空白对照组不予任何处理。转染48h后，采用Western blotting 和实时定量-PCR法进行抑制效果的鉴定。检测ANXA7低表达对MGC-803细胞黏附、伸展、剥脱和迁移等生物学行为的影响。结果靶向ANXA7的siRNA可显著抑制ANXA7的表达；ANXA7表达受抑后，细胞黏附、伸展和剥脱能力降低，与阴性对照组和空白对照组相比，差异显著（P<0.05）。细胞迁移变化不显著。结论 ANXA7表达降低后MGC-803细胞行为学发生明显改变。

关键词: 膜联蛋白A7, 细胞运动, MGC-803细胞, 免疫印迹法

Abstract:

Objective To explore the effect of annexin A7(ANXA7) knockdown on biological behaviour of MGC-803 cells. Methods The MGC-803 cells were grouped into experiment group, negative group and blank group. RNA interference technology was used to transfect the siRNA targeted ANXA7, scrambled siRNA into MGC-803 cells with lipofectine 2000 without any treatment to the blank group. After transfection for about 48 hours, Western blotting and Real-time PCR were used to assure the suppression of ANXA7 on both protein level and mRNA level. Cell adhesion assay, detachment assay, and spreading assay and wound healing assay were used to detect the adhesion, detachment, spreading and migration abilities of MGC-803 cells. Results The expression of ANXA7 was significantly suppressed in the cells which were transfected with ANXA7 siRNA. When ANXA7 expression was suppressed, the cell adhesion rate, detachment rate, spreading rate in experiment group were significantly lower than those in negative group and blank group(P<0.05). Compared with the negative group and blank group, migration ability of experiment group was not significantly different. Conclusion The biological behaviour of MGC-803 cells change obviously when ANXA7 expression is suppressed and ANXA7 could become potential target of therapeutic strategies.

Key words: Annexin A7, Cell motility, MGC-803 cell, Western blotting

田焕娜王小杰陈龙梁秀军刘兰芳李欣*. 膜联蛋白A7低表达对胃癌MGC-803细胞生物学特性的影响[J]. 解剖学报, 2015, 46(2): 216-220.

TIAN Huan-na WANG Xiao-jie CHEN Long LIANG Xiu-jun LIU Lan-fang LI Xin*. Effect of annexin A7 knockdown on biological behaviour of MGC-803 cells[J]. AAS, 2015, 46(2): 216-220.

参考文献

［1］Gerke V, Creutz CE, Moss SE. Annexins: linking Ca2+signaling to membrane dynamics［J］. Nat Rev Mol Cell Bio, 2005, 6(6):449-461.
［2］Rescher U, Gerke V. Annexinsunique membrane binding proteins with diverse functions［J］. J Cell Sci, 2004, 117(13):2631-2639.
［3］Fatimathas L, Moss SE. Annexins as disease modifiers［J］. Histol Histopathol, 2010, 25(4):527-532.
［4］Srivastava M, Bubendorf L, Srikantan V, et al. ANX7, a candidate tumor suppressor gene for prostate cancer［J］. Proc Natl Acad Sci USA, 2001, 98(8):4575-4580. 
［5］Srivastava M, Bubendorf L, Raffeld M, et al. Prognostic impact of ANX7-GTPase in metastatic and HER2-negative breast cancer patients［J］. Clin Cancer Res, 2004, 10(7):2344-2350.
［6］Kataoka TR, Ito A, Asada H, et al. Annexin VII as a novel marker for invasive phenotype of malignant melanoma［J］. Jpn J Cancer Res, 2000, 91(1):75-83.
［7］Yadav AK, Renfrow JJ, Scholtens DM, et al. Monosomy of chromosome 10 associated with dysregulation of epidermal growth factor signaling in glioblastomas［J］. JAMA, 2009, 302(3):276-289.
［8］Xi JM, Zhao Q. Expression of ANXA7 in gastric cancer tissues and their effects on the differentiation and metastasis of gastric cancer ［J］. Journal of Clinical and Experimental Medicin, 2010, 9(10):726-727. (in Chiense)
奚剑敏, 赵强. AnnexinA7表达与胃癌分化和转移关系研究［J］. 临床和实验医学杂志, 2010, 9(10):726-727.
［9］Creutz CE, Pazoles CJ, Pollard HB. Identification and purification of an adrenal medullary protein (synexin) that causes calcium-dependent aggregation of isolated chromaffin granules［J］. J Biol Chem, 1978, 253(8):2858-2866. 
［10］Srivastava M, Torosyan Y, Raffeld M, et al. ANXA7 expression represents hormonerelevant tumor suppression indifferent cancers［J］. Int J Cancer, 2007, 121(12): 2628-2636.
［11］Ji H, Moritz RL, Kim YS, et al. Analysis of Ras-induced oncogenic transformation of NIH-3T3 cells using differential display 2-DE proteomics ［J］. Electrophoresis, 2007, 28(12):1997-2008.
［12］Srivastava M, Bubendorf L, Nolan L, et al. ANX7 as a bio-marker in prostate and breast cancer progression［J］. Dis Markers, 2001, 17(2):115-120.
［13］Mussunoor S, Murray GI. The role of annexins in tumour development and progression［J］. J Pathol, 2008, 216(2): 131-140.
［14］Wang XJ, Li X. Influence of annexin A7 low expression on proliferation of HepG2 cells ［J］. Acta Anatomica Sinica, 2013,44(5) :656-660. (in Chiense)
王小杰, 李欣. 膜联蛋白A7低表达对人肝癌HepG2细胞增殖的影响［J］. 解剖学报, 2013, 44(5) :656-660.
［15］Wang ZQ, Tang JW, Wang ShQ, et al. Establishment of mouse hepatic cancer cell line Hca-F with knockdown of ANXA7 gene［J］. Academic Journal of Second Military Medical University, 2008, 29(9):1029-1033. (in Chiense)
王志强, 唐建武, 王绍青, 等. 膜联蛋白A7表达稳定下调的小鼠肝癌Hca-F细胞株的建立［J］. 第二军医大学学报, 2008, 29(9):1029-1033.
［16］Caterino M, Ruoppolo M, Orrù S, et al. Characterization of red cell membrane proteins as a function of red cell density: AnnexinⅦ in different forms of hereditary spherocytosis［J］. FEBS Lett, 2006, 580(28-29):6527-6532.
［17］Huang YH, Asma Saleem qazi, Mohmmed mibrahim, et al. Study of proliferation and invasion activity in different mouse hepatocarcinoma cell lines and location and isoform expression of ANXA7［J］. China Journal of Modern Medicine, 2012, 22(35):9-13. (in Chiense)
黄玉红, Asma saleem qazi, Mohmmed mibrahim, 等. 不同小鼠腹水型肝癌细胞增殖侵袭能力与膜联蛋白A7的亚细胞定位和亚型表达研究［J］. 中国现代医学杂志, 2012, 22(35):9-13.

[1]	徐丽金清东陈秀娇陈超郑伟男周瑞祥. 褪黑素抑制胃癌细胞系SGC-7901的迁移和侵袭[J]. 解剖学报, 2021, 52(5): 751-758.
[2]	廖鸿雁刘杰刘菁郭霜杨琴. 白藜芦醇对氧糖剥夺/再复氧损伤后小胶质细胞系N9活化的影响[J]. 解剖学报, 2019, 50(2): 137-144.
[3]	吴雪艳赵依纳王小杰李欣 . 半乳糖凝集素3低表达对人胃癌MGC-803细胞增殖的影响[J]. 解剖学报, 2018, 49(6): 724-729.
[4]	王小杰赵依纳李欣. 敲低膜联蛋白A5对人胃癌MGC-803和MKN-45细胞周期的影响[J]. 解剖学报, 2018, 49(3): 342-346.
[5]	徐新伟杨玉玲孙志亮张国新曾国栋李洪利尹崇高*. Raptor通过Wnt3a/β-catenin信号通路对乳腺癌侵袭与转移的作用[J]. 解剖学报, 2017, 48(6): 682-687.
[6]	王鑫高俊玲* 赵曼曼朱会兴田艳霞李冉江小华于雷田景瑞崔建忠. 骨髓间充质干细胞抑制转化生长因子-β1诱导的肺泡Ⅱ型上皮细胞间质化[J]. 解剖学报, 2016, 47(6): 756-762.
[7]	张军峰史利利张力赵朝华杨蓬勃张建水刘勇徐曦*. 低氧反应元件介导的低氧调控的神经营养因子-3表达上调减少低氧诱导的PC12细胞凋亡[J]. 解剖学报, 2015, 46(5): 581-586.
[8]	张巍张若文朱文赫李妍罗军赵行宇徐俊杰姜艳霞吕士杰*. 胡桃醌诱导HeLa细胞凋亡的机制[J]. 解剖学报, 2015, 46(5): 641-645.
[9]	张扬许倩陈龙王小杰田焕娜李春辉李欣*. 膜联蛋白A7在胃癌组织中的表达和意义[J]. 解剖学报, 2015, 46(4): 528-531.
[10]	石松林赵振利王国红刘凡路锟杨玲韩荣李晓李祺福. Prohibitin在姜黄素诱导人成骨肉瘤MG-63细胞凋亡过程中的调控机制[J]. 解剖学报, 2015, 46(1): 72-80.
[11]	王小杰梁秀军李欣*. 敲低膜联蛋白A7对人肝癌HepG2细胞Bcl-2和Bax表达的影响[J]. 解剖学报, 2014, 45(6): 809-813.
[12]	桑婷婷胡江江薛建有戚武林赵辅昆张世馥*. NonO蛋白在丁酸钠诱导小鼠红白血病细胞分化过程中的表达[J]. 解剖学报, 2014, 45(4): 516-520.
[13]	黄帅帅王雪庄海慧王宇多周细武王萍*. 转录因子环磷酸腺苷反应元件结合蛋白在紫杉醇诱导HeLa 细胞周期阻滞中的作用及其机制[J]. 解剖学报, 2014, 45(4): 485-492.
[14]	刘景利石新丽崔澂任来峰郑文广李明远*. p53-Ser³⁹²的磷酸化在肿瘤治疗中的作用[J]. 解剖学报, 2014, 45(3): 437-440.
[15]	王小杰李欣* . 膜联蛋白A7低表达对人肝癌HepG2细胞增殖的影响[J]. 解剖学报, 2013, 44(5 ): 656-660.

膜联蛋白A7低表达对胃癌MGC-803细胞生物学特性的影响

Effect of annexin A7 knockdown on biological behaviour of MGC-803 cells

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价