解剖学报

解剖学报 ›› 2021, Vol. 52 ›› Issue (5): 751-758.doi: 10.16098/j.issn.0529-1356.2021.05.012

• 肿瘤生物学 • 上一篇    下一篇

褪黑素抑制胃癌细胞系SGC-7901的迁移和侵袭

徐丽1* 金清东2 陈秀娇1 陈超1 郑伟男1 周瑞祥3*
  

  1. 1.福建省莆田学院基础医学部，福建 莆田 351100; 2.福建省莆田市第一医院神经外科，福建 莆田 351100; 3.福建医科大学人体解剖学与组织学胚胎学系，神经生物学研究中心，福州 350004
  • 收稿日期:2020-07-23 修回日期:2021-01-17 出版日期:2021-10-06 发布日期:2021-10-06
  • 通讯作者: 徐丽；周瑞祥 E-mail:zhourx@mail.fjmu.edu.cn
  • 基金资助:
    褪黑素对胃癌侵袭转移的抑制作用及其分子机制研究;PA28β与mda-7/IL-24在食管癌侵袭转移中的作用及其分子机制研究;褪黑素对胃癌侵袭转移的影响及其机制研究

Melatonin inhibiting the migration and invasion of gastric cancer cell line SGC-7901#br#

XU Li1*  JIN Qing-dong2  CHEN Xiu-jiao1  CHEN Chao1  ZHENG Wei-nan1  ZHOU Rui-xiang3*#br#   

  1. 1. Basic Medical College of Putian University, Fujian Putian 351100, China; 2.Department of Neurosurgery, Putian First Hospital, Fujian Putian 351100, China;   3.Department of Human Anatomy and Histology and Embryology, Neurobiology Research Center, Fujian Medical University, Fuzhou 350004, China
  • Received:2020-07-23 Revised:2021-01-17 Online:2021-10-06 Published:2021-10-06
  • Contact: XU Li；ZHOU Rui-xiang* E-mail:zhourx@mail.fjmu.edu.cn

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摘要：

目的  探讨褪黑素(MLT)对胃癌细胞迁移和侵袭的抑制作用及其分子机制。   方法  不同浓度(0.1、1、2 和4 mmol/L)的褪黑素干预人胃癌SGC-7901 细胞 24 h，应用划痕实验、Transwell小室法检测胃癌细胞迁移和侵袭能力的改变；ELISA试剂盒检测培养液上清中基质金属蛋白酶（MMP）-2、MMP-9的表达，Real-time PCR检测胃癌细胞MMP-2、MMP-9、细胞间黏附分子1(ICAM-1）和CD44基因表达；免疫印迹法检测ICAM-1、CD44、p-P38、P38和磷酸化丝裂原活化蛋白激酶激酶（p-MKK）3/6蛋白表达。   结果  褪黑素抑制胃癌细胞的迁移和侵袭，并呈剂量依赖性。与空白对照组比较，褪黑素降低胃癌细胞基质金属蛋白酶（MMP)-2、MMP-9和ICAM-1、CD44的表达，并抑制p-P38、P38和p-MKK3/6的表达。   结论  褪黑素通过抑制胃癌细胞MMP-2、MMP-9、ICAM-1和CD44的表达从而抑制胃癌细胞的迁移和侵袭能力，抑制作用可能与p38MAPK信号通路有关。

关键词: 褪黑素, 胃癌, 基质金属蛋白酶2, 基质金属蛋白酶9, 细胞间黏附分子1, CD44, P38, 免疫印迹法

Abstract:

Objective  To investigate the inhibitory effect of melatonin on the migration and invasion of gastric cancer cell and the underlying molecular mechanism.    Methods  Human gastric cancer SGC-7901 cells were treated with different concentrations of melatonin (0.1, 1, 2 and 4 mmol/L) for 24 hours, and the changes in the migration and invasion of gastric cancer cells were detected by scratch test and Transwell assay. The expressions of matrix metalloproteinase(MMP)-2 and MMP-9 in the supernatant were detected by ELISA kit. The changes of MMP-2, MMP-9,  intercellular cell adhesion molecule-1(ICAM-1) and CD44 expressions were detected by using Real-time PCR. The protein expressions of ICAM-1, CD44, p-P38, P38 and phosphorylated mitogen-activated protein kinase kinase(p-MKK)3/6 were detected by Western blotting.    Results  Melatonin inhibited the migration and invasion of gastric cancer cells in a dose-dependent manner. Compared with the blank control group, melatonin reduced the expression of MMP-2, MMP-9, ICAM-1 and CD44, and inhibited the expressions of p-P38, P38 and p-MKK3/6 in gastric cancer cells.    Conclusion  Melatonin inhibits the migration and invasion of gastric cancer cells by inhibiting the expressions of MMP-2, MMP-9, ICAM-1 and CD44. Inhibition may be related to the p38MAPK signaling pathway. 

Key words: Melatonin, Gastric cancer, Matrix metalloproteinase-2, Matrix metalloproteinase-9, Intercellular cell adhesion molecule-1, CD44, P38, Western blotting

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