解剖学报

解剖学报 ›› 2016, Vol. 47 ›› Issue (5): 688-692.doi: 10.16098/j.issn.0529-1356.2016.05.020

• 组织学胚胎学发育生物学 • 上一篇    下一篇

神经烯醇化酶、突触素和神经纤维丝蛋白在人胚胎心脏组织中的表达

刘学红* 张泳 林敬铨 高梦丹 陈梦 焦青川   

  1. 绍兴文理学院医学院组织学与胚胎学教研室， 浙江 绍兴 312000
  • 收稿日期:2015-12-02 修回日期:2016-06-28 出版日期:2016-10-06 发布日期:2016-10-06
  • 通讯作者: 刘学红 E-mail:liuxueh6588@126.com

Expressions of neuron-specific enolase, synaptophysin and neurofilament proteins in human embryonic heart tissues

LIU Xue-hong* ZHANG Yong LIN Jing-quan GAO Meng-dan CHEN Meng JIAO Qing-chuan   

  1. Department of Histology and Embryology, School of Medicine, Shaoxing University, Zhejiang Shaoxing 312000, China
  • Received:2015-12-02 Revised:2016-06-28 Online:2016-10-06 Published:2016-10-06
  • Contact: LIU Xue-hong E-mail:liuxueh6588@126.com

摘要：

目的 探讨神经烯醇化酶(NSE)、突触素(SYN) 和神经纤维丝蛋白（NF）在人胚胎心脏组织不同发育阶段的分布特征。方法 应用免疫组织化学法，检测第2～4个月胎龄段共16份人胚胎心脏组织内NF、NSE和SYN蛋白的表达，分析其变化规律。 结果 第2～4个月龄段，NF、NSE和SYN蛋白在人胚胎心脏组织内均有阳性表达。随着胎龄的增大，NF、NSE和SYN在心脏组织内阳性表达强度值逐渐降低。第2个月龄时，NSE、NF和SYN蛋白呈少量阳性表达，阳性表达强度值分别是86.79±7.75、133.03±13.61和114.32±11.12。第3个月龄时，NSE、NF和SYN阳性表达强度值分别是81.89±9.62， 119.91±11.70和93.13±13.63。第4个月胎龄时，NSE、NF和SYN阳性表达强度值分别是72.18±11.97，107.02±10.89和91.17±13.81。应用One-Way ANOVA和 LSD-t统计学分析第2～4个月龄段人胚胎心脏组织内NSE、NF和SYN蛋白的各自阳性表达强度值，P＜0.05。 结论 第2～4个月龄段，NSE、NF和SYN均在人胚胎心肌组织内表达和呈现特定的分布规律，随胎龄增大，心肌组织内NF、NSE和SYN的表达强度逐渐增强。

关键词: 神经纤维丝蛋白, 神经烯醇化酶, 突触素, 胚胎, 心肌, 神经组织, 免疫组织化学, 人

Abstract:

Objective To explore the distribution characteristics of neuron-specific enolase（NSE）, synaptophysin（SYN）and Neurofilament protein（NF）proteins in the developmental stages of human embryonic heart tissues. Methods Immunohistochemistry was used to detect the expressions of NSE, SYN and NF proteins in the heart tissues during the second, third and fourth month of human embryonic and fetal development(16 cases). Results In the embryos at the 2nd, 3rd and 4th months of gestation, NSE, SYN and NF proteins were positively expressed in the heart tissue. With the increase of gestational age, the intensities of the positive expression of NSE, SYN and NF in the heart tissues gradually reduced. At the 2nd month of gestation, the intensities of NSE, SYN and NF protein positive expressions were 86.79±7.75，133.03±13.61 and 114.32±11.12, respectively. At the 3rd month of gestation, the intensities of NSE, SYN and NF proteins positive expressions were 81.89±9.62，119.91±11.70 and 93.13±13.63, respectively. At the 4 month of gestation, NSE, SYN and NF proteins positive expression intensity values were 72.18±11.97，107.02±10.89 and 91.17±13.81, respectively.One-Way ANOVA andLSD-t test were employed to compare positive expression intensity values of NSE, SYN and NF proteins in heart tissues detected in the 3 fetal periods, and the results of NSE, SYN and NF showed significant difference in the heart tissue(P<0.05). Conclusion At the second to fourth gestation, NSE, NF and SYN proteins show specific distribution patterns in human embryonic cardiac muscle tissues. The expression intensities of NF, NSE and SYN in myocardium increases gradually with the increase of gestational age.

Key words: Neurofilament protein, Neuron-specific enolase, Synaptophysin, Embryo, Myocardium, Nervous tissue；Immunohistochemistry, Human