脑源性神经营养因子和一氧化氮合酶在扬子鳄眼球中的定位和表达分析

doi:视网膜| 脑源性神经营养因子| 一氧化氮合酶| 免疫组织化学| 扬子鳄

解剖学报 ›› 2019, Vol. 50 ›› Issue (5): 561-564.doi: 视网膜| 脑源性神经营养因子| 一氧化氮合酶| 免疫组织化学| 扬子鳄

脑源性神经营养因子和一氧化氮合酶在扬子鳄眼球中的定位和表达分析

黄艳姣¹郑兰荣^1* 周永康²

1.皖南医学院病理解剖学教研室，安徽芜湖 241002; 2.安徽省扬子鳄繁殖研究中心，安徽宣城 242034

收稿日期:2018-11-05 修回日期:2019-01-07 出版日期:2019-10-06 发布日期:2019-12-10
通讯作者: 黄艳姣 E-mail:334129219@qq.com

Localization and expression analysis of brain-derived neurotrophic factor and nitric oxide synthase in the eyeball of Chinese alligator

HUANG Yan-jiao¹ ZHENG Lan-rong ^1* ZHOU Yong-kang²

1. Department of Pathology, Wannan Medical College, Anhui Wuhu 241002, China;2. Anhui Research Center for Chinese Alligator Reproduction，Anhui Xuancheng 242034，China

Received:2018-11-05 Revised:2019-01-07 Online:2019-10-06 Published:2019-12-10
Contact: ZHENG Lan-rong E-mail:334129219@qq.com

摘要/Abstract

摘要：

目的分析一氧化氮合酶（NOS）和脑源性神经营养因子（BDNF）在扬子鳄（Alligator sinensis）眼球的表达及分布情况。方法生物学信息分析人、家兔、非洲爪蟾和斑马鱼的BDNF和NOS氨基酸序列的同源性；HE染色观察扬子鳄眼球切片的结构；免疫组织化学染色和免疫荧光染色确定NOS及BDNF的分布部位及其位置关系。结果 BDNF的免疫阳性（绿色荧光）表达在扬子鳄眼球切片的脉络膜层和视网膜层，NOS的免疫阳性（红色荧光）则主要表达在视网膜层，两者在视网膜层有共存现象。结论 BDNF和NOS在扬子鳄的眼球中均有表达且有共存，可能参与扬子鳄视觉形成的调节。

关键词: 视网膜, 脑源性神经营养因子, 一氧化氮合酶, 免疫组织化学, 扬子鳄

Abstract:

Objective To explorethe expression and distribution of nitric oxide synthase (NOS) and brain-derived neurotrophic factor (BDNF) in the egeball of Chinese alligator,as well as the relationship between them in the eyeball. Methods The homology of amino acid sequence of BDNF or NOS proteinfromhuman, Oryctolaguscuniculus, Xenopuslaevis and Danioreriowas analyzed by bioinformatics.The distribution sites of NOS and BDNF in the alligator’s eyeball were detected using HE staining,immunohistochemistry and immunofluorescence, respectively. Results Both the choroid layer and the layer of the retina found the BDNF expressing in the alligator’s eyeball.The NOS immunopositive was abundantly expressed in the ganglion cell layer of the retina. The BDNF and NOS were both founded in the ganglion cell layer and the inner boundary layer of the retina. Conclusion BDNF and NOS are expressed in the eyeballs of Chinese alligators, which may be related to the adjustment of visual formation.

Key words: Retina, Brain-derived neurotrophic factor, Nitric oxide synthase, Immunohistochemistry, Chinese alligator

黄艳姣郑兰荣周永康. 脑源性神经营养因子和一氧化氮合酶在扬子鳄眼球中的定位和表达分析[J]. 解剖学报, 2019, 50(5): 561-564.

HUANG Yan-jiao ZHENG Lan-rong ZHOU Yong-kang. Localization and expression analysis of brain-derived neurotrophic factor and nitric oxide synthase in the eyeball of Chinese alligator[J]. Acta Anatomica Sinica, 2019, 50(5): 561-564.

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链接本文: https://jpxb.bjmu.edu.cn/CN/视网膜| 脑源性神经营养因子| 一氧化氮合酶| 免疫组织化学| 扬子鳄

https://jpxb.bjmu.edu.cn/CN/Y2019/V50/I5/561

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脑源性神经营养因子和一氧化氮合酶在扬子鳄眼球中的定位和表达分析

Localization and expression analysis of brain-derived neurotrophic factor and nitric oxide synthase in the eyeball of Chinese alligator

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