PHLDA2对肝癌细胞的增殖、侵袭、迁移和凋亡的影响

doi:10.16098/j.issn.0529-1356.2020.03.014

解剖学报 ›› 2020, Vol. 51 ›› Issue (3): 398-404.doi: 10.16098/j.issn.0529-1356.2020.03.014

PHLDA2对肝癌细胞的增殖、侵袭、迁移和凋亡的影响

马战¹ 姜钟翔²姜政^1*

1. 重庆医科大学附属第一医院消化内科，重庆 400016; 2. 重庆市人民医院呼吸内科，重庆 400013

收稿日期:2020-01-07 修回日期:2020-02-25 出版日期:2020-06-06 发布日期:2020-06-06
通讯作者: 姜政 E-mail:zhengjiang1753@163.com
基金资助:
重庆市渝中区科技计划项目

Effect of PHLDA2 on proliferation, migration, invasion and apoptosis in hepatocellular carcinoma cells

MA Zhan¹ JIANG Zhong-xiang² JIANG Zheng^1*

1. Department of Gastroenterology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;2. Department of Respiratory Medicine, Chongqing General Hospital, Congqing 400013, China

Received:2020-01-07 Revised:2020-02-25 Online:2020-06-06 Published:2020-06-06
Contact: JIANG Zheng E-mail:zhengjiang1753@163.com

摘要/Abstract

摘要：

目的探讨血小板白细胞C激酶底物同源性样结构域蛋白家族A成员2（PHLDA2）在肝癌中的表达及对肝癌细胞的增殖、侵袭、迁移和凋亡的影响。方法在线数据库基因表达谱数据动态分析（GEPIA）PHLDA2在369例肝癌组织和160例癌旁组织中的表达差异以及其表达水平对肝癌患者总体生存率的影响。包装PHLDA2敲减慢病毒。细胞计数试剂盒-8（CCK-8）和集落形成实验检测细胞增殖，Transwell法检测细胞侵袭和迁移，流式细胞术检测细胞凋亡。Western blotting检测Bax和cleaved Caspase-3蛋白的表达。结果 PHLDA2在肝癌组织中高表达，高表达的PHLDA2降低肝癌患者的总体生存率；敲低PHLDA2可以抑制肝癌细胞的增殖、侵袭和迁移能力，促进肝癌细胞凋亡。结论 PHLDA2可促进肝癌的发生发展，具有肿瘤促进因子的功能。

关键词: PHLDA2, 肝细胞癌, 增殖, 集落形成, 流式细胞术

Abstract:

Objective To observe the expression of pleckstrin homology like domain family A member 2 (PHLDA2) in hepatocellular carcinoma (HCC) and investigate the effects of PHLDA2 on cell proliferation, migration, invasion and apoptosis. Methods To analyze the expression of PHLDA2 in 369 cases of HCC tissues and 160 cases of adjacent normal tissues and the effect of PHLDA2 expression on overall survival rate of HCC patients by gene expression profiling interactive analysis (GEPIA) online database. Cell proliferation was determined by cell counting kit-8 (CCK-8) and colony formation assay. Invasion and migration were detected by Transwell assay. The percentage of apoptosis was measured by flow cytometry. The levels of Bax and cleaved Caspase-3 were measured by Western blotting. Results The expression of PHLDA2 was upregulated in HCC, and high expression of PHLDA2 reduced the overall survival of HCC patients. Low-expression of PHLDA2 inhibited proliferation, invasion and migration, and increased apoptosis of HCC cells. Conclusion PHLDA2 promotes the occurrence and development and may act as a tumor promoter in HCC.

Key words: PHLDA2, Hepatocellular carcinoma, Proliferation, Colony formation, Flow cytometry

中图分类号:

R735.7

马战姜钟翔姜政. PHLDA2对肝癌细胞的增殖、侵袭、迁移和凋亡的影响[J]. 解剖学报, 2020, 51(3): 398-404.

MA Zhan JIANG Zhong-xiang JIANG Zheng. Effect of PHLDA2 on proliferation, migration, invasion and apoptosis in hepatocellular carcinoma cells[J]. Acta Anatomica Sinica, 2020, 51(3): 398-404.

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PHLDA2对肝癌细胞的增殖、侵袭、迁移和凋亡的影响

Effect of PHLDA2 on proliferation, migration, invasion and apoptosis in hepatocellular carcinoma cells

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