解剖学报 ›› 2021, Vol. 52 ›› Issue (3): 370-376.doi: 10.16098/j.issn.0529-1356.2021.03.007

• 神经生物学 • 上一篇    下一篇

依达拉奉干预永久性脑缺血大鼠神经干细胞增殖及分化

梁晓艳1 顾玉2 赵梦1 杜鑫1 刘宏伟1 周洋1 张铁军3*   

  1. 1.承德护理职业学院药检系基础部,河北 承德 067000; 2.承德市中心医院检验科,河北 承德 067000; 3.北京市密云区中医医院骨科,北京 101500
  • 收稿日期:2019-12-26 修回日期:2020-02-10 出版日期:2021-06-06 发布日期:2021-06-06
  • 通讯作者: 张铁军 E-mail:zhenyi1174@sina.com
  • 基金资助:
    承德市科技计划基础研究项目

Edaravone intervening the proliferation and differentiation of  neural stem cells in rats with permanent cerebral ischemia

LIANG Xiao-yan1 GU Yu2 ZHAO Meng1 DU Xin1 LIU Hong-wei1 ZHOU Yang1 ZHANG Tie-jun3*   

  1. 1.Department of Basic Medicine and Drug Control, Chengde Nursing Vocational College, Hebei Chengde 067000,China; 2.Laboratory of Chengde Central Hospital, Hebei Chengde 067000,China; 3.Department of Orthopaedics, Traditional Chinese Medicine Hospital of Miyun District, Beijing 101500 China
  • Received:2019-12-26 Revised:2020-02-10 Online:2021-06-06 Published:2021-06-06
  • Contact: ZHANG Tie-jun E-mail:zhenyi1174@sina.com

摘要:

目的  探讨依达拉奉对永久性脑缺血大鼠脑内内源性神经干细胞的影响。   方法  采用电凝法建立大鼠永久性脑缺血模型。将30只SD大鼠随机分为假手术组、脑损伤组与依达拉奉组,每组10只,模型制作成功后6 h,1.5 g/L依达拉奉组腹腔注射依达拉奉(10 ml/kg),每天1次,假手术组与脑缺血组腹腔注射等体积的生理盐水,连续注射7 d。末次给药后24 h,免疫荧光染色观察损伤侧脑室下区与缺血周围脑皮质区BrdU标记阳性细胞、Nestin/BrdU标记阳性细胞、神经元Ⅲ型β-微管蛋白(Tuj1)/BrdU标记阳性细胞与胶质纤维酸性蛋白(GFAP)/BrdU标记阳性细胞,Western blotting 检测缺血周围脑皮质区Tuj1与GFAP蛋白表达。   结果  与脑缺血组比较,依达拉奉组损伤侧脑室下区与缺血周围脑皮质区的BrdU标记阳性细胞、Nestin/BrdU标记阳性细胞、Tuj1/BrdU标记阳性细胞与GFAP/BrdU标记阳性细胞明显增加(P<0.05)。与脑缺血组比较,依达拉奉组缺血周围脑皮质区的Tuj1与GFAP蛋白表达量增加(P<0.05)。   结论  依达拉奉可促进缺血损伤侧脑室下区与缺血周围脑皮质内源性神经干细胞与星形胶质细胞的增殖,促进内源性神经干细胞分化为神经元。

关键词: 脑缺血, 神经干细胞, 依达拉奉, 免疫荧光, 大鼠

Abstract:

Objective  To observe the effect of edaravone on endogenous neural stem cells in rats with permanent cerebral ischemia.    Methods  The rat model of permanent cerebral ischemia was established by electrocoagulation. Thirty SD rats were randomly divided into three groups: sham operation group, brain injury group and edaravone group. Six hours after the establishment of the model, the edaravone group was intraperitoneally injected with 1.5 g/L edaravone (10 ml/kg) once a day. The sham operation group and the cerebral ischemia group were intraperitoneally injected with saline of equal volume for 7 days. 24 hours after the last administration, BrdU positive cells, Nestin/BrdU positive cells, neuronal class Ⅲ β-tubulin(Tuj1)/BrdU positive cells and glial fibrillary acidic protein (GFAP)/BrdU positive cells were observed by immunofluorescent staining, Tuj1 and GFAP protein expressions were detected by Western blotting.    Results  Compared with the cerebral ischemia group, the BrdU positive cells, Nestin/BrdU positive cells, Tuj1/BrdU positive cells and GFAP/BrdU positive cells increased significantly in the infraventricular area and the cortex area around the ischemia in the edaravone group (P<0.05).Compared with the cerebral ischemia group, the expression of Tuj1 and GFAP protein in the cerebral cortex of edaravone group increased (P<0.05).    Conclusion  Edaravone can promote the proliferation of endogenous neural stem cells and astrocytes in the subventricular area and the cortex around ischemia, and promote the differentiation of endogenous neural stem cells into neurons.

Key words: Cerebral ischemia, Neural stem cell, Edaravone, Immunofluorescence, Rat

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