二氢杨梅素下调大鼠缺血性脑卒中脑组织炎症小体Nod样受体蛋白3的表达

doi:10.16098/j.issn.0529-1356.2022.02.001

解剖学报 ›› 2022, Vol. 53 ›› Issue (2): 137-143.doi: 10.16098/j.issn.0529-1356.2022.02.001

• 神经生物学 • 下一篇

二氢杨梅素下调大鼠缺血性脑卒中脑组织炎症小体Nod样受体蛋白3的表达

丁慧如¹邓心未¹刘怀存¹程全成¹ 方璇¹ 张国梁² 陈春花¹ 张卫光^1*

1.北京大学基础医学院人体解剖学与组织学胚胎学系，北京100083; 2.河北医科大学神经生物学研究室，石家庄 050017

收稿日期:2020-05-21 修回日期:2020-07-18 出版日期:2022-04-06 发布日期:2022-04-06
通讯作者: 张卫光 E-mail:zhangwg@bjmu.edu.cn
基金资助:
国家自然科学基金

Dihydromyricetin down-regulating the expression of nod-like receptor protein-3 in the brain tissue of ischemic stroke in rats

DING Hui-ru¹ DENG Xin-wei¹ LIU Huai-cun¹ CHENG Quan-cheng¹ FANG Xuan¹ ZHANG Guo-liang² CHEN Chun-hua¹ ZHANG Wei-guang^1*

1.Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Peking University, Beijing100083,China; 2.Department of Neurobiology, Hebei Medical University, Shijiazhuang050017, China

Received:2020-05-21 Revised:2020-07-18 Online:2022-04-06 Published:2022-04-06
Contact: ZHANG Wei-guang E-mail:zhangwg@bjmu.edu.cn

摘要/Abstract

摘要：

目的研究二氢杨梅素（DHM）在大鼠缺血性脑卒中治疗方面的作用，并探讨其对炎症小体Nod样受体蛋白3（NLRP3）表达的影响。方法取70只SD大鼠，利用线栓法构建大鼠大脑中动脉栓塞（MCAO）模型，采用Longa评分、TTC染色、Nissl染色、免疫组织化学染色及Western blotting等方法，探讨DHM对MCAO大鼠缺血/再灌注损伤的治疗效果及机制。结果 DHM治疗后MCAO大鼠自主运动能力明显改善，脑组织梗死体积明显减小，脑组织结构和神经元形态较为改善，且DHM治疗后的NLRP3和炎症因子白细胞介素1β（IL-1β）蛋白表达显著降低。结论 DHM能下调炎症小体的表达，进而降低MCAO脑卒中大鼠的脑梗死体积并改善神经行为学表现。

关键词: 缺血性脑卒中, 大脑中动脉栓塞, 二氢杨梅素, 炎症小体, Nod样受体蛋白3, TTC染色, Longa评分, 大鼠

Abstract:

Objective To investigate the role of dihydromyricetin (DHM) in the treatment of ischemic stroke in rats, and to explore the effect of DHM on the expression of inflammasome. Methods The middle cerebral artery occlusion (MCAO) model was induced by endovascular suture method. The therapeutic effect and mechanism of DHM were investigated by Longa score, TTC staining, Nissl staining, immunohistochemical staining and Western bloting. Results After DHM treatment, the motor capacity of MCAO rats was significantly improved, the infarct volume was significantly reduced, the brain structure and neuron morphology were improved, and the expressions of nod-like receptor protein-3 (NLRP3) and interleukin-1β(IL-1β) decreased significantly. Conclusion DHM can down-regulate the expression of NLRP3 and thus reduces the cerebral infarction volume and improves neurobehavioral performance in MCAO rats.

Key words: Ischemic stroke, Middle cerebral artery occlusion, Dihydromyricetin, Inflammasome, Nod-like receptor protein-3, TTC staining, Longa score, Rat

中图分类号:

R743

丁慧如邓心未刘怀存程全成方璇张国梁陈春花张卫光 . 二氢杨梅素下调大鼠缺血性脑卒中脑组织炎症小体Nod样受体蛋白3的表达[J]. 解剖学报, 2022, 53(2): 137-143.

DING Hui-ru DENG Xin-wei LIU Huai-cun CHENG Quan-cheng FANG Xuan ZHANG Guo-liang CHEN Chun-hua ZHANG Wei-guang. Dihydromyricetin down-regulating the expression of nod-like receptor protein-3 in the brain tissue of ischemic stroke in rats[J]. Acta Anatomica Sinica, 2022, 53(2): 137-143.

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吴冶君, 陈春花. 缺血性脑卒中治疗时间窗后辅助溶栓治疗的进展［J］. 解剖学报, 2019, 50(6):850-856.

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二氢杨梅素下调大鼠缺血性脑卒中脑组织炎症小体Nod样受体蛋白3的表达

Dihydromyricetin down-regulating the expression of nod-like receptor protein-3 in the brain tissue of ischemic stroke in rats

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