斑马鱼3D解剖互动系统的制作

摘要/Abstract

摘要： 目的旨在制作斑马鱼三维（three-dimension, 3D）解剖互动系统软件。方法采用光镜和Micro-CT的方法研究了斑马鱼的组织构造，并收集其形态结构图像。使用Photoshop对获得的连续切片（HE染色）的图片进行校正，使用Autodesk 3ds Max软件进行三维模型建立。结果获得了斑马鱼骨骼的3D成像和连续切片图像（雄鱼144张，雌鱼179张），实现了斑马鱼重要器官的3D重构，建立了斑马鱼3D解剖互动系统(V 1.0)，获得了计算机软件著作权登记证书（证书号：软著登字第0989966号）。结论斑马鱼3D解剖互动系统软件制作的完成为研究斑马鱼立体形态学奠定了基础，有助于研究人员掌握斑马鱼的组织形态。

关键词: 斑马鱼, Micro-CT, 连续切片, 图片校正, 三维重建

Abstract: Objective The aim of this paper was to make a software of three-dimension (3D) anatomical interaction system in zebrafish (Danio rerio). Methods The structure of zebrafish was observed by light microscopy and Micro-CT, and the images of its morphological structure were collected. The pictures of serial section (HE-stained) were corrected by Photoshop. The 3D model was established by the software of Autodesk 3ds Max. Results The results showed the zebrafish skeletal 3D imaging and serial section images (male: 144, female: 179). We realized the 3D reconstruction of the vital organs of zebrafish, and established the 3D anatomy interaction system zebrafish (V 1.0), which obtained the computer software copyright registration certificate (Certificate Number: soft word board the Di_0989966). Conclusion The zebrafish 3D anatomy interaction system software afforded the three-dimensional morphological basis for the study of zebrafish, and was helpful to histomorphology recognization for researchers.

Key words: zebrafish, Micro-CT, serial section, picture correction, three-dimension (3D) reconstruction

林金杏章琳俐胡建华高诚冯丽萍魏晓锋陈微陈秋生. 斑马鱼3D解剖互动系统的制作[J]. , 2016, 47(1): 139-144.

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