关键词: 藤梨根提取物, 食管肿瘤, 基因, 生存素, 免疫组织化学, 反转录-聚合酶链式反应, 裸鼠

Abstract: Objective To study the effect of acetidin extractive of Radix Actinidiae on transplantation tumor of esophagus cancer EC109 cells in nude mice,and to explore its mechanism of action. Methods Establish the model of transplanted tumor bearing esophagus cancer EC109 cells in nude mice and to detect the effect of acetidin extractive of Radix Actinidiae EM>in vivo/EM>. Control group and treatment groups (high-dose group and low-dose group)were set for comparison,there was six nude mice in each group．The transplanted tumor growth curve was drawn according to transplanted tumor volume, and inhibition ratio was calculated according to the final tumor weight. Terminal deoxynucleotidyl transferase mediated UTP nick end labeling (TUNEL) was used to examine the apoptosis index of the transplanted tumor.Immunohistochemistry (SP)was used to examine the expression of Survivin protein and Ki-67 antigen in the transplanted tumor.RT-PCR was used to examine the expression of Survivin mRNA. Results The growth of transplanted tumor in treatment groups became slow after taking drug.The final tumor volume and tumor weight of treatment groups were smaller than that of control group. The tumor weight inhibiting rates of highdose group and lowdose group were 64.57% and 46.78% individually. The apoptosis index of the transplanted tumor of treatment groups was higher than that of control group. The expression of Survivin mRNA and protein,Ki-67 antigen in treatment groups were lower than that of control group. And high-dose group was more differently. The statistically significant difference was found between the index of each groups that have mentioned (P<0.01).Conclusion The acetidin extractive of Radix Actinidiae has the inhibitory effect on the growth of transplanted tumor of esophagus cancer EC109 cell in nude mice.To inhibit proliferation activity of transplantation tumor,to degrade the expression of Survivin protein and mRNA,and to induce apoptosis of cancer cells might b

Key words: Radix Actinidiae extractive, Esophagus cancer, Gene, Survivin, Immunohistochemistry, RT-PCR, Nude mouse