解剖学报

解剖学报 ›› 2023, Vol. 54 ›› Issue (3): 313-318.doi: 10.16098/j.issn.0529-1356.2023.03.009

• 细胞和分子生物学 • 上一篇    下一篇

青海省汉族妊娠期高血压疾病与维生素D受体基因FokⅠ多态性的关系

武震1,2 段倩1 袁明1 王雪妮1 陈文静1 李玉琴3 俄洛吉3 李建华1*
  

  1. 1. 青海大学医学院基础医学部人体解剖学教研室，西宁810016; 2. 河南大学第一附属医院超声科，河南 开封 475000; 3. 青海省人民医院产科，西宁 810007
  • 收稿日期:2022-04-13 修回日期:2022-06-02 出版日期:2023-06-06 发布日期:2023-06-06
  • 通讯作者: 李建华 E-mail:1184666039@qq.com
  • 基金资助:
    青藏高原人类遗传资源样本库建设;青海地区藏族与汉族产妇血清指标PLGF及VEGF、MTHFR、AGT基因多态性与子痫前期的相关性研究

Relationship between FokⅠ polymorphism of vitamin-D receptor gene and hypertensive disorder complicating pregnancy in Han nationality of Qinghai province

WU  Zhen1, 2  DUAN  Qian1  YUAN  Ming1  WANG  Xue-ni1  CHEN  Wen-jing1  LI  Yu-qin3  E-Luo-Ji3  LI  Jian-hua1*   

  1. 1.Department of Human Anatomy，Basic Medicine Science，Medical College of Qinghai University, Xining810016,China;  2.Department of Ultrasound Medicine，the First Affiliated Hospital of Henan University，He’nan, Kaifeng 475000,China;  3.Department of Obstetrics，Qinghai Provincial People’s Hospital, Xining810007,China
  • Received:2022-04-13 Revised:2022-06-02 Online:2023-06-06 Published:2023-06-06
  • Contact: LI Jian-hua E-mail:1184666039@qq.com

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摘要：

目的 探讨维生素D受体（VDR）基因单核苷酸多态性（SNP）FokⅠ（rs2228570/rs10735810）位点多态性与青海省汉族妇女妊娠期高血压疾病（HDCP）的相关性。  方法 选择青海省汉族HDCP患者137例（HDCP组），正常汉族孕妇146例（对照组），使用聚合酶链式反应-限制性核酸内切酶片段长度多态性（PCR-RFLP）方法检测HDCP组和对照组FokⅠ位点的基因分型并测序验证，使用SPSS 19.0统计学软件检验两组一般临床资料、基因型及等位基因频率分布差异是否具有统计学意义。  结果 HDCP组和对照组FokⅠ位点FF、Ff、ff基因型频率分别为51.82%、37.96%、10.22%和34.93%、43.15%和21.92%（χ2=11.099，P<0.05），HDCP组FF基因型（OR=2.004，95% CI=1.243~3.231）频率高于对照组；HDCP组和对照组FokⅠ位点F和f等位基因频率分布差异有统计学意义（χ2=12.454，P<0.001），HDCP组F等位基因（OR=1.253，95% CI=1.105~1.421）频率高于对照组。  结论 VDR基因FokⅠ位点多态性与青海省汉族HDCP相关，其中F等位基因可能是HDCP的易感基因，FF基因型可能是HDCP的易感基因型。

关键词: 青海省, 妊娠期高血压疾病, 维生素D受体, 基因多态性, FokⅠ位点, 聚合酶链式反应-限制性核酸内切酶片段长度多态性, 孕妇

Abstract:

Objective To investigate the relationship between single nucleotide polymorphism (SNP) FokⅠ（rs2228570/rs10735810）of vitamin D receptor (VDR) gene and hypertensive disorder complicating pregnancy (HDCP) in Han nationality women of Qinghai province.   Methods A total of 137 Han nationality HDCP subjects (HDCP group) and 146 Han nationality normal pregnant subjects (control group) were selected from Qinghai province. The FokⅠ polymorphism typing in HCDP group and control group was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The mutation was confirmed by sequencing. SPSS 19.0 statistical software was used to test whether there were significant differences between two groups in general clinical data, genotype and allele frequency distribution.   Results The frequency of FF Ff ff genotype of FokⅠ in HDCP group and control group were 51.82%, 37.96%, 10.22% and 34.93%, 43.15%, 21.92% respectively (χ2=11.099, P<0.05, the distribution of genotype was different significantly between the two groups), the frequency of FF genotype in HDCP group (OR=2.004, 95%CI=1.243-3.231) was higher than that in control group; The frequency distribution of alleles F and f of FokⅠ was significantly different between HDCP group and control group (χ2=12.454, P<0.001), F allele frequency in HDCP group (OR=1.253, 95%CI=1.105-1.421) was higher than that in control group.   Conclusion The FokⅠ polymorphism of VDR gene is significantly correlated with hypertensive disorder complicating pregnancy of Han nationality in Qinghai. The F allele may be the susceptibility gene of HDCP, the FF genotype may be the susceptibility genotype of HDCP.

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