Acta Anatomica Sinica

AAS ›› 2015, Vol. 46 ›› Issue (1): 94-100.doi: 10.16098/j.issn.0529-1356.2015.01.017

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Influence of 5-hydroxytryptamine on stress-induced diarrhea of weaning mice

YANG Chen-yu1 HAN Ya-nan1 WANG Zi-xu1 CHEN Yao-xing1 QIN Zhuo-ming2 CAO Jing1 SONG Jin-yuan1 DONG Yu-lan 1*   

  1. 1. College of Veterinary Medicine, China Agricultural University, Beijing 100193，China; 2. Institute of Poultry, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China
  • Received:2014-05-20 Revised:2014-08-25 Online:2015-02-06 Published:2015-02-06
  • Contact: DONG Yu-lan E-mail:ylbcdong@cau.edu.cn

Abstract:

Objective To investigate the relationship between stress-induced diarrhea and 5-hydroxytryptamine (5-HT) and explore the possible mechanism of stress-induced diarrhea. Methods Citalopram hydrobromide (CH) was used to increase 5-HT content and para-chlorophenylalanine (PCPA) was used to decrease 5-HT intracellular synthesis. Seventy-two male 21 days newly weaned ICR mice were randomly divided into six groups: control, CH group, PCPA group, stress diarrhea group, CH+stress diarrhea group, PCPA+stress diarrhea group. Daily administration of intraperitoneal injection of CH 10 mg/kg or PCPA 300mg/kg, 4 hours later, the last three groups were administrated with intragastric of senna (0.4 kg/L) with 15ml/kg BW dose and hind legs binding stress for 4 hours. The control group was administrated with equal relative doses of saline. Five days later detected blood sugar levels of animals were detected, ELISA and immunohistochemical staining was used to measure 5-HT and corticosterone (Cort) content. Results The data of stress diarrhea and CH treated mice showed an increase in diarrhea score, blood sugar levels and Cort in plasma, but a significant decrease in weight gain coincided with an increase in 5-HT in plasma and intestine compared to that of control animals. Stress diarrhea mice following CH administration showed significantly raised 5-HT in plasma and intestine, which was in coincided with the increased diarrhea score but the greatly decreased weight gain of animals when compared to stress diarrhea mice. Administration of PCPA to mice did not change significantly weight gain and blood sugar levels, but increased Cort content compared to control animals. While treatment with PCPA to stress diarrhea mice resulted in a significantly increase in weight gain and decrease in blood sugar levels, 5-HT, and Cort content compared to stress diarrhea mice, but still was higher than that of control animals. Conclusion 5-HT content in stress diarrhea mice significantly increased, in turn, CH-induced increase of 5-HT content directly resulted in and aggravated stress-induced diarrhea of weaning mice. Conversely, reducing 5-HT content induced by PCPA weakened degree of diarrhea.

Key words: Stress diarrhea, 5-Hydroxytryptamine, Citalopram hydrobromide, Para-chlorophenylalanine, Immunohistochemistry, Newly weaned mouse