Effect of oxidative damage on malignant transformation of bone marrow-derived hepatic progenitor cells

doi:10.16098/j.issn.0529-1356.2015.06.009

Abstract

Abstract:

Objective To explore the role of oxidative damage in malignant transformation of bone marrow-derived liver stem cells. Methods Bone marrow mesenchymal stem cells (BMSCs) were divided into three groups: control group（BMSCs）, GF induction group（BMSCs+GF）and Hydrogen peroxide stimulate group（BMSCs+GF+ H₂O₂). Albumin (ALB) mRNA, alpha-fetoprotein (AFP) mRNA and CK18 mRNA were detected by RT-PCR at 7d, 14d, 21d and 28d. Gamma glutamyl transpeptidase (γ-GT) was detected by immunocytochemical technique. DNA contents were detected by Schiff dyeing. Aneuploid somatic cells were detected by flow cytometry. Results The results of in vitro experiments showed that there were expressions of ALB mRNA, AFP mRNA and CK18mRNA in group BMSCs+GF and group BMSCs+GF+ H₂O₂. The expressions of AFP mRNA, CK18mRNA, γ-GT, DNA contents and aneuploid somatic cells in group BMSCs+GF+ H₂O₂ were higher than that in group BMSCs+GF. Conclusion Reactive oxygen species can induce bone marrow mesenchymal stem cell proliferation and malignant transformation.

Key words: Bone marrow mesenchymal stem cell, Oxidative damage, Malignant transformation, Liver cancer, Microenvironment, Reverse transcription-polymerase chain reaction, Rat

WEN Zhao-yang SHI Zheng-ming ZHAO Na XUE Xiao-wei FENG Ping WANG Xue-jiang*. Effect of oxidative damage on malignant transformation of bone marrow-derived hepatic progenitor cells[J]. AAS, 2015, 46(6): 780-784.

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