Change of learning and memory ability in neonatal rats with hypoxic-ischemic white matter injury

doi:10.16098/j.issn.0529-1356.2016.06.003

Abstract

Abstract:

Objective To explore changes of the learning and memory ability as well as the myelin basic protein (MBP) expression during various growth periods in neonatal rats with hypoxic-ischemic white matter injury (WMI). Methods New born 4-day-old SD rats were randomly divided into a control group and a hypoxicischemic WMI group. The histology of rat brain tissues was observed by HE staining at 4w after operation. The neurobehavior and the ability of learning and memory were investigated at 4weeks, 8weeks and 12weeks after operation. The distribution and expression of MBP protein were tested by immunofluorescent histochemical staining method and western blotting. Results The hypoxic-ischemic white matter injury was showed by HE staining in WMI group. The weaker ability of neurobehavior and learning and memory were displayed in WMI group compared with the control group (P＜0.01). The expression of MBP was significantly decreased in the WMI group compared with the control group（P＜0.01）. Conclusion The ability of learning and memory was weakened and the expression of MBP protein was decreased in new born 4-day-old SD rats with hypoxic-ischemic white matter injury. The weaker ability of learning and memory may be resulted from the decreased expression of MBP protein.

Key words: White matter injury, Learning, Memory, Myelin basic protein, Immunofluorescence, Western blotting, Rat

LIN Ling ZHANG Geng LIN Qiao-mei LIN Qing. Change of learning and memory ability in neonatal rats with hypoxic-ischemic white matter injury[J]. Acta Anatomica Sinica, 2016, 47(6): 738-743.

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