Intermittent starvation attenuating neurofilament phosphorylation and spatial learning and memory disorder induced by sodium nitrite in rats

doi:10.16098/j.issn.0529-1356.2017.01.003

Abstract

Abstract:

Objective To explore the effect of intermittent starvation on neurofilament phosphorylation and spatial learning and memory in rats induced by sodium nitrite.Methods Thirty-six rats were randomly divided into control, NaNO₂ and stavation+NaNO₂ groups, 12 rats in each group. NaNO₂group and stavation+NaNO₂ group rats were served as drinking water containing sodium nitrite ［100mg/(kg·d)］. Starvation+NaNO₂ group rats were deprived of food for 2 days, and then re-feeded for 3 days. Sixty days later, the ability of spatial learning and memory of the rats was measured by Morris water maze. Phosphorylation level of neurofilament in the hippocampus was detected by immunohistochemistry and Western blotting. Results In comparison with the control rats, the NaNO₂ rats showed significantly longer latency to find the hidden platform from 2 days to 7 days in Morris water maze (control group latency: 31.24±8.53，12.41±6.54，10.49±6.43，8.61±2.56，7.25±2.12，6.03±1.92; NaNO₂ group latency: 53.34±5.28，35.15±10.29，23.52±9.50，14.49±8.70，16.87±8.77，12.31±7.12) (P<0.05,P<0.01), and elevated phosphorylation level of neurofilament, while stavation+NaNO₂ group rats only showed longer latency on 2 days and 3 days（43.61±1.76，25.25±7.49）in Morris water maze. There was no obvious difference in phosphorylation level of neurofilament between the control and stavation+NaNO₂ group. Conclusion Intermittent starvation attenuates neurofilament phosphorylation and spatial learning and memory disorder induced by sodium nitrite in rats.

Key words: Intermittent starvation, Sodium nitrite, Neurofilament, Phosphorylation, Learning, Memory, Western blotting, Rat

HU Zhi-hong YAN Jun-bao LI San-qiang HU Yong-mei. Intermittent starvation attenuating neurofilament phosphorylation and spatial learning and memory disorder induced by sodium nitrite in rats[J]. Acta Anatomica Sinica, 2017, 48(1): 14-18.

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