Heat shock protein 70 and Hantavirus persistent infection of cortical astrocytes from newborn rats

doi:10.16098/j.issn.0529-1356.2018.01.002

Abstract

Abstract:

Objective To define that heat shock protein 70（HSP70）overpression might contributed to the persistent infection of Hantaviruses （HTNV）. Methods The expression of HSP70 in cortical astrocytes was examined by Western blotting and RT-PCR during Hantaviruses persistent infection. The following apoptosis was detected by immunofluorescence microscopy, AnnexinV assays and RT-PCR assay. Results Compared to control group ,the expression of HSP70 protein in astrocytes infected with HTNV 76-118 or SEOV L99 increased, peak expression of HSP70 at day 3 following HTNV 76-118 infection or at day 2 following SEOV infection (n=15, P<0.01, P<0.05), and the expression of HSP70 gene in astrocytes infected with HTNV 76-118 or SEOV L99 up-regulated, peak expression of HSP70 at day 2-5 post HTNV76-118 infection and at day 2-4 post SEOV L99 infection (n=15, P<0.01, P<0.05). In the course of HTNV76-118 or SEOV L99 infection, any cytopathic effect was not shown in astrocytes and cell apoptosis was not induced by viral replication. Conclusion HSP70 induced by Hantaviruses infection might inhibit apoptosis and contribute to the viral persistent infection.

Key words: Hantavirus, Cerebral cortex, Astrocyte, Heat shock protein 70, Western blotting, RT-PCR, Rat

CLC Number:

Ｒ373. 3 ⁺ 2

HE Shuai YANG Shou-jing. Heat shock protein 70 and Hantavirus persistent infection of cortical astrocytes from newborn rats[J]. Acta Anatomica Sinica, 2018, 49(1): 7-13.

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