Inhibitory effect of soybean isoflavone on benign prostatic hyperplasia and its mechanism

doi:10.16098/j.issn.0529-1356.2018.02.008

Abstract

Abstract:

Objective To observe the effects of soy isoflavones (SI) on sex hormones, growth factors and apoptosis related genes in benign prostatic hyperplasia (BPH) rats, and to explore the prevention and mechanism of SI on BPH. Methods One hundred healthy adult male SD rats (SPF) were randomly divided into the normal control (NC) group (n=20), BPH model group (n=20), low-dose of SI group (n=20), medium-dose of SI group (n=20) and high-dose of SI group (n=20). After intragastric administration for 4 weeks, the rats were anesthetized and the abdominal aorta blood collected for preparing serum. The prostate tissues were obtained from the rats after blood collection and weighed, and the prostatic indexes (PI) were calculated. Enzyme linked immunosorbent assay (ELISA) was performed to detect the levels of estradiol (E2) and testosterone (T) in serum of the rats. The expressions of Fas, FasL, Bax, Bcl-2, epidermal growth factor (EGF) and EGF receptor (EGFR) were detected by Western blotting. Results Compared with the NC group, the wet weight of prostate and PI of rats in the BPH group increased obviously (P＜0.05), the levels of E2 and T in serum enhanced significantly (P＜0.05), the expressions of FasL, Bcl-2, EGF and EGFR in the prostate tissues up-regulated evidently (P＜0.05), while the expressions of Fas and Bax in the prostate tissues down-regulated evidently (P＜0.05). Compared with the BPH group, the wet weight of prostate and PI of rats in the medium-dose of SI and high-dose of SI groups decreased obviously (P＜0.05), the levels of E2 and T in serum decreased significantly (P＜0.05), the expressions of FasL, Bcl-2, EGF and EGFR in the prostate tissues down-regulated evidently (P＜0.05), while the expressions of Fas and Bax in the prostate tissues up-regulated evidently (P＜0.05). In addition, the changes of all the above mentioned indexes in the medium-dose of SI group showed more significant than that in the low dose of SI and high dose of SI groups (P＜0.05). Conclusion SI has an inhibitory effect on BPH, especially with the medium dose. The mechanism may be related to regulating the level of sex hormones, the expression of growth factor and its receptors, as well as the expression of apoptosis genes.

Key words: Benign prostatic hyperplasia, Soybean isoflavone, Sex hormone, Euzymelinked immunosorbent assay, Western blotting, Rat

XU Guang-chi LIU Tao DONG Bo MENG Yin. Inhibitory effect of soybean isoflavone on benign prostatic hyperplasia and its mechanism[J]. Acta Anatomica Sinica, 2018, 49(2): 185-190.

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