Expression of peroxiredoxin I-thioredoxin, superoxide dismutases and catalases mRNA in hepatic ischemia-reperfusion injury of rats

doi:10.3969/j.issn.0529-1356.2013.04.020

Abstract

Abstract:

Objective To observe the expression change of peroxiredoxin I-thioredoxin (PrxI-Trx), superoxide dismutases (SOD) and catalases (CAT) in hepatic Ischemia-Reperfusion injury of rats, and to explore their role in oxidative-stress response.
Methods The hepatic ischemia-reperfusion injury model of rats was established by clipping the hepatic blood vessel released both hepatic left and middle lobes as well as bile duct pedicle with non-damage vascular clamp for 30 minutes. The serum and
hepatic tissues were taken 6 hours after clipping. The morphological changes were observed under a microscope using HE staining.The serum alanine aminotransferase(ALT) was detected by Automatic biochemical analyzer. The PrxI-Trx, SOD and CAT mRNA expression
were evaluated by RT-PCR. The protein level of PrxI, SOD and CAT were estimated by Western blotting. Results Compared with the control group, the ALT level and HE results of hepatic ischemia-reperfusion injury group showed that liver cells were significantly
impaired. The PrxI-Trx, CAT and SOD mRNA expression were increased significantly. The protein level of PrxI, SOD and CAT also increased significantly. Conclusion PrxI-Trx, SOD and CAT may play an important role in inhibiting the oxidative-stress response
induced by ischemia-reperfusion injury.

Key words: Ischemia-reperfusion injury, Peroxiredoxin I, Catalases, Superoxide dismutases, Oxidative stress, Western blotting, Rat

ZHANG Li-hua WANG Qie LIU Zhao WANG Lei*. Expression of peroxiredoxin I-thioredoxin, superoxide dismutases and catalases mRNA in hepatic ischemia-reperfusion injury of rats[J]. AAS, 2013, 44(4 ): 550-553.

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