Prenatal alcohol exposure inducing insulin resistance and stress injury in mouse hippocampi

doi:10.3969/j.issn.0529-1356.2014.01.002

Abstract

Abstract:

Objective To investigate the relationship of prenatal alcohol exposure inducing insulin resistance and the stress injury in the mouse hippocampi.
Methods C57BL/6J mice were used to establish the models of prenatal alcohol exposure with control group, moderate ethanol group and high ethanol group. The pups of different groups at postnatal day 7(P7), P14 and P30 were gathered for the measurements of fasting blood glucose and blood insulin. The insulin resistance index (HOM-IR) was calculated. The stress injury in hippocampal CA1 area was observed. For instance, the expression of stress injury proteins, c-Fos and NF-κB, of hippocampus at P7 and P14 was tested with immunofluorescent labeling and Western blotting to analysis. Results After prenatal alcohol exposure, fasting glucose and insulin resistance index increased with dose dependency and long term effect (P<0.05,n=90). After prenatal alcohol exposure, c-Fos and NF-κB positive cells increased in hippocampi with dose dependency and long term effect (P<0.05,n=90). The expression of c-Fos and NF-κB proteins with Western blotting in hippocampus was harmonized with the results of immunocytochemistry (P<0.05,n=30). Conclusion The prenatal alcohol exposure can induce pups insulin resistance, and the insulin resistance is probably caused by stress injury. In addition, the insulin resistance may be involved in the pathogenesis and brain injuries of fetal alcohol syndrome, through c-Fos and NF-κB pathway.

Key words:

Prenatal ethanol exposure, Insulin resistance, Oxidative stress, Hippocampus, Stress injury, Immunofluorescence, Western blotting, Mouse

ZHOU Shu-fang GAO Lin HE Wei-ya Cui Zhan-jjun DENG Jin-bo*. Prenatal alcohol exposure inducing insulin resistance and stress injury in mouse hippocampi[J]. AAS, 2014, 45(1): 7-14.

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