Expression of fibroblast growth factor-2 and fibroblast growth factor receptor-4 in human papillary thyroid carcinoma and their significances

doi:10.3969/j.issn.0529-1356.2014.05.016

Abstract

Abstract:

Objective To investigate the expression of fibroblast growth factor-2 (FGF-2) and fibroblast growth factor receptor-4(FGFR-4) in the papillary thyroid carcinomas（PTC）and clinical significance. Methods Immunohistochemistry and Western blotting for the expression of FGF-2 and FGFR-4 were performed in 89 cases of PTC and 30 cases of normal thyroid tissues (NTT) adjacent to the tumors. Results Immunohistochemistry results showed that, FGF-2 and FGFR-4 expressions were high in thyroid carcinoma (P<0.01，P<0.01)in contrast to that in the normal thyroid tissues, and the difference was statistically significant;There was a positive linear correlation between expressions of FGF-2 and FGFR-4 and lymph node metastasis (χ²=14.798,P<0.01;χ²=7.27,P<0.01)and differentiation degree (χ²=13.824,P<0.01;χ²=16.921, P<0.01) in papillary thyroid carcinoma,while there was no difference in gender,age and tumor size(P>0.05). Analyzed by Western blotting technique,FGF-2 and FGFR-4 expressions in thyroid carcinoma were significantly higher than that in normal tissue,with decrease of cancer degree of tissue differentiation and significantly up regulated expression (P< 0.05). Expressions of FGF-2 and FGFR-4 were in a positive linear correlation in the disease（rs=0.434,P<0.01）. Conclusion The expressions of FGF-2 and FGFR-4 are correlated with papillary thyroid cancer and they participated in the process of invasion and metastasis, both of which have a positive synergistic effect. The degree of malignancy and biological behavior are meaningful and comprehensive indicators,which provide a theoretical basis for the subsequent experimental studies of cellular and molecular biology.

Key words: Fibroblast growth factor-2, Fibroblast growth factor receptor-4, Invasion, Metastasis, Papillary thyroid carcinoma, Western blotting, Human

LI Qian LI Li GUO Shu-qin* ZHANG Yun-liang KONG Fan-qiang LI Fei KANG Jun-peng WU Jing-fang GAO Fu-lu. Expression of fibroblast growth factor-2 and fibroblast growth factor receptor-4 in human papillary thyroid carcinoma and their significances[J]. AAS, 2014, 45(5): 675-681.

References

［1］Hundahl SA，Fleming ID，FremgenAM，et al. A National Cancer Data Base report on 53 856 cases of thyroid carcinoma treated in the U. S，1985-1995［J］. Cancer，1998，83(12)：2638-2648. 
［2］Luo ShL,Yu M, Gong WW.The popularity of thyroid status and preventive factors［J］. China Preventiv Medicine,2013,14(4):317. (in Chinese)
罗胜兰，俞敏，龚巍巍.甲状腺癌的流行现况及其危险因素［J］.中国预防医学杂志，2013,14 (4):317.
［3］Xiao DSh,Cao HQ,Deng ZhH,et al.Effect of FGF-2 and subcellur location of Smac in human small cell lung cancer cell NCIH446［J］.Journal of Central South University (Medical Science),2008,33(8)：705-711. (in Chinese)
肖德胜,曹慧秋,邓征浩，等.FGF-2对小细胞肺癌细胞株NCIH446 survivin表达及Smac亚细胞定位的影响［J］.中南大学学报（医学版）,2008,33(8)：705-711.
［4］Liu W,Deng ZhH,Zhou JH,et al.Clinicopathological significance of FGF-2 and TRX expression in non-small cell lung cancer［J］.China Journal of Modern Medicine,2010,20(5):1461-1465. (in Chinese)
刘玮,邓征浩,周建华，等.FGF-2和TRX在非小细胞肺癌中的表达及临床病理学意义［J］.中国现代医学杂志,2010,20(5):1461-1465.
［5］Yao R,Sun J,Li ZhY,et al.Fibroblast growth factor 2 regulation of bone sialoprotein expression in human breast cancer cells［J］.Journal of Logistics University of CAPF(Medical Sciences ),2013,22(5):348-350. (in Chinese)
姚睿,孙静,李正阳, 等.FGF-2对人乳腺癌细胞MCF7骨涎蛋白表达的影响［J］.武警后勤学院学报(医学版）， 2013, 22(5): 348-350.
［6］Gowardhan B,Douglas DA,Mathers ME,et al.Evaluation of the fibroblast growth factor system as a potential target for therapy in human prostate cancer［J］.Br J Cancer,2005,92(2):320-327.
［7］Wang J,Stockton DW, Ittmann M.The fibroblast growth factor receptor-4 Arg388 allele is associated with prostate cancer initiation and progression［J］.Clin Cancer Res,2004,10(18):6169-6178.
［8］Thussbas C,Nahrig J,Streit S,et al.FGFR4 Arg388 allele is associated with resistance to adjuvant therapy in primary breast cancer［J］.J Clin Oncol,2006,24(23):3747-3755.
［9］Zhu CP,Yi ZhW.The expression of FGFR4 in the development of kidney and renal pathology ［J］.Contemporary Chinese Journal Pediatrics, 2007,9(2):133-138. (in Chinese)
朱翠平,易著文.成纤维细胞生长因子受体4在人发育肾和病理肾中的表达［J］.中国当代儿科杂志，2007,9(2):133-138.
［10］Shereen E,Ping H,Alan D,et al.Dual inhibition of RET and FGFR4 restrains medullary thyroid cancer cell growth ［J］.Clin Cancer Res,2005,11(3):1336-1341.
［11］Rose K.Foscarnet reduces FGF2-induced proliferation of human umbilical vein endothelial cells and has antineoplastic activity against human anaplastic thyroid carcinoma cells［J］.Biomed Pharmacother,2013, 67(1):53-57.
［12］St Bernard R,Zhang L, Liu W, et al. Fibroblast growth factor receptors as molecular targets in thyroid carcinoma［J］.Endocrinology,2005,146(3):1145-1153.

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