Expression of the autophagy-related protein in hippocampus in a rat model of autism

doi:10.3969/j.issn.0529-1356.2014.06.007

Abstract

Abstract:

Objective To investigate the effect of autophagy in the rat model of autism. Methods Twenty female Wistar rats (weight 250-260g) were mated with 10 male (weight 280-290g) overnight, and in the morning when spermatozoa were found it was designated as the first day of gestation. Females received a single intraperitoneal injection of sodium valproate on the 12.5 day after conception, and control females were injected with physiological saline at the same time. The offspring of valproate-treated females were model group, and the offspring of physiological saline -treated females were control group. The self-grooming test and three-chambered social test were used to confirm whether the autism model were successful. Western blotting was used to detect the expressed level of LC3-Ⅱ, Beclin 1 and P62 in hippocampal of eatch group. Results 1.The animal model of autism was successfully established. Compared with the control group, the cumulative self-grooming time was significantly increased（P<0.05）, the sociability and preference for social novelty were significantly decreased（P<0.05）. 2. Compared with normal control group, the model group significantly decreased the expression level of LC3-Ⅱ（P＜0.05）and Beclin 1（P＜0.05）in hippocampal. Meanwhile the expression level of P62 was significantly increased（P＜0.05）. Conclusion The present findings confirmed that the autophagy is inhibitied in hippocampus of autism, which suggests modulation of autophagy might be a potential therapeutic strategy for autism.

Key words: Autism, Hippocampus, Programmed cell death, Autophagy, Western blotting, Rat

CHEN Yun-hua LI Hong-wei TONG Xue-tao WEN Min*. Expression of the autophagy-related protein in hippocampus in a rat model of autism[J]. AAS, 2014, 45(6): 768-772.

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