Expressions of the RECK, matrix metalloproteinases-9 and vascular endothelial/growth factor in placentas of preeclamptic patients

doi:10.3969/j.issn.0529-1356.2014.06.017

Abstract

Abstract:

Objective To investigate the expression of reversion-inducing cysteine-rich protein with reversion-inducing-cysteine-rich protein with kazal motifs (RECK), matrix metalloproteinase-9 (MMP-9) as well as vascular endothelial growth factor (VEGF) genes in placenta of preeclamptic patients, and discuss the regulation of these genes in invasion of placenta trophoblasts. Methods Reverse transcription polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry were employed in the detection of expression of RECK gene, MMP-9 and VEGF in placenta tissues from 120 gravidae with full-term pregnancy undergoing cesarean section, i.e., normal pregnancy group (n=30), mild preeclampsia group (n=30), moderate preeclampsia group (n=30) and severe preeclampsia group (n=30). Results The mRNA expression level of MMP-9 and VEGF in three preeclampsia groups were all significantly lower (P<0.05) than those in normal pregnancy group. The expression level of RECK mRNA in the severe preeclampsia group was significantly higher (P<0.05) than that in normal pregnancy group. The expression of MMP-9 and VEGF proteins in three preeclampsia groups were lower than those in normal pregnancy group with statistically significant differences (P<0.05)，and the expression of RECK protein in moderate and severe preeclampsia groups were both significantly higher (P<0.05) than that in normal pregnancy group. Conclusion The expression level of RECK is negatively correlated with those of MMP-2 and MMP-9 in preeclamptic patients, which indicates that they are involved in regulating the process of shallow invasion of trophoblasts in placenta.

Key words: RECK gene, Matrix metalloproteinase-9, Vascular endothelial growth factor, Preeclampsia, Placenta, Western blotting, Immunohistochemistry, Human

HUA Mao-fang * LIU Xiao-yun LI SHan-feng . Expressions of the RECK, matrix metalloproteinases-9 and vascular endothelial/growth factor in placentas of preeclamptic patients[J]. AAS, 2014, 45(6): 830-834.

References

［1］Cao ZY, Shen Q, Duan T, et al. Obstetrics and Gynecology［M］. Beijing: People’s Medical Publishing House, 2010：144. (in Chinese)
    曹泽毅, 沈铿, 段涛,等. 妇产科学［M］.北京:人民卫生出版社,2010：144.
［2］Maynard S,Epsteinf H,Karumanchis A.Preecl-ampsia and angiogenic imbalance［J］. Annu Rev Med,2008,59(1):6l-78.
［3］Tao J, Fan JX. Extravillous trophoblast in spiral arterial remodeling and pre-eclampsla［J］.Journal of International Obstetrics and Gynecology，2011,38(3):174-176. (in Chinese)
    陶俊,范建霞. 绒毛外滋养细胞与螺旋动脉重铸及子痫前期［J］. 国际妇产科学杂志，2011,38(3):174-176.
［4］Li Q, Qiu Sh, Wang JJ. The expression and significance of VEGF in the preeclampsia placenta［J］. Hebei Medicine, 2010，10(16)：1157-1159. (in Chinese)
    李芹, 仇姝，王建军. VEGF蛋白在子痫前期胎盘中的表达及意义［J］.河北医学, 2010，10(16)：1157-1159.
［5］Xie X, Gou WL. Obstetrics and Gynecology［M］.8th ed.Beijing: People’s Medical Publishing House, 2013:64.(in Chinese)
    谢幸,苟文丽主编. 妇产科学［M］. 第8版.北京:人民卫生出版社,2013:64.
［6］Figueira RC,Comes LR,Neto JS,et al.Correlation between MMPs and their inhibitors in breast cancer tumor tissue specimens and in cell lines wit different metastatic potential［J］.BMC Cancer,2009,9:20.
［7］Takagi S, Simizu S, Osada H. RECK negatively regulates matrix metalloproteinase transcription［J］. Cancer Res, 2009,69(4):1502-1508.
［8］Guo JH，Xu L, Zhang WM, et al. Expression of RECK gene in placentas from patients with preeclampsia and its correlation with MMP-2 activation［J］. Acta Medicinae Universitatis Scientiae et Technologiae Huazhong, 2009,38（6）:818-820. (in Chinese)
    郭君红, 徐亮, 张文淼, 等. RECK基因在子痫前期患者胎盘的表达及其与MMP2活化的关系［J］.华中科技大学学报（医学版）,2009,38（6）:818-820.
［9］Fang YY, Liu FM, Zhu XW, et al. Expression of matrix metalloproteinase suppressor gene RECK in villi of early spontaneous abortion and its significance［J］. Acta Academiae Medicinae Xuzhou,,2010,30(5):336-339. (in Chinese)
    方媛媛,刘福民,朱学文,等. 基质金属蛋白酶抑制基因RECK在早孕自然流产绒毛组织中的表达及意义［J］.徐州医学院学报,2010,30(5):336-339.
［10］Liang MQ, Pang LH, Shi L, et al. Expression and significance of matrix luetalloproteinase-9 and vascular endothelial growth factor in hypertensive disorder in pregnancy［J］. Journal of Minimally lnvasive Medicine, 2010,5（4）:338-339.(in Chinese)
    梁美秋,庞丽红, 石凌, 等.基质金属蛋白酶9和血管内皮生长因子在妊娠高血压疾病中的表达及其意义［J］. 微创医学,2010,5（4）:338-339.
［11］Peters TJ, Albieri A, Bevilacqua E, et al. Differention-dependent expression of gelatinase B/matrix metalloproteinase-9 in trophoblast cells［J］. Cell Tissue Res, 1999, 295(2):287-296.
［12］Liu X, Li L. Expression and role of placental vascular endothelial growth factor in patients with pregnancy induced hypertension［J］. Chinese Journal of Obstetrics and Gynecology,2002,37（1）:22-24. (in Chinese)
    刘霞,李力.妊娠高血压患者胎盘血管内皮生长因子的表达及其意义［J］.中华妇产科杂志,2002,37（1）:22-24.

[1]	HONG Xiao-min LI San-qiang CUI Qin-yi ZHENG Run-yue YANG Meng-li LUO Ren-li LI Qian-hui. Nuclear factor-κB signaling pathway and gender differences in alcoholic liver fibrosis [J]. Acta Anatomica Sinica, 2024, 55(1): 55-61.
[2]	WEI Xi-xi LI Chao-hong ZHAO Chen-lu TANG Jia-ping LIU Yu-zhen. Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia [J]. Acta Anatomica Sinica, 2024, 55(1): 3-9.
[3]	LIU Xiao-li XU Lin WU Ai-xue WEN Cai-yun LI Ru-hua WU An-ting CHEN Dai-qian CHEN Cheng-chun. Analysis of cerebral gray matter structure in multiple sclerosis and neuromyelitis optica [J]. Acta Anatomica Sinica, 2024, 55(1): 17-24.
[4]	YUAN Lei YANG Zhi-wei YANG Hui LIU Zheng-hai HE Jie WAN Wei. Panax notoginseng saponin relieving the inflammatory pain caused by complete Freund’s adjuvant by inhibiting the activation of astrocytes in mice [J]. Acta Anatomica Sinica, 2024, 55(1): 25-31.
[5]	MA Ting-ting CHEN Jian-hong LIU Ai-cui LI Hai-ning. Role of inhibiting lncRNA TUG1 to down-regulate nucleotide binding oligomerization domain like receptor protein 1 inflammasome in delaying the progression of Alzheimer’s disease [J]. Acta Anatomica Sinica, 2024, 55(1): 32-42.
[6]	ZOU Cheng-gong FENG Hao CHEN Bing TANG Hui SHAO Chuan SUN Mou YANG Rong HE Jia-quan . Research on the dynamic changes of neurological dysfunction and cognitive function impairment in traumatic brain injury [J]. Acta Anatomica Sinica, 2024, 55(1): 43-48.
[7]	LÜ Hai-yan SHEN Xi-ya ZHAO Fu-sheng ZHU Mei . Effects of tricholoma matsutake polysaccharides on 1-methy-4-pehnyl-pyridine ion-induced PC12 cell damage [J]. Acta Anatomica Sinica, 2024, 55(1): 49-54.
[8]	BAI He TENG Ye FENG Lei MENG Hai-wei1 TANG Yu-chun LIU Shu-wei. 3D hippocampal segmentation based on spatial and frequency domain features adaptive fusion and inter-class boundary region enhancement [J]. Acta Anatomica Sinica, 2024, 55(1): 73-81.
[9]	ZHENG Li-ping LIU Xia DU Xiao-hua WU Ya-juan LIU Shan-shan . Expression and distribution of brain-derived neurotrophic factor in different cerebrum regions of yak and cattle [J]. Acta Anatomica Sinica, 2024, 55(1): 10-16.
[10]	YIN Shi-qin YANG Si-yi WANG Rui-han YOU Gui-xuan YANG Ying-qiu ZHANG Lei. Distal tibiofibular syndesmosis fibular notch typing and its clinical significance based on CT [J]. Acta Anatomica Sinica, 2024, 55(1): 82-87.
[11]	YANG Yang SHI Jun LI Kun MA Yuan ZHANG Shao-jie HOU Er-fei WANG Chao-qun CHEN Jie WANG Xing LI Zhi-jun. Finite element analysis of cervical intervertebral discs after removing different ranges of uncinate processes [J]. Acta Anatomica Sinica, 2024, 55(1): 88-97.
[12]	SUN Lei WANG Xing-yu XIE Shui-hua. Risk analysis of re-fracture after percutaneous kyphoplasty in elderly patients with osteoporotic thoracolumbar compression fractures and construction of acolumnar graph prediction model#br# [J]. Acta Anatomica Sinica, 2024, 55(1): 98-104.
[13]	HAO Yong-ming GUO Lei ZHOU Cheng-hui PEI Han-jun LI Li ZHAO Zhe . Establishment of a rat model of myocardial hypertrophy by a modified abdominal aortic coarctation method [J]. Acta Anatomica Sinica, 2024, 55(1): 120-124.
[14]	XU Ya-ping YU Yue-xin CHEN Jin-fu WANG Ke-ke GUO Zhikun . Structural distribution and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats [J]. Acta Anatomica Sinica, 2023, 54(6): 716-721.
[15]	LIU Li-ping ZHU Meng-ni XU Hui . Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats [J]. Acta Anatomica Sinica, 2023, 54(6): 722-729.