Acta Anatomica Sinica

AAS ›› 2014, Vol. 45 ›› Issue (1): 47-52.doi: 10.3969/j.issn.0529-1356.2014.01.009

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Compatibility study of bone mesenchymal stem cells and ferulic acid treatment PHBV film in vitro

SONG Zhang-shuo1 BAI Shu-ling 1* PAN Feng2 TIAN Xiao-hong1 TONG Hao1   

  1. 1.Department of Tissue Engineering，College of Basic Medical Sciences，China Medical University，Shenyang 110001，China;2. Department of Human Anatomy, College of Basic Medical Sciences，China Medical University，Shenyang 110001，China
  • Received:2013-01-04 Revised:2013-09-11 Online:2014-02-06 Published:2014-02-06
  • Contact: BAI Shu-ling E-mail:baishuling@hotmail.com

Abstract:

Objective To evaluate the growing condition of bone mesenchymal stem cells (BMSCs) which were cultivated in the film of different concentration in the ferulic acid treatment of abamectin poly-3-hydroxy butyrate-co-3-hydroxy valerate(PHBV), and to investigate biocompatibility between the membrane of poly-3-hydroxy butyrate-co-3-hydroxy valerate and bone mesenchymal stem cells to provide a basis for the choice of scaffold material of bone tissue engineering. Methods The BMSCs were harvested by a whole bone marrow culture method,and identified by flow cytometric to analysis detection cell surface antigen. The BMSCs of the third passage were inoculated on the material surface and cultured for 8 hours,12 hours and 24 hours. Changes in form of BMSCs on different concentrations in the ferulic acid treatment surfaces were observed by scanning electron microscopy(SEM).The material leaching liquor to culture BMSCs was prepared and material was detected with the influence of cells by MTT and flow cytometer cell cycle assays method. Results The BMSCs were successfully cultured, these cells were uniformly negative for CD44，CD90 and positive for CD34，CD11b.SCM showed that PHBV material surface coarse, visible crystals.The cells on the group of a and b material surface growed well,and proliferated significantly.The MTT analysis and flow cytometer cell cycle assays showed that the PHBV film of 5% ferulic acid treatment promoted the growth and proliferation of BMSCsin vitro. Conclusion The PHBV film with 5% ferulic acid treatment had good biocompatibility of BMSCs. Environment is conducive to cell growth, adhesion and proliferation in vitro.

Key words: Bone mesenchymal stem cells, 3-(4-hydroxy-3-methoxyphenyl)-2-propenoic acid, Ferulic acid, Bone tissue engineering, Scanning electron microscopy, Rat