AAS ›› 2014, Vol. 45 ›› Issue (3): 328-332.doi: 10.3969/j.issn.0529-1356.2014.03.006

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Expressions of gene Fmr1 in rat cortex, hippocampus and thalamus areas after the rapid eyes movement sleep deprivation

FAN Gui-min1 WU Dong-mei2 WANG Pei-jun1 XIONG Hui1 YANG Ying-feng3 FAN Hong-ru4 WANG Jin-tao 1*   

  1. 1.Department of Anatomy, Basic Medical College, Jiamusi University,Heilongjiang Jiamusi 154007,China; 2.Basic Chemistry of College of Pharmacy, Jiamusi University, Heilongjiang Jiamusi 154007, China;3.Department of ICU,the First Affiliated Hospital of Jilin University,Changchun 130021,China;4.Department of Brotherhood Microsurgery, the First People’s Hospital of Jilin City,Jilin Jilin 132000,China
  • Received:2013-10-25 Revised:2014-02-19 Online:2014-06-06 Published:2014-06-06
  • Contact: WANG Jin-tao E-mail:1141107097@qq.com
  • Supported by:

    1. (LBH - Z09006) postdoctoral fund in heilongjiang province 2. (D2007-15) natural fund in heilongjiang province

Abstract:

Objective To investigate the expression of gene Fmr1 in rats cortex, hippocampus and hypothalamus areas after the rapid eyes movement (REM) sleep deprivation.
Methods Using the modified multiple platform method (MMPM), 126 rats were randomly and averagely divided into three groups, the normal control group (CC), the environmental control group (TC) and the sleep deprivation group (SD). Each group was detected on day 1, day 2, day 3, day 5, day 7, and day 9, and the sample tissues were extracted from 7 rats at each time point. Immunohistochemistry and RT-PCR were operated to analysis the expression of gene Fmr1.Results The expressions of gene Fmr1 were increased gradually in the cortex and thalamus of the SD group after 3 days (P<0.05), and the expressions in the CC and TC groups had no significant difference (P>0.05). The expressions of gene Fmr1 were decreased gradually in hippocampus for SD after 3 days (P<0.05), and that in the CC and TC groups had no significant difference (P> 0.05). Conclusion The expressions of gene Fmr1 were increased gradually in the cortex and thalamus but decreased in the hippocampus in the SD group after 3 days.

Key words: Rapid eyes deprivation, Gene fmr1, Immunohistochemistry, Rat