Angiotensin Ⅱ promotes fibroblasts phenotypic transformation through down-regulating adventitial catalase expression

doi:10.3969/j.issn.0529-1356.2014.03.021

Abstract

Abstract:

Objective To investigate the relationship between angiotensin Ⅱ (AngⅡ) and transformation of vascular fibroblasts phenotype.Methods Eighteen rats were randomly assigned into the untreated group, mini-pump infusion of saline group and mini-pump infusion of AngⅡ group which was used as the hypertension model. Their systolic pressure and vascular morphology were examined. The expression of catalase (CAT) and 4HNE was examined by immunohistochemistry. Western blotting was used to examine the expression of CAT of adventitial fibroblasts which were cultured by different incubation times and concentrations of AngⅡ. Results Compared with untreated and mini-pump infusion of saline groups, the systolic pressure and carotid media thickness stained by HE of mini-pump infusion of AngⅡ group were significantly higher(P<0.01).The results of immunohistochemistry showed that the expression of CAT ofAngⅡ group was significantly lower than that of untreated group , however the expression of 4HNE of AngⅡ groupwas higher than that of untreated group (P<0.05). Furthermore, the results of Western blotting indicated that the effect of Ang Ⅱ on down-regulation of CAT function was in a dose and incubation time dependent manner. Conclusion AngⅡ down-regulates adventitial CAT expression and promotes fibroblasts phenotypic transformation which leads to pathological arterial vascular remodeling.

Key words: Angiotensin Ⅱ, Catalase, Phenotype, Transformation, Fibroblast, Western blotting, Rat

SHEN Kai LIN Zhuo-ming CHEN Shi-liang YUAN Guo-yu LIU Xiao-guang*. Angiotensin Ⅱ promotes fibroblasts phenotypic transformation through down-regulating adventitial catalase expression[J]. AAS, 2014, 45(3): 411-415.

References

［1］Havelka GE, Kibbe MR. The vascular adventitia: its role in the arterial injury response［J］.Vasc Endovascular Surg,2011, 45(5): 381-390.
［2］Panza JA, Casino PR,Kilcoyne CM,et al. Role of endothelium-derived nitric oxide in the abnormal endothelium-dependent vascular relaxation of patients with essential hypertension［J］. Circulation,1993,87（50）:1468-1474.
［3］Liu J, Ormsby A, Oja-Tebbe N，et al. Gene transfer of NAD(P)H oxidase inhibitor to the vascular adventitia attenuates medial smooth muscle hypertrophy［J］. Circ Res, 2004,95(6): 587-594.
［4］Herrmann J, Samee S, Chade A, et al. Differential effect of experimental hypertension and hypercholesterolemia on adventitial remodeling［J］. Arterioscler Thromb Vasc Biol, 2005, 25(2): 447-453.
［5］Birukov KG. Cyclic stretch, reactive oxygen species, and vascular remodeling［J］. Antioxid Redox Signa, 2009,11(7):1651-1667.
［6］Maiellaro K, Taylor WR. The role of the adventitia in vascular inflammation［J］. Cardiovasc Res, 2007,75（4）:640-648.
［7］Barnes L, Gorin Y. Myofibroblast differentiation during fibrosis: role of NAD(P)H oxidases［J］. Kidney Int, 2011, 79(9): 944-956.
［8］Csanyi G, Taylor WR, Pagano PJ. NOX and inammation in the vascular adventitia［J］. Free Radic Biol Med, 2009, 47(9): 1254-1266.
［9］Wang HX, Yang H, Han QY, et al. NADPH oxidases mediate a cellular “memory” of angiotensin Ⅱ stress in hypertensive cardiac hypertrophy［J］. Free Radic Biol Med, 2013,65:897-907.［10］Savoia C, Burger D, Nishigaki N, et al. Angiotensin Ⅱ and the vascular phenotype in hypertension［J］. Expert Rev Mol Med, 2011,13:e11.
［11］Weaver M, Liu J, Pimentel D, et al. Adventitial delivery of dominant-negative p67phox attenuates neointimal hyperplasia of the rat carotid artery［J］. Am J Physiol Heart Circ Physiol, 2006,290(5):H1933-1941.
［12］Cascino T, Csanyi G, Al Ghouleh I, et al. Adventitia-derived hydrogen peroxide impairs relaxation of the rat carotid artery via smooth muscle cell p38 mitogen-activated protein kinase［J］. Antioxid Redox Signal,2011, 15(6): 1507-1515.
［13］Sumners C, Fleegal MA, Zhu M. Angiotensin ATl receptor signailing pathways in neurons［J］. Clin Exp Pharmacol Physiol, 2002, 29(5-6): 483-490.
［14］Lob HE, Schultz D, Marvar PJ, et al. Role of the NADPH oxidases in the subfornical organ in angiotensin Ⅱ-induced hypertension［J］. Hypertension, 2013,61(2):382-387.

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