Expression of the receptor for advanced glycation end products in mice with chronic adriamycin-induced cardiotoxicity

doi:10.3969/j.issn.0529-1356.2014.04.020

Abstract

Abstract:

Objective To study the expression of receptor for advanced glycation end products (RAGE) in chronic adriamycin (ADR)-induced cardiotoxicity mice. Method A mouse model of chronic cardiotoxicity was established by intraperitoneal injection of 5 mg/kg ADR once a week for continuous 3 weeks. Mice treated with the same volume of saline were used as control group (6 mice in each group). Echocardiography and histopathological examination were used to evaluate the myocardial injury, cardiac function and ventricular remodeling. The expression of RAGE in cardiac tissues was detected by immunohistochemical staining and Western blotting. Results Echocardiography indicated that ADR-treated mice had reduced left ventricular ejection fraction, while histopathological analysis of cardiac tissues showed obvious cardiac damage. RAGE protein in cardiac tissues was highly expressed in chronic ADR-induced cardiotoxicity mice. Conclusion RAGE is highly induced and may be involved in the cardiotoxicity after ADR treatment.

Key words: Adriamycin, Receptor for advanced glycation end products, Myocardium, Echocardiography, HE staining, Immunohistochemistry, Western blotting, Mouse

SHI Sheng-feng ZHU Jin-zhou ZHANG Rui-yan*. Expression of the receptor for advanced glycation end products in mice with chronic adriamycin-induced cardiotoxicity[J]. AAS, 2014, 45(4): 550-554.

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